We narrowed to 878 results for: lic

...headings. Click on the publication link to view all plasmids available from the article. Click on the PI...Description Gene/Insert Antibiotic Resistance PI Publication Back to Top Do you have suggestions for other...

...headings. Click on the publication link to view all plasmids available from the article. Click on the PI...Plasmid Gene/Insert Vector Type Promoter Tags PI Publication Do you have suggestions for other plasmids that...

...and autism, and other hypotheses mount yet policies and public opinion do not seem to take note. For example...to inform the public (8–13). Facing the possibility that reason is losing ground in public discourse, many...Democrats more often than Republicans, the March is not about Democrats or Republicans (16). This is a Pro-...inclusion of science to inform policy (17). The facts surrounding a matter or policy should have more weight ... Idea Pew Research Center: Public Praises Science; Scientists Fault Public, Media March for Science: Principles...when we drop them. Scientific realities limit what policy can do and, in this way, science has always been...what outcomes are actually possible for a given policy. In exchange, the government protects (and often...

...headings. Click on the publication link to view all plasmids available from the article. Click on the PI... Plasmid Gene/Insert Vector Type Promoter PI Publication Back to Top Do you have suggestions for other...

...Technical Tips). Minigenome replication and transcription In order to replicate the minigenome, the viral...transcription and replication strategies of Marburg virus and Ebola virus by using artificial replication systems...allowing researchers to study aspects of the EBOV replication cycle under BSL-2 conditions (4). What is a ...that are crucial cis-acting signals for genome replication and viral gene transcription (6). The EBOV genome...5’ ends of the genome which are required for replication and transcription (Figure 1). All viral genes...cis-acting signals necessary to direct minigenome replication and reporter gene transcription by the EBOV polymerase... in 2, 5). The minigenome is capable of being replicated and transcribed in the cell when the appropriate...

... Promoter PI Publication Return to top Cut ID Plasmid Gene/Insert Promoter PI Publication Return to top...Promoter PI Publication Return to top Prime Edit ID Plasmid Gene/Insert Promoter PI Publication Return to...

...Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter PI Publication Yeast ID Plasmid...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids that should be added...

...expressed from plasmids. These antibodies undergo application-specific validation and consistent quality control...and provide experimental data for recommended applications. Addgene values transparency and accessibility...antibodies developed and validated for many research applications. Browse Antibodies Production Interested in ... storage techniques, and explain common applications. View Guide Need Some Help? Browse some of our...

...Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter...Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection...

...good quality application. Even following this advice, only about 50% of my grant applications have been ...do need to be able to research and write your application, then give it to someone else to read and / or...it). It’s easy for reviewers to spot when an application is rushed (whether the idea isn’t properly researched...researched / developed or the application is full of typos) and these are unlikely to get past the initial...someone else in the department) to read over your application and give you feedback. They’ve most likely been...alterations before submitting - handing them your application 3 days before the deadline isn’t going to be ...problem in a novel way. Finally, engineering new applications to solve problems will provide originality to...

...science is all about navigating conflict. In literary terms, conflict can be defined as (1) the struggle...story, you need to have conflict. There are a lot of ways to introduce conflict into your work, but one...problem?” Those are the conflicts that will drive your story forward.  Beyond the Conflict  Once you have your...that the audience can understand the conflict. A science-based conflict gives you an easy framework with ...presence of obstacles that need to be overcome. Conflict, and its inherent tension, is interesting. It’...invested in seeing how they do it.    Finding Conflict in Science  In all science communication, what...talk to a scientist about their research, these conflicts come out quite naturally as they explain their...

...signaling network for transitioning between anabolic and catabolic states. Ras Ras is a GTPase that is essential...classification and treatment. On each pathway page, click on a component to find plasmids with your gene of... described in the initial Target Accelerator publications, focused on somatic variants found in lung adenocarcinoma...

