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We narrowed to 266 results for: ogn

Showing: 101 - 120 of 266 results
  1. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...the mutant versions of SpCas9 and SaCas9, which recognize PAM sequences other than NGG (Table-1). Type...or destruction of any restriction enzyme (RE) recognition site, RE analysis of PCR product could be used...
  2. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...optional fluorescence of FPs with facile antibody recognition of epitope tags. Thus they have created antigenic...imaging set-up. In contrast, smFP tags can be recognized by distinct, fluorophore-labeled antibodies with...
  3. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    Blog Post
    ...target DNA (Cpf1 requires only a crRNA for target recognition and cleavage). C2c2 (now referred to as Cas13a... or C base pair. In bacteria, once Cas13a has recognized and cleaved its target RNA sequence as specified...
  4. Plasmids 101: Broad Host Range Plasmids

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    Blog Post
    ...ori typically contains iterons that serve as recognition sites for replication initiation proteins. These...depends on whether the host has the components to recognize the ori and allow for replication. However, if...
  5. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...regions of any G-protein-coupled receptor (GPCR) recognizing a specific ligand (Ino et al. 2022). The oxytocin...more than 20 other MTRIA sensors based on GPCRs recognizing various neurotransmitters, neuropeptides and ...
  6. Antibodies 101: Introduction to Immunofluorescence

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    Blog Post
    ...considerations are to make sure your secondary 1) will recognize the species of your primary antibody and 2) is...primary antibody, first, remember that antibodies recognize very specific 3-dimensional epitopes. Different...
  7. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    Blog Post
    ...plasmid between the T-DNA borders (yellow) are recognized by the Vir genes on the helper plasmid and used...with chemical cargoes in plants enabled by a biorecognition motif. Nat Commun 11: . https://doi.org/10.1038...
  8. Site Directed Mutagenesis by PCR

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    Blog Post
    ...that is methylated at the adenosine of the GATC recognition site. Transformation: After the PCR reaction,...can be achieved using a restriction enzyme that recognizes a unique site in the coding region (preferably...
  9. 22 Hot Plasmid Technologies from 2014

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    Blog Post
    ...ability of Type IIS enzymes to cut outside their recognition site, and permits DNA fragments with compatible...assembled. Scientists can engineer unique enzyme recognition sites that flank a DNA module in an inverse orientation...
  10. Addgene's 20th Anniversary Party

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    Blog Post
    ...directors, we can't celebrate Addgene without recognizing our incredible depositors and requestors. Thank...
  11. Call for Guest Bloggers

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    Blog Post
    ...techniques that simplify and expedite research. We recognize, however, that we can’t do it all ourselves. Therefore...
Showing: 101 - 120 of 266 results