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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 303 results for: rigi

Showing: 101 - 120 of 303 results

  1. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...modifications listed can refer to changes made to the original backbone by the depositing lab, or by a previous...previously deposited with Addgene, you can list the original vector’s plasmid number here. Gene/insert: This...

  3. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ... to community colleges, and has helped provide original research experiences for thousands of undergraduates...cytosolic MDH (hMDH1). They then expressed the original and recombinant enzymes in bacteria, purified ...

  6. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ... [IPI-SV5-Pk1] is a recombinant version of