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We narrowed to 876 results for: lic

Showing: 101 - 120 of 876 results

  1. Cancer Research Plasmids and Resources

    Type
    Collection
    ...signaling network for transitioning between anabolic and catabolic states. Ras Ras is a GTPase that is essential...classification and treatment. On each pathway page, click on a component to find plasmids with your gene of... described in the initial Target Accelerator publications, focused on somatic variants found in lung adenocarcinoma...

  2. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...Origin of Replication? See our Plasmids 101: Origin of Replication blog).  The replication problem Plasmids...directly inhibit replication by binding to the origin of replication and blocking replication machinery (del...cell have the same replicon and/or partitioning system. Let’s start with the replicon- the part of the ...plasmid that contains the origin of replication and the replication control machinery (Need a refresher... with the same replicon are incompatible because they compete for the same replication control machinery... that inhibits replication when copy number in the cell is high, but allows replication when copy number...either indirectly inhibit replication through inhibiting translation of replication machinery proteins, or...

  3. Plasmid Collections

    Type
    Collection
    ...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...together because they are intended for use as a set. Click here to view all kits. Kit Types Fluorescent Proteins... backbone and only differing in a small region. Click here to view all pooled libraries. Pooled Library...plasmids from a depositor organized by a common use. Click here to view all collections. Depositor Collection...

  4. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...Promoter PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to ...non-targeted strand, Cas3 starts unraveling DNA using its helicase properties, cleaving the targeted strand non-specifically...top Plant ID Plasmid Gene/Insert Promoter PI Publication Return to top CRISPR Resources Addgene has a ...

  5. Zhang Lab CRISPR Page

    Type
    Collection
    ... nuclease along with the guide RNA. For most applications, we recommend using Cas9 with the single guide...codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons (Ran et al., Nature Protocols 2013). Individual...SaCas9; SaCas9 sgRNA can be co-transfected as PCR amplicons using a protocol similar to SpCas9 sgRNA PCR (...function of genetic elements. To broadly enable the application of Cas9 in vivo and ex vivo , the Zhang Lab established...can be combined with Cas9 in a wide range of applications. Full references are below. The Cas9 knockin...

  6. Institute for Protein Innovation

    Type
    Collection
    ...Antibody Description Isotype Reactivity Recommended Applications PI Return to top Glypicans Glypicans are a family...Antibody Description Isotype Reactivity Recommended Applications Return to top Integrins Integrins are a family...Antibody Description Isotype Reactivity Recommended Applications Return to top Browse Epitope Tag Antibodies ...Antibody Description Isotype Reactivity Recommended Applications Return to top Browse Antibody Plasmids The following...

  7. Antibody Plasmid Collection

    Type
    Collection
    ...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene...extensively validated for neuroscience research applications from the NeuroMab/Trimmer Lab Recombinant mAb...imaging. Learn more about antibodies and their applications in our Antibody Guide ! Cloning and Expressing...and Generation of synthetic nanobodies against delicate proteins. Nat Protoc. 2020 Apr 8. Markus Seeger...

  8. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Insert Promoter Selectable Marker PI Publication Return to top Drosophila ID Plasmid Gene/Insert Promoter ...Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker...Marker PI Publication Return to top Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable...

  9. Distribution to Industry

    Type
    Collection
    ...characterized (PDB-deposited), disulfide-free, mesophilic, and designed to be diverse in topology, size...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene... Recombinant Antibody Reactivity Recommended Applications PI Don't See What You're Looking For? Please...be processed and shipped. Industry Distribution Policy A portion of Addgene's collection is available ...

  10. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...ori2, ori3) and replication initiation proteins (RepA, RepB) necessary for replication in various hosts...the ori and allow for replication. However, if plasmids have their own replication machinery such as Rep... and W are known to have replicons with broad host ranges. Popular replicons from these groups include...different hosts that a plasmid can transfer to, replicate in, and persist in. Some plasmids have a narrow... while others have a broad host range and can replicate and stably maintain the genes they carry among.... These features are: The plasmid origin of replication (ori) contains structural elements that are versatile...structure allows plasmids to utilize diverse host replication machinery. For instance, the ori typically contains...

  11. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...because devaluing science has alarming implications for our public policies—many of which impact reproductive...science to the public, talking to policy-makers, and broadly defending science in the public sphere as often...Canada.  Outside the lab, she dabbles in science policy, public outreach, and restorative justice.  Follow ...restrictions that conflict with scientific evidence. We need representatives and policymakers who not only ...approach policy makers take in abusing climate science skepticism to promote harmful policies that benefit...move slowly, news about possible broad changes to policies seems to break everyday. Many researchers and ...participation in government, evidence to inform policies, a protected place to work, funding for science...

  12. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...the grant application process. When my PhD advisor first asked me to write a grant application due the ...the next round of applications. Step 5: Keep writing! Each written grant application builds upon the ... project grant application" by the NIH Still want more information on the application process? What types...advisor alone applies for these competitive grant applications; however, in some cases, your advisor may choose...writing, volunteered to take the lead on various applications throughout my graduate career, and helped receive... Step 1: Your PI forwards you a Request For Applications (RFA), what next? First, check the submission...that if a PI asks for assistance in the grant application process, she has faith in you! Additionally, ...

