...membrane stacked behind the p membrane. If your protein runs through the p membrane into the psi membrane...membrane for small proteins (< 15 kDa) and a 0.45 µm (p) membrane for larger ones. Changing your membrane ...membrane, that’s a good indication that the p membrane pores are too large.  Transfer method   Wet transfers...

...Hickok, G. H., Lemmen, A. M., Nguyen, T. H., Prathima, P., Fithian, W., Schwartz, M. A., Hacohen, N., Doench...

...Niehaus in December 2015 and was updated by Emily P. Bentley in June 2024.  References and resources ...J. E., Rozgaja, T. A., Gupta, R., Dyson, K., Rao, P., & Leonard, A. C. (2018). Origin recognition is the...

...potential to induce chromosomal aberrations. Pfeiffer P, et al. Mutagenesis (2000). PubMed. Multiple Pathways...Geneticist's practical guide to CRISPR applications. Singh P, et al. Genetics 114.169771; (2014). PubMed. The...

...Christiaen p(U6a-BsaI-gRNA) 65955 Other/Tribolium castaneum BsaI none S. pyogenes Averof p(U6b-BsaI-gRNA...

...Gootenberg JS, Abudayyeh OO, Lee JW, Essletzbichler P, Dy AJ, Joung J, Verdine V, Donghia N, Daringer NM...Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin...

.... https://doi.org/10.1038/nature02426 Iannaccone, P. M., & Jacob, H. J. (2009). Rats! Disease Models &...Liu, L. S., Zhao, L. Y., Wang, S. H., & Jiang, J. P. (2016). Research proceedings on amphibian model organisms...

..., Zhang, D., Zhang, S., Li, C., Wu, J., Randolph, P. B., Sousa, A. A., Qu, C., Zhu, Y., Guan, Y., Wang... Emily P. Bentley...

...were run on a western against a protein standard (P) to check for tau molecular weight shifts. A ...PubMed Central PMCID: PMC4212362. Alexander, Steve P. H., et al. “Goals and Practicalities of Immunoblotting...

...incorporated into the full-length protein. Up to 80% p-Ser incorporation into E17TAG GFP has been achieved...incorporation due to differences in stability of the p-Ser incorporated protein as well as context-specific...

...subdivide neural expression patterns. Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH. Nature...with combinatorial labels. Loulier K, Barry R, Mahou P, Le Franc Y, Supatto W, Matho KS, Ieng S, Fouquet ...

...Use active listening skills such as the PAC Method. P = demonstrate patience, pause; A = ask at least one...

...Zuris, J. A., Thompson, D. B., Shu, Y., Guilinger, J. P., Bessen, J. L., Hu, J. H., Maeder, M. L., Joung, ...

...doi.org/10.1038/s41477-020-0704-5 Santana I, Wu H, Hu P, Giraldo JP (2020) Targeted delivery of nanomaterials...doi.org/10.1038/s41467-020-15731-w Wang F, Wang C, Liu P, Lei C, Hao W, Gao Y, Liu Y-G, Zhao K (2016) Enhanced...

... References and Resources References Andrews, N. P., Boeckman, J. X., Manning, C. F., Nguyen, J. T., ...protocols.io.bs68nhhw Im, K., Mareninov, S., Diaz, M. F. P., & Yong, W. H. (2019). An Introduction to Performing...

..., De Los Angeles A, Mali P, Aach J, Kim-Kiselak C, Briggs AW, Rios X, Huang P-Y, Daley G, Church G (2013...

...Staber, S. J. Wheelan, J. K. Joung, P. B. McCray, F. D. Bushman, P. L. Sinn, and N. L. Craig, “piggyBac...

...producing difficult-to-fold proteins. In addition, P. pastoris has the capacity to glycosylate proteins...

...Bandrowski, A., Trimmer, J. S., Rego, M., Freedman, L. P., Ferrara, F., Bradbury, A. R. M., Cable, H., & Longworth...

...loci in human cells. Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. Proc Natl Acad Sci...