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  1. pAAV.hSyn1.Twitch2B.WPRE.SV40 (AAV1)

    Viral Prep
    #100040-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.hSyn1.Twitch2B.WPRE.SV40 (#100040). In addition to the viral particles, you will also receive purified pAAV.hSyn1.Twitch2B.WPRE.SV40 plasmid DNA. Synapsin-driven expression of (FRET)-based calcium biosensor Twitch2B. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Oliver Griesbeck
    Article
    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm
    Promoter
    Syn
    Tags
    None
    Available since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only

  2. ITCH

    Plasmid
    #36197
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ITCH (ITCH Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains HECT domain
    Promoter
    Available since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. 5C7M (ITCH)

    Plasmid
    #69966
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ITCH:SDC094-D06:C29524 (ITCH Human)
    Use
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. SpySwitch

    Plasmid
    #184225
    Purpose
    Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.
    Depositor
    Mark Howarth
    Article
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    SpySwitch
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    E77A mutation prevents isopeptide bond formation,…
    Promoter
    T7
    Available since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184225-plasmid-map-sequence-id-361604

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  11. UAS:bloSwitch

    Plasmid
    #108355
    Purpose
    UAS driven green to red switch reporter for B3 recombinase expression
    Depositor
    Harold Burgess
    Article
    Tabor et al Curr Biol. 2018 Aug 20;28(16):2527-2535.
    Insert
    UAS:[Ubiquitin-C-intron]-[blown-out site]-nuclear-GFP-[beta-globin-polyA]-[blown out site]-[membrane TagRFPT]
    Use
    Zebrafish expression
    Tags
    Expression
    Mutation
    Zebrafish codon optimized
    Promoter
    lac
    Available since
    Sept. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    108355-plasmid-map-sequence-id-208272

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  19. pP{ELAV-GeneSwitch}

    Plasmid
    #83957
    Purpose
    Expresses conditional RU486-dependent GAL4 protein under control of the elav promotor
    Depositor
    Haig Keshishian
    Article
    Osterwalder et al Proc Natl Acad Sci U S A. 2001 Oct 23;98
    Insert
    fusion protein consisting of the GAL4-DBD, human progesterone receptor-ligand-binding domain, and P65-AD (GAL4, RELA Budding Yeast, Human)
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    elav
    Available since
    Dec. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    83957-plasmid-map-sequence-id-160516

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Showing: 1 - 20 of 6039 results