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We narrowed to 532 results for: lias

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Showing: 101 - 120 of 532 results

  1. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Backbones Mammalian CMV, SV40, EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian expression...or miniTurbo in the mammalian nucleus pSF3-ultraID - Expresses ultraID in mammalian cells with the Tet-...Independent Cloning (LIC) pAG CMV Empty Puro - Empty mammalian expression plasmid for cloning untagged proteins...proteins pcDNA3.1-mCherry - Expresses mCherry tag in mammalian cells pBI-MCS-EGFP - Tet-inducible Find more Tet-inducible...FLAG-HA-pcDNA3.1 - C-terminal Flag or N-terminal FLAG-HA for mammalian expression pNIC-CTHF - C-terminal ...including Xenopus HA Epitope tag pcDNA3.1-HA - Mammalian expression vector with N-terminal HA tag pInducer20...Myc Epitope tag pKMyc - N-terminal Myc tag for mammalian expression pGEX-4T-1-3xMyc - Bacterial vector ...

  2. Typing CRISPR Systems

    Type
    Blog Post
    ...effector and is able to target and edit RNA in mammalian cells. Type IV Type IV is the middle child (like...effector and are likely the class most people are familiar with. Class 2 CRISPR systems can be further broken.... Cas9 was the first to be adapted for use in mammalian systems, with the landmark publication in 2013...Loci in Integrative Conjugative Elements of Acidithiobacillia. The CRISPR Journal, 4(5), 656–672. https:...

  3. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...Differential cytosine editing between bacteria and mammalian cells Researchers in the Xueli Zhang lab were ...called a Glycosylase Base Editor, or GBE, for its reliance on the glycosylase UNG to crease abasic sites ...seemed to follow the same initial mechanism as in mammalian cells — by excising the freshly edited U bases...Huang, M. E., Qin, Y., Shang, Y., Hao, Q., Zhan, C., Lian, C., Luo, S., Liu, L. D., Zhang, S., Zhang, Y., ...

  4. Caltech Systemic Capsids

    Type
    Collection
    ...AiP13044 - pAAV-AiE0779m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3044) minBG SYFP2 Control AIBS , Ting 191707 ...AiP12237 - pAAV-AiE0452h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2237) minBG SYFP2 Control AIBS , Ting 191708 ...AiP12700 - pAAV-AiE0452h-minBG-mTFP1-WPRE3-BGHpA (Alias: CN2700) minBG mTFP1 Control AIBS , Ting 191720 ...AiP13038 - pAAV-AiE0743m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3038) minBG SYFP2 Control AIBS , Ting 191726 ...AiP12787 - pAAV-AiE0140h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2787) minBG SYFP2 Control AIBS , Ting 191728 ...AiP12408 - pAAV-AiE0600m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2408) minBG SYFP2 Control AIBS , Ting 191737 ...AiP12610 - pAAV-AiE0780m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2610) minBG SYFP2 Control AIBS , Ting 199773 ...

  5. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    ...deliver both Cas9 and your gRNA of interest to mammalian cells. It was designed to have increased lentiviral...libraries targeting both mouse and human cells are avaliable for distribution. To learn more about pooled library...Some Common Ways to Deliver CRISPR Plasmids to Mammalian Cells Resources on the Addgene Website Visit...

  6. Let There Be LITE Plasmids

    Type
    Blog Post
    ...and CIB1 – which they borrowed from Arabidopsis thaliana. The results are light-inducible transcriptional...those optogenetic controls, gene expression levels reliably increased in vitro by a factor of 10 to 20. A ... Silvana Konermann et al. "Optical control of mammalian endogenous transcription and epigenetic states...

  7. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...Nihongaki et al., 2015   CRY2 and CIB1 from A. thaliana dCas9 Activation of gene transcription Polstein...Polstein et al., 2015 LACE CRY2 and CIB1 from A. thaliana dCas9 Activation of gene transcription Nihongaki... al., 2017 Split-CPTS2.0 CRY2 and CIB1 from A. thaliana dCas9 Activation of gene transcription Kim et... et al., 2019 LADL CRY2 and CIB1 from A. thaliana dCas9 Activation of gene transcription. Label genomic...

  8. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    ...two of these recombinases were also active in mammalian cells. In experiments, B3 and KD were shown to... and DNA damage induced by Cre recombinase in mammalian cells. Loonstra A, Voojis M, Beverloo HB, Al Allak.../s004270100167. PubMed. Cryptic loxP sites in mammalian genomes: genome-wide distribution and relevance...

