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Showing: 101 - 120 of 258 results

  1. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...sequence specific to a virus or pathogen. Once Cas13 recognizes and binds to the programmed sequence, it can ... Cas9 (Zetsche et al., 2015 ). Cas12a however recognizes a different PAM site and generates 5’ and 3’ ...

  2. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...ori typically contains iterons that serve as recognition sites for replication initiation proteins. These...depends on whether the host has the components to recognize the ori and allow for replication. However, if...

  3. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...DNA sequence containing the 38-50 bp sequence recognized by the serine integrase, finding tens of thousands...reverse transcriptases, followed bylanding site recognition and integration of cargo via Cas9-directed integrases...

  4. Transferable Skills Guide: Creativity

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    Blog Post
    ... to fill this gap by both helping researchers recognize their MANY transferable skills learned while working...in which the goal is to generate unique ideas. Recognize ideas can come any time. Meetings shouldn’t be...

  5. CRISPR 101: Any Base Transversion Editors

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    Blog Post
    ... UNG, which excises uracil, could allow it to recognize thymine (T) or cytosine (C). Several different...). In each case, a UNG enzyme was modified to recognize T and/or C substrates. The HuidaGene Therapeutics...

  6. An Inside Look into Addgene's Dev Team

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    Blog Post
    ...from similar backgrounds, we would have similar cognitive biases and this would become apparent in the software... design. Each of us brings slightly different cognitive biases as a result of our diversity which in turn...

  8. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...plasmid between the T-DNA borders (yellow) are recognized by the Vir genes on the helper plasmid and used...with chemical cargoes in plants enabled by a biorecognition motif. Nat Commun 11: . https://doi.org/10.1038...

  9. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...considerations are to make sure your secondary 1) will recognize the species of your primary antibody and 2) is...primary antibody, first, remember that antibodies recognize very specific 3-dimensional epitopes. Different...

  10. Hot Biosensors 2022: Year-End Roundup

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    Blog Post
    ...regions of any G-protein-coupled receptor (GPCR) recognizing a specific ligand (Ino et al. 2022). The oxytocin...more than 20 other MTRIA sensors based on GPCRs recognizing various neurotransmitters, neuropeptides and ...

  12. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...that is methylated at the adenosine of the GATC recognition site. Transformation: After the PCR reaction,...can be achieved using a restriction enzyme that recognizes a unique site in the coding region (preferably...

  15. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...ability of Type IIS enzymes to cut outside their recognition site, and permits DNA fragments with compatible...assembled. Scientists can engineer unique enzyme recognition sites that flank a DNA module in an inverse orientation...

  16. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...directors, we can't celebrate Addgene without recognizing our incredible depositors and requestors. Thank...

  18. Call for Guest Bloggers

    Type
    Blog Post
    ...techniques that simplify and expedite research. We recognize, however, that we can’t do it all ourselves. Therefore...
Showing: 101 - 120 of 258 results