  13. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...in PubMed and choose the appropriate publication. This publication will be listed at the top of the final... with publication details or permission to distribute, or Addgene finds your journal publication describing...6) or you can click the "Save" button and jump around to the different pages by clicking on the appropriate...Add Plasmids page and click the blue "Submit Deposit" button. Figure 13: Click the "Submit Deposit" button... account, you can begin the deposit process by clicking "Submit Online Deposit" (for online submission... menu: Figure 1: Begin the deposit process by clicking "Submit Online Deposit" or "Download Deposit Spreadsheet...submitting plasmid(s) using a spreadsheet (right), click the “Download Deposit Spreadsheet” button to download...

  14. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...are not compatible with regular mesophilic systems are the psychrophilic enzymes.  The reason behind this...weather. Psychrophilic enzymes face very similar issues when forced to express in mesophilic host systems...about placing the mesophilic hosts at 0°C? That won’t work out either - most mesophilic systems would either...mediated editing for psychrophilic systems as well as engineering and regulating psychrophilic recombinant proteins... on a special class of enzymes expressed by psychrophilic (cold-loving) bacteria. These bacteria inhabit...inhabit cold temperature locales and psychrophilic enzymes help them survive under severely cold conditions...with a few basics.  Mesophiles vs. extremophiles Mesophilic organisms are your regular next-door microbes...

  15. Engaging with science and society at pgEd

    Type
    Blog Post
    ...and attending seminars in bioethics, public health, science policy, and science and technology studies ...number of organizations in public engagement efforts, including giving public lectures, writing science...developments in genetics research, their applications and regulatory policies; keeping tabs on the conversation...path to science policy and science communication A career in science communication or policy requires broad...scientists arguably have a duty to engage the public on the implications of their work. Johnny Kung is the Director... space of science education, communication, and policy. In this post, I hope to tell you a little more... as well as the personal, social and ethical implications of genetics. In these conversations, pgEd strives...

  16. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...physiological performance.  2. Trans-splicing Overview: For trans-plicing, splice site donor and acceptor sequences...followed by a splice site donor. The AAV encoding part B of the transgene contains a splice site acceptor...can enhance the rate of trans-splicing (Lai et al., 2005). Trans-splicing does not always outperform single...either the overlapping or the trans-splicing mechanism. The splice site donor and acceptor sequences and...sequence, upstream of the 5’ splice donor and downstream of the 3’ splice acceptor of the transgene to...part B of the transgene form a heterodimer, then splicing of the pre-mRNA of the two halves of the transgene...the full-length transgene.  Drawbacks:  Trans-splicing occurs across the ITR junction, and this is likely...

  17. March for Science

    Type
    Blog Post
    ...and autism, and other hypotheses mount yet policies and public opinion do not seem to take note. For example...to inform the public (8–13). Facing the possibility that reason is losing ground in public discourse, many...Democrats more often than Republicans, the March is not about Democrats or Republicans (16). This is a Pro-...inclusion of science to inform policy (17). The facts surrounding a matter or policy should have more weight ... Idea Pew Research Center: Public Praises Science; Scientists Fault Public, Media March for Science: Principles...when we drop them. Scientific realities limit what policy can do and, in this way, science has always been...what outcomes are actually possible for a given policy. In exchange, the government protects (and often...

  18. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    ...Technical Tips). Minigenome replication and transcription In order to replicate the minigenome, the viral...transcription and replication strategies of Marburg virus and Ebola virus by using artificial replication systems...allowing researchers to study aspects of the EBOV replication cycle under BSL-2 conditions (4). What is a ...that are crucial cis-acting signals for genome replication and viral gene transcription (6). The EBOV genome...5’ ends of the genome which are required for replication and transcription (Figure 1). All viral genes...cis-acting signals necessary to direct minigenome replication and reporter gene transcription by the EBOV polymerase... in 2, 5). The minigenome is capable of being replicated and transcribed in the cell when the appropriate...

  19. 6 Tips for Grant Writing

    Type
    Blog Post
    ...good quality application. Even following this advice, only about 50% of my grant applications have been ...do need to be able to research and write your application, then give it to someone else to read and / or...it). It’s easy for reviewers to spot when an application is rushed (whether the idea isn’t properly researched...researched / developed or the application is full of typos) and these are unlikely to get past the initial...someone else in the department) to read over your application and give you feedback. They’ve most likely been...alterations before submitting - handing them your application 3 days before the deadline isn’t going to be ...problem in a novel way. Finally, engineering new applications to solve problems will provide originality to...

  20. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ...science is all about navigating conflict. In literary terms, conflict can be defined as (1) the struggle...story, you need to have conflict. There are a lot of ways to introduce conflict into your work, but one...problem?” Those are the conflicts that will drive your story forward.  Beyond the Conflict  Once you have your...that the audience can understand the conflict. A science-based conflict gives you an easy framework with ...presence of obstacles that need to be overcome. Conflict, and its inherent tension, is interesting. It’...invested in seeing how they do it.    Finding Conflict in Science  In all science communication, what...talk to a scientist about their research, these conflicts come out quite naturally as they explain their...
Showing: 101 - 120 of 876 results