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Showing: 1 - 20 of 23 results

  1. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Neurons in the Periaqueductal Gray Facilitate the Itch-Scratching Cycle via Article Tac1-Expressing Neurons...Neurons in the Periaqueductal Gray Facilitate the Itch-Scratching Cycle via Descending Regulation. Neuron...nucleus accumbens medial shell and behavioral switching. Neurochemistry International 129:104482. PubMed...

  2. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Plasmids: pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA, & FBL-...97.2 KB Yamamoto PITCh Tagging System The Takashi Yamamoto lab has deposited the PITCh system plasmids ...vectors as well as pX330S-2-PITCh, expressing a generic gRNA targeting the PITCh donor plasmid. Note that...FBL-specific gRNA pCRIS-PITChv2-FBL - PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus...cassette to the C-terminus of endogenous proteins. The PITCh system depends on MMEJ (microhomology-mediated end-joining...targeting the genomic site and the donor vector. The PITCh donor plasmid with an EGFP-2A-PuroR cassette, flanked...sequences and gRNA target sites. The first deposited PITCh plasmids were tested by fusing EGFP-2A-PuroR cassette...

  3. Cre-lox system

    Type
    Collection
    ...interest makes FLEx switch a powerful experimental tool. For more information on FLEx switch and what you can...Hu 154018 LiOn-CMV∞Cre leak-proof iOn (LiOn) Cre switch CMV Mammalian Livet loxP (floxed) Constructs These... Cre expression promotes shRNA expression. Gene Switch: These constructs contain two genes of interest...frame to allow in-frame translation of Gene B. FLEx Switch ∗ : This system allows scientists to utilize recombination...while simultaneously turning on another. The FLEx switch system takes advantage of the orientation specificity...Mouse Targeting Shivdasani 18925 pJ241-FLEX FLEX switch empty backbone Sternson 38042 pAAV-CA-FLEX Cre ...Mammalian Cepko 8389 p212 pCMV-EGFP/RFP EGFP-dsRed gene switch plasmid Mammalian Green 22799 Ai9 Rosa26 targeting...

  4. Optogenetics Plasmid Collection

    Type
    Collection
    ...optogenetics plasmids such as microbial opsin and optical switch tools to measure and manipulate cells and their... Protein Guide Microbial Opsin Plasmids Optical Switch Plasmids Background Optogenetics plasmids encode...proteins. These fusions can then be used as an optical switch to control protein interactions, dimerization, ...spectra) of different types of opsins and optical switches available in these tables, refer to Addgene's ...Type Promoter PI Publication Return to top Optical Switch Plasmids Browse, sort, or search the table below...

  5. Brain Initiative Collection

    Type
    Collection
    ... Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow...parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades...parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades...

  6. Biosensor AAV Preps

    Type
    Collection
    ... Tian Calcium Sensor: Twitch2B 100040 pAAV.hSyn1.Twitch2B.WPRE.SV40 Syn Twitch2B none Constitutive 1 Griesbeck...ribo-GCaMP8 GCaMP7 GCaMP6 axon-GCaMP6 soma-GCaMP6 Twitch2B Yellow Calcium Sensors jYCaMP1 axon-jYCaMP1 Red...

  7. Retrograde AAV viral preps

    Type
    Collection
    ...category by using the buttons. Controls Green Red Switch Other Recombinases Cre Flp VCre Dre Calcium Sensors...EF1a Nuc-flox(mCherry)-EGFP EF1a Color-flipping switch. Expresses NLS-mCherry in the absence of Cre, and...

  8. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...researchers have begun to use anti-CRISPRs as off-switches for CRISPR genomic editing. Genome Engineering...KL, Davidson AR. 2016. Naturally occurring off-switches for CRISPR-Cas9. Cell . 167(7):1829-1838. PMID...

  9. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Stokes Shift Photoactivatable Photoconvertible Photoswitchable Timers Blue/UV Protein Excitation (nm) Emission...pBAD-PSmOrange2 - Bacterial Expression Jump to Top Photoswitchable (e.g. off to on to off) Protein Excitation ...

  12. Synthetic Biology - Overview

    Type
    Collection
    ...Sigma Factors , Resource Allocator and Orthogonal Switches Other Addgene Tools and Resources CRISPR Fluorescent...

  13. Antibody Plasmid Collection

    Type
    Collection
    ... secrete antibodies as Fab' fragments, isotype-switched chimeric antibodies, and Fc-silent mutants. Functional...

  14. mTOR Pathway

    Type
    Collection
    ...nutritional state of the cell and regulates the switch from anabolic to catabolic processes. Active mTORC1...

  15. Control AAV Preps

    Type
    Collection
    ...Fluorophore/Tag Green Red None Other Cre-dependent color switch Brainbow constructs Spaghetti monster tags Activity...

  16. Ras Pathway

    Type
    Collection
    ...binding protein) family. GTPases serve as molecular switches that transmit signals within the cell. GTPases...

  18. Rett Syndrome

    Type
    Collection
    ...reversible in mice if MECP2 gene expression is switched on regardless of age or symptom severity, suggesting...

  19. Bacterial Expression Systems

    Type
    Collection
    ... Photoactivatable , Photoconvertible , and Photoswitchable fluorescent proteins Various PIs Fluorescent...
Showing: 1 - 20 of 23 results