...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...together because they are intended for use as a set. Click here to view all kits. Kit Types Fluorescent Proteins... backbone and only differing in a small region. Click here to view all pooled libraries. Pooled Library...plasmids from a depositor organized by a common use. Click here to view all collections. Depositor Collection...

...Promoter PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to ...non-targeted strand, Cas3 starts unraveling DNA using its helicase properties, cleaving the targeted strand non-specifically...top Plant ID Plasmid Gene/Insert Promoter PI Publication Return to top CRISPR Resources Addgene has a ...

...Background noise Probe Allelic specificity Can be multiplexed Single amplicon validation technique ... and dsDNA. This is not true for all amplicons; if your amplicon is AT-rich or has a secondary structure...understanding and technical proficiency with common applications - now let’s dive right in! You know qPCR is...as it solves, so understanding and using the application correctly is imperative. Picking your chemistry...hairpin design of probes/primers often offer higher allelic specificity, due to the need for the target:probe...Bustin, 2017 for a more in-depth review.)  Your amplicon should be between 70-200 bp long With ΔΔCT analysis... intensity should smoothly increase as the DNA amplicon transitions from single-stranded to double-stranded...

...offer export license applications for VSV and SARS-COV-1 plasmids. Export control license process    An...excited to announce we now offer an export license application for rabies materials! Some of the materials...cannot expedite the application process.  More information about export control licenses is available through...the USA, where Addgene is located.  The export license, if granted, allows us to ship rabies plasmids ...  An export control license, once approved, allows Addgene to ship plasmids or viral vectors containing...will need to provide a brief description of the applicable research project when purchasing export-controlled...export-controlled materials. Addgene will then apply for the license for distribution to the requester’s organization...

... nuclease along with the guide RNA. For most applications, we recommend using Cas9 with the single guide...codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons (Ran et al., Nature Protocols 2013). Individual...SaCas9; SaCas9 sgRNA can be co-transfected as PCR amplicons using a protocol similar to SpCas9 sgRNA PCR (...function of genetic elements. To broadly enable the application of Cas9 in vivo and ex vivo , the Zhang Lab established...can be combined with Cas9 in a wide range of applications. Full references are below. The Cas9 knockin...

...  Limitations of the in silico approach The in silico approach cannot replicate the complexity of antigen... risk of an antibody.  In silico prediction of adverse reactions In silico assessment uses computer modeling...from the in silico assessments, as well as analyze the contributing factors that in silico prediction ... drugs are still immunogenic, meaning that they elicit an immune response from the body. Several factors...responses vary depending on the person. However, in silico and in vitro assessment of immunogenicity can help...drug epitopes likely to be presented by T cells to elicit an immune response. T cell epitopes are found in... evaluate the immunogenicity of therapeutics in silico. These algorithms are trained on vast amounts of...

...received 116,224 employment based green card applications and approved 91,023 during the first 3 quarters... development, business development, consulting, policy, big data and the list goes on and on. As you think...options discussed in this post benefit from the applicant thinking about the process early, keeping good...occupation" as requiring theoretical and practical application of a body of highly specialized knowledge in ...bachelor's degree or its equivalent and state licensure, if required to practice in that field. H-1B work-authorization...Hire American" Executive Order which sets broad policy intentions directing federal agencies to propose... April 1 deadline for companies to submit an application on their behalf.  If an individual obtains a ...

...that in a bit! Viral genomes and replication When it comes to replication, viruses tend to march to the ...methods of replication. In fact, an entire classification scheme, known as the Baltimore replication classes...developed around how they replicate (Figure 2). Figure 2: Baltimore replication classes. Image credit: ...an intermediary DNA replication step between DNA and mRNA. DNA genome replication can involve an RNA or...cell. The cell then uses its own machinery to replicate the viral genome and proteins. This is, for the...before being translated into proteins. RNA viruses replicate their genomes using a variety of RNA or DNA intermediaries... bacteria and eukaryotes, which use a binary replication process whereby one cell copies itself and “splits...