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We narrowed to 252 results for: p

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Showing: 101 - 120 of 252 results

  1. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ... effectively able to introduce point mutations in P. aeruginosa, Pseudomonas putida, Pseudomonas fluorescens...doi.org/10.1038/nbt.3043 Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA (2013) Programmable...10.1038/nbt.2508 Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar S, Davies BW, Ellington...10.1128/mbio.00019-20 Singh AK, Carette X, Potluri L-P, Sharp JD, Xu R, Prisic S, Husson RN (2016) Investigating...

  2. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ..., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., & Zhang...//doi.org/10.1128/mcb.18.1.93  Ran, F. A., Hsu, P. D., Lin, C., Gootenberg, J. S., Konermann, S., Trevino...doi.org/10.1016/j.cell.2013.08.021  Ran, F. A., Hsu, P. D., Wright, J., Agarwala, V., Scott, D. A., & Zhang...., Meng, X., Urnov, F. D., Rebar, E. J., Gregory, P. D., Zhang, H. S., & Jaenisch, R. (2011). Generation...

  3. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...genes that affect autophagy (ATG) in yeast. Atg8p (“p” stands for protein) associates with autophagosomal...PMC2852113. 4. Klionsky, D. J., Cuervo, A. M., & Seglen, P. O. (2007). Methods for Monitoring Autophagy from ...

  4. RNA Extraction Without A Kit

    Type
    Blog Post
    .... https://doi.org/10.1021/bi00591a005 Chomczynski P, Sacchi N (1987) Single-Step Method of RNA Isolation.../doi.org/10.1016/0003-2697(87)90021-2 Chomczynski P, Sacchi N (2006) The single-step method of RNA isolation...

  6. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ... immunity to viral invasion.  His lab is studying P. furiosus, a hyperthermophile that exists in sea vents...regions of the prokaryotic genome. Then he desribed how P. furiosus has systems to defend against both DNA and...

  8. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...pEVOL-pAzF synthetase for p-azido-l-phenylalanine E. coli pEVOL-pBpF synthetase for p-benzoyl-l-phenylalanine...383. PubMed PMID: 12605205. 13. Yan, Qi, and Marcel P. Bruchez. "Advances in chemical labeling of proteins...

  9. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ... ER-negative HEK293 cells." PLoS One, 2014. 9(6): p. e98635. PubMed PMID: 24901218. PubMed Central PMCID...2015): 2321-2332. 3. Contamination, Ileana S., John P. S., Basic Methods, Human Stem Cell Manual (Second...

  10. Common Injection Routes in Mice

    Type
    Blog Post
    ... plasmids Cre-Lox plasmids References Prathipati, P., Rodriguez-Aguayo, C., Walton, B. L., Sood, A. K.....), The Laboratory Mouse. Academic Press. Turner, P. V., Brabb, T., Pekow, C., & Vasbinder, M. A. (2011...

  11. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...Therefore, researchers must co-transfect cells with 5’-P-ssDNA guides and an NgAgo expression plasmid to edit...endonuclease) protein that can form a complex with a 5’-P-ssDNA guide at room temperature. Until that time, ...

  12. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...research   Optimized prime editing in dicots By Emily P. Bentley Prime editing (PE), initially developed in... H., Natale, S., Gomez, L., Taxidis, J., O'Neill, P. S., Villette, V., Bradley, J., Wang, Z., Jiang, D...

  13. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...Libraries here! Singh, I., Fernandez-Perez, D., Sanchez, P. S., & Rodriguez-Fraticelli, A. E. (2025). Pre-existing...ATLAS for anterograde synaptic connections By Emily P. Bentley While several viral tools can perform neuronal...

  14. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ..., Public Library of Science (PLoS), 18 Feb. 2011, p. e16765. Crossref, https://doi.org/10.1371/journal.pone...5, Public Library of Science (PLoS), 30 May 2018, p. e0197185. Crossref, https://doi.org/10.1371/journal.pone...Communications, vol. 2, no. 2, Elsevier BV, Mar. 2021, p. 100135. Crossref, https://doi.org/10.1016/j.xplc.2020.100135...

  15. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...membrane stacked behind the p membrane. If your protein runs through the p membrane into the psi membrane...membrane for small proteins (< 15 kDa) and a 0.45 µm (p) membrane for larger ones. Changing your membrane ...membrane, that’s a good indication that the p membrane pores are too large.  Transfer method   Wet transfers...

  18. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...potential to induce chromosomal aberrations. Pfeiffer P, et al. Mutagenesis (2000). PubMed. Multiple Pathways...Geneticist's practical guide to CRISPR applications. Singh P, et al. Genetics 114.169771; (2014). PubMed. The...

  19. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...Niehaus in December 2015 and was updated by Emily P. Bentley in June 2024.  References and resources ...J. E., Rozgaja, T. A., Gupta, R., Dyson, K., Rao, P., & Leonard, A. C. (2018). Origin recognition is the...
Showing: 101 - 120 of 252 results