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We narrowed to 397 results for: nam

Showing: 101 - 120 of 397 results

  1. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...Fluorescent protein based FRET pairs with improved dynamic range for fluorescence lifetime measurements." ... monitor neuronal activity (voltage sensors), to name a few.   Find Many FRET-Based Biosensors on Addgene's...FRET for understanding biomolecular structure and dynamics in live cells." Current opinion in structural ...

  2. Hot Plasmids February 2024

    Type
    Blog Post
    ...triple mutant, Y187F, R144I and T155Q, which they named QC2-6 FIQ or mStayGold (Ando et al., 2023). This...original (Ivorra-Molla et al., 2023). They also named their variant mStayGold. It is worth mentioning ... engineered adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene delivery...

  3. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...Neurons, for example, have unusually fast calcium dynamics, whereas non-neuronal systems tend to have slower... expression (CAG promoter). An improved version named CaMPARI2 is available for neuron-specific expression...calcium indicators to probe complex brain circuit dynamics in vivo. Neuroscience Research. 2021 Aug 1;169...

  4. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ... mK-GO. Large Stokes Shift (LSS): Stokes shift (named after George G. Stokes) is the shift in wavelength...denoted by a “m” as the first letter in the protein name, e.g. mCherry). Oxygen: The maturation of the chromophore...study gene expression, nuclear localization, and dynamics” Curr. Opin. Cell. Biol. 23:310. ...

  5. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...luck, but I’m feeling optimistic for the next tournament). Minus a little rain towards the end, it was...completely new realm. It allows for the usual office dynamics to be mixed up a bit. Who know that the quiet ...also with having a good time. Host a board game tournament or beer hour (only if it’s cool with your legal...

  6. Retrograde AAV viral preps

    Type
    Collection
    ...Inhibitors Other Molecular Tools Clear Filters ID Name Promoter Activity Category PI 37825 AAV-CAG-GFP ...

  7. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...in technical support have? There are different names for technical support roles. Examples are Technical...corners in a company, less exposed to the fast dynamics and pressure which are often found in sales or...assurance, product management, marketing, and sales (to name a few). Depending on the particular company structure...

  8. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...controls are required to make this method useful for dynamic experiments in which FRET changes are large. Two..., but it is destructive and cannot be used for dynamic measurements. Extra care should be taken so as ...donor is shortened, allowing FLIM to give an unambiguous value of FRET efficiency. As you don’t measure...

  9. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...populations over time. The ability to track single cell dynamics at the organism level has been made possible by... the cerebellum of mice, the team appropriately named their technology Brainbow. Brainbow 1.0 used the...multicolor cell labeling systems allow single cell dynamics in a variety of organisms to be observed in more...

  10. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ... There are numerous GFP variants. Here I use the name ‘GFP’, which refers to mEGFP. This variant was made...PMC4927352 7. Hink, Mark A., et al. "Structural Dynamics of Green Fluorescent Protein Fused with a Single...PubMed PMID: 18155467. 13. Chen, Baohui, et al. "Dynamic imaging of genomic loci in living human cells by...

  11. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...MTRIAOT enabled real-time observations of oxytocin dynamics in live mice under a variety of behaviors, opening...real-time measurement of extracellular oxytocin dynamics in the brain. Nat Methods. 2022 Sep 22. pii: 10.1038...means operating at the outer edge of the sensor’s dynamic range. With this in mind, Ed Boyden and colleagues...

  12. Luciferase Plasmid Collection

    Type
    Collection
    ... photons. Its robust signal, sensitivity, wide dynamic range, and resistance to photobleaching have made...

  13. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...Ethan Hawke, Uma Thurman and Jude Law. The movie’s name is itself a combination of the four bases of DNA...involves Langdon chasing an anti-villain geneticist named Zobrist who designs a virus that can potentially...Kartik’s research focuses on the use of molecular dynamics simulations and machine learning to develop a ...

  15. Zhang Lab CRISPR Page

    Type
    Collection
    ...mice, Platt et al . simultaneously modeled the dynamics of KRAS , p53 and LKB1 , the top three significantly...

  17. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Subpool Viral Prep Available Yes No Clear Filters Name ID Library Type Species PI Lentiviral Generation...

  18. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...construct, eUNG-BE4max(R33A)ΔUGI, was given the pithier name CGBE1, for “C → G Base Editor 1,” and had a C →...clear why this is, but it might point to a more dynamic picture of chromatin than an accessible/inaccessible...track of which acronym is which. Acronym Full name Notes CRISPR terms gRNA/sgRNA Guide RNA RNA ...

  19. Biosensor AAV Preps

    Type
    Collection
    ... AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Promoter Sensor Label Activity Serotype PI Acetylcholine...

  20. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...Vivid (VVD, N. crassa) (Kawano et al., 2015). Nicknamed paCas9-1 and consisting of the fusion proteins...photoactivatable CRISPR strategies Publication System nickname Photoactivatable moiety Cas9 variatn CRISPR protein...Phillips-Cremins JE (2019) LADL: light-activated dynamic looping for endogenous gene expression control....
Showing: 101 - 120 of 397 results