  9. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...within the expression system (bacteria, yeast, mammalian, etc.). To individually isolate a protein, there...more straightforward and of a higher purity than mammalian purifications due to the presence of more naturally...occurring histidine-rich proteins in mammals. Mammalian purification is still possible but requires optimization...

  10. Luciferase Plasmid Collection

    Type
    Collection
    ...Luciferase-pcDNA3 Firefly CMV Mammalian expression of firefly luciferase William Kaelin 60495 pSBtet-GP Firefly... CMV Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla CMV Mammalian expression...Gaussia luciferase (Gluc) NanoLuc® (Nluc) The mammalian codon-optimized version of firefly luciferase ...noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s) Description...of a CMV promoter is present for normalization William Kaelin 71248 pXPG Firefly Insertion of 5' promoter... expression of luciferase in the cytoplasm of mammalian cells Peter Cockerill 212936 pGL3 Basic Vector...Firefly Creation of N-terminal Firefly fusions in Drosopholia Michael Rosbash 128046 pGL3basic luciferase and...

  11. What the HEK?

    Type
    Blog Post
    ...common cell type within the kidney—either endothelial, epithelial, or fibroblast. However, Frank Graham showed...blog Five Popular Model Organisms 10 Tips for Mammalian Cell Culture Adenoviral Vector Production and...

  12. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...little more this October. Science favorites Musicophilia: Tales of Music and the Brain by Oliver Sacks...properties it has. As with other works of Dr. Sacks, Musicophilia includes case studies of relevant neurological...book! Far from a work of glib pop psychology, Musicophilia is accessible and fascinating, all without compromising...

  13. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ... factor IX in liver in a mouse model of Haemophilia B (Meliani et al., 2017). Exo-AAV9 was successfully...PMID: 22314290. PubMed Central PMCID: PMC3345986. Meliani, Amine, et al. "Enhanced liver gene transfer and...

  16. Hot Plasmids - February 2022

    Type
    Blog Post
    ...Ladder Engineered miniature CRISPR-Cas system for mammalian genome regulation and editing Anti-FLAG Frankenbodies...x Engineered miniature CRISPR-Cas system for mammalian genome regulation and editing by: Rachel Leeson...and endogenous genes. It is highly specific in mammalian cells without significant off-target effects, ...

  17. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...feel it. But the same features that make these familiar viruses such a pain is exactly why viruses are... There are four main types of viruses used in mammalian research: 𝛄-retrovirus, lentivirus, adenovirus...approved in the US for multiple diseases, including hemophilia B and spinal muscular atrophy.   Type of Virus...

  18. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...significantly improve the reproducibility and reliability of science publications. Peer reviewing To make...make sure your manuscript is perceived as a reliable source of information, it is best to submit it to... is completely open; the reviewer’s name and affiliations, and the entire referee report are permanently...

  19. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...to his CRISPR/Cas9 system. Addgene: For people unfamiliar with this new TALEN kit, can you describe it ...one can introduce changes into the genomes of mammalian or other cells. TALENs are engineered proteins...will bind desired sequences. TALENs are much more reliable. If you design them for a particular sequence,...

  20. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...GG-acceptor Mammalian hU6 epegRNA BsaI No mRFP1 David Liu 174039 pU6-tmpknot-GG-acceptor Mammalian hU6 epegRNA...CRISPR Prime Edit CRISPR Plasmids: Prime Edit Mammalian Bacteria Drosophila Plant Empty pegRNA Resources... other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker...Cas9 Selection PI 132777 pU6-pegRNA-GG-acceptor Mammalian hU6 pegRNA BsaI No mRFP1 David Liu 140448 QPM-... No Vermilion Norbert Perrimon 164423 pHSG1C3 Mammalian U6 pegRNA + nicking sgRNA BbsI for sgRNAs; BbsI...sgRNA No Tilmann Weber 173220 pPBT-peRNA_GG-Puro Mammalian, piggyBac hU6 pegRNA BsmBI No Puromycin Jacob ...Giehm Mikkelsen 173222 pPBT-PE2-PuroTK-pegRNA_GG Mammalian, piggyBac hU6 pegRNA BsmBI Yes (Cas9 H840A + MMLV...
Showing: 101 - 120 of 532 results