...Kv) channels fused to LOV2 photoswitch. Illumination causes the photoswitch to unfold, lowering the toxin's...including explanations of microbial opsin and optical switch terminology. Science...Optogenetics Guide Introduction Microbial Opsins Optical Switches References Introduction The field of optogenetics...of proteins; e.g., microbial opsins and optical switches Sensors are genetically-encoded reporters of molecular...inhibitory neurons near the injection site. Optical Switches Researchers have harnessed plant and bacterial...protein systems controlled by light. These “photoswitchable” proteins offer exquisite spatial and temporal...through directed mutagenesis - use Addgene's Optical Switch Plasmid Table to search for plasmids for your next...

...Gibson Type IIS Ligation Independent Cloning Oligo Stitching Molecular cloning or the creation of recombinant...expensive. In addition it is quite difficult to switch to another cloning method, such as restriction ...Top Yeast-mediated Cloning and Oligonucleotide Stitching Yeast-mediated cloning is very similar in principle...advantage is the ability to perform oligonucleotide stitching, in which pieces of DNA that share no end homology...

...receptor in unwanted cell types. Generating a FLEx switch to control expression of a chemogenetic reporter...

...Davidson, A. R. (2016). Naturally occurring Off-Switches for CRISPR-Cas9. Cell , 167 (7), 1829-1838.e9....