Celebrating the 2024 Nobel Prize in Chemistry
Type
Blog Post
... Emily P. Bentley...
We narrowed to 241 results for: p
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Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog Post...pEVOL-pAzF synthetase for p-azido-l-phenylalanine E. coli pEVOL-pBpF synthetase for p-benzoyl-l-phenylalanine...383. PubMed PMID: 12605205. 13. Yan, Qi, and Marcel P. Bruchez. "Advances in chemical labeling of proteins... -
10 Basic tips for mammalian cell culture
TypeBlog Post... ER-negative HEK293 cells." PLoS One, 2014. 9(6): p. e98635. PubMed PMID: 24901218. PubMed Central PMCID...2015): 2321-2332. 3. Contamination, Ileana S., John P. S., Basic Methods, Human Stem Cell Manual (Second... -
Common Injection Routes in Mice
TypeBlog Post... plasmids Cre-Lox plasmids References Prathipati, P., Rodriguez-Aguayo, C., Walton, B. L., Sood, A. K.....), The Laboratory Mouse. Academic Press. Turner, P. V., Brabb, T., Pekow, C., & Vasbinder, M. A. (2011... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog Post...Therefore, researchers must co-transfect cells with 5’-P-ssDNA guides and an NgAgo expression plasmid to edit...endonuclease) protein that can form a complex with a 5’-P-ssDNA guide at room temperature. Until that time, ... -
Hot Plasmids: Summer 2025
TypeBlog Post...Libraries here! Singh, I., Fernandez-Perez, D., Sanchez, P. S., & Rodriguez-Fraticelli, A. E. (2025). Pre-existing...ATLAS for anterograde synaptic connections By Emily P. Bentley While several viral tools can perform neuronal... -
Hot Plasmids: Winter 2025
TypeBlog Post...research Optimized prime editing in dicots By Emily P. Bentley Prime editing (PE), initially developed in... H., Natale, S., Gomez, L., Taxidis, J., O'Neill, P. S., Villette, V., Bradley, J., Wang, Z., Jiang, D... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog Post..., Public Library of Science (PLoS), 18 Feb. 2011, p. e16765. Crossref, https://doi.org/10.1371/journal.pone...5, Public Library of Science (PLoS), 30 May 2018, p. e0197185. Crossref, https://doi.org/10.1371/journal.pone...Communications, vol. 2, no. 2, Elsevier BV, Mar. 2021, p. 100135. Crossref, https://doi.org/10.1016/j.xplc.2020.100135... -
Troubleshooting and Optimizing a Western Blot
TypeBlog Post...membrane stacked behind the p membrane. If your protein runs through the p membrane into the psi membrane...membrane for small proteins (< 15 kDa) and a 0.45 µm (p) membrane for larger ones. Changing your membrane ...membrane, that’s a good indication that the p membrane pores are too large. Transfer method Wet transfers... -
Performing In Vivo CRISPR Screens Using the FITS Approach
TypeBlog Post...Hickok, G. H., Lemmen, A. M., Nguyen, T. H., Prathima, P., Fithian, W., Schwartz, M. A., Hacohen, N., Doench... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...Christiaen p(U6a-BsaI-gRNA) 65955 Other/Tribolium castaneum BsaI none S. pyogenes Averof p(U6b-BsaI-gRNA... -
CRISPR 101: Homology Directed Repair
TypeBlog Post...potential to induce chromosomal aberrations. Pfeiffer P, et al. Mutagenesis (2000). PubMed. Multiple Pathways...Geneticist's practical guide to CRISPR applications. Singh P, et al. Genetics 114.169771; (2014). PubMed. The... -
QC Sequencing Technologies at Addgene
TypeBlog Post... References Niedringhaus, T. P., Milanova, D., Kerby, M. B., Snyder, M. P., & Barron, A. E. (2011). Landscape... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog Post...Niehaus in December 2015 and was updated by Emily P. Bentley in June 2024. References and resources ...J. E., Rozgaja, T. A., Gupta, R., Dyson, K., Rao, P., & Leonard, A. C. (2018). Origin recognition is the... -
Five Popular Model Organisms, Part 2
TypeBlog Post.... https://doi.org/10.1038/nature02426 Iannaccone, P. M., & Jacob, H. J. (2009). Rats! Disease Models &...Liu, L. S., Zhao, L. Y., Wang, S. H., & Jiang, J. P. (2016). Research proceedings on amphibian model organisms... -
Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog Post...Gootenberg JS, Abudayyeh OO, Lee JW, Essletzbichler P, Dy AJ, Joung J, Verdine V, Donghia N, Daringer NM...Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin... -
"Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science
TypeBlog Post..., S., Amster, A., Arbuckle, J., Ayala, A., Baker, P. M., . . . Tasic, B. (2025). A suite of enhancer AAVs...., Fong, O., Yao, S., Mortrud, M., . . . Levi, B. P. (2021). Functional enhancer elements drive subclass-selective... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog Post...! References and Resources References Bartel, D. P. (2004). MicroRNAs. Cell, 116(2), 281–297. https:/... D., Abugessaisa, I., Alam, T., Arner, E., Arner, P., Ashoor, H., Åström, G., Babina, M., Bertin, N., ... -
CRISPR 101: Any Base Transversion Editors
TypeBlog Post..., Zhang, D., Zhang, S., Li, C., Wu, J., Randolph, P. B., Sousa, A. A., Qu, C., Zhu, Y., Guan, Y., Wang... Emily P. Bentley... -
A Control for All Seasons
TypeBlog Post...were run on a western against a protein standard (P) to check for tau molecular weight shifts. A ...PubMed Central PMCID: PMC4212362. Alexander, Steve P. H., et al. “Goals and Practicalities of Immunoblotting...