...and CIB1 – which they borrowed from Arabidopsis thaliana. The results are light-inducible transcriptional...those optogenetic controls, gene expression levels reliably increased in vitro by a factor of 10 to 20. A ... Silvana Konermann et al. "Optical control of mammalian endogenous transcription and epigenetic states...

...Nihongaki et al., 2015   CRY2 and CIB1 from A. thaliana dCas9 Activation of gene transcription Polstein...Polstein et al., 2015 LACE CRY2 and CIB1 from A. thaliana dCas9 Activation of gene transcription Nihongaki... al., 2017 Split-CPTS2.0 CRY2 and CIB1 from A. thaliana dCas9 Activation of gene transcription Kim et... et al., 2019 LADL CRY2 and CIB1 from A. thaliana dCas9 Activation of gene transcription. Label genomic...

...two of these recombinases were also active in mammalian cells. In experiments, B3 and KD were shown to... and DNA damage induced by Cre recombinase in mammalian cells. Loonstra A, Voojis M, Beverloo HB, Al Allak.../s004270100167. PubMed. Cryptic loxP sites in mammalian genomes: genome-wide distribution and relevance...

...within the expression system (bacteria, yeast, mammalian, etc.). To individually isolate a protein, there...more straightforward and of a higher purity than mammalian purifications due to the presence of more naturally...occurring histidine-rich proteins in mammals. Mammalian purification is still possible but requires optimization...

...common cell type within the kidney—either endothelial, epithelial, or fibroblast. However, Frank Graham showed...blog Five Popular Model Organisms 10 Tips for Mammalian Cell Culture Adenoviral Vector Production and...

... factor IX in liver in a mouse model of Haemophilia B (Meliani et al., 2017). Exo-AAV9 was successfully...PMID: 22314290. PubMed Central PMCID: PMC3345986. Meliani, Amine, et al. "Enhanced liver gene transfer and...

...little more this October. Science favorites Musicophilia: Tales of Music and the Brain by Oliver Sacks...properties it has. As with other works of Dr. Sacks, Musicophilia includes case studies of relevant neurological...book! Far from a work of glib pop psychology, Musicophilia is accessible and fascinating, all without compromising...

...feel it. But the same features that make these familiar viruses such a pain is exactly why viruses are... There are four main types of viruses used in mammalian research: 𝛄-retrovirus, lentivirus, adenovirus...approved in the US for multiple diseases, including hemophilia B and spinal muscular atrophy.   Type of Virus...

...expression vectors are available at Addgene. A set of mammalian expression vectors containing various spike protein...modification may not be easily expressed in non-mammalian cells. Yeast expression plasmids The yeast expression...cleavage, codon usage, and expression system. Mammalian expression plasmids Finally a set of wild type...

...Ladder Engineered miniature CRISPR-Cas system for mammalian genome regulation and editing Anti-FLAG Frankenbodies...x Engineered miniature CRISPR-Cas system for mammalian genome regulation and editing by: Rachel Leeson...and endogenous genes. It is highly specific in mammalian cells without significant off-target effects, ...

...specific editor in mammalian cells Not all Cas13d proteins are functional in mammalian cells, but Konermann... does not occur when Cas13d is expressed in a mammalian system. Since Cas13d is functionally similar to...

...significantly improve the reproducibility and reliability of science publications. Peer reviewing To make...make sure your manuscript is perceived as a reliable source of information, it is best to submit it to... is completely open; the reviewer’s name and affiliations, and the entire referee report are permanently...

...to his CRISPR/Cas9 system. Addgene: For people unfamiliar with this new TALEN kit, can you describe it ...one can introduce changes into the genomes of mammalian or other cells. TALENs are engineered proteins...will bind desired sequences. TALENs are much more reliable. If you design them for a particular sequence,...

... and have a diverse range of functions within mammalian cells. Here, we will cover how these structures...putting DNA at risk. Consequences of duplexed RNA Mammalian cells have multiple receptors in place to sense...strands to re-pair. The two dominating RNases in mammalian cells (RNase H1 and H2) handle most of these intermediates...

... collection to perform a functional screen in mammalian cells to identify any solute channels that can...team will be able to validate their findings in mammalian cells with both human and murine targets thus ...says. In induced pluripotent stem cells derived endothelial cells from women who are missing an entire X ...

...research technician in Kaspar Podgorski’s lab at Janelia Research Campus, HHMI where he works on developing...collaborative and highly interactive environment that Janelia offers,” says Aggarwal.In addition to the biosensors...pooled library of mutant sensors expressed in a mammalian cell line engineered to be electrically excitable...

...supplements), suggested sequencing primers, or mammalian selection markers. You don’t need a fancy database...feature you want to verify because you won’t get reliable sequence data back for the first 50-100 base pairs... Julian Taylor-Parker...

...neuron-specific expression (hSyn promoter) and pan-mammalian cell expression (CAG promoter). An improved version...neuron-specific expression in both zebrafish and mammalian systems. The GCaMP6, jGCaMP7, and jGCaMP8 sensors...neuron-specific, astrocyte-specific (GCaMP6 only) or mammalian cell expression, and compatible with constitutive...

...system can be used in many cell types including endothelial cell-pericyte, neuron-astrocyte, and cancer-stromal...induce DSBs within minutes. New CRISPR plasmids for Borrelia burgdorferi from Christine Jacobs-Wagner’s lab...enzymes. CRISPR-ARE is a new system from Jiazhang Lian’s lab that enables simultaneous gene activation, ...

...novel techniques or to engineer improved tools for familiar applications. But how does one invent a tool for...Fusing EGFP to the self-labeling FlavinTag made a reliable one-component magnetoresponsive system that could...clearly visible whether expressed in E. coli, in mammalian cells, or in purified form, and didn’t need any...