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...Kv) channels fused to LOV2 photoswitch. Illumination causes the photoswitch to unfold, lowering the toxin's...including explanations of microbial opsin and optical switch terminology. Science...To: Optogenetics Guide Microbial Opsins Optical Switches Plan Your Experiment References The field of optogenetics... cells, including microbial opsins and optical switches. For more information on biosensors such as calcium...preparations of many optogenetics plasmids. Optical Switches Researchers have harnessed plant and bacterial...protein systems controlled by light. These "photoswitchable" proteins offer exquisite spatial and temporal...tools through directed mutagenesis. Find optical switches in Addgene's Optogenetics Plasmid Collection for...

...Ligation Independent Cloning Yeast-Mediated & Oligo Stitching Resources Molecular cloning, or the creation of...expensive. In addition, it is quite difficult to switch to another cloning method, such as restriction ...BioRender.com. Yeast-mediated Cloning and Oligonucleotide Stitching Yeast-mediated cloning is very similar in principle...advantage is the ability to perform oligonucleotide stitching, in which pieces of DNA that share no homology...-mediated plasmid cloning and oligonucleotide stitching. Created with BioRender.com. Additional Resources...

...assembling large DNA fragments based on methylation-switching. 4G-Cloning Plasmid Kit Bacterial Expression, ...

...receptor in unwanted cell types. Generating a FLEx switch to control expression of a chemogenetic reporter...

...Davidson, A. R. (2016). Naturally occurring Off-Switches for CRISPR-Cas9. Cell , 167 (7), 1829-1838.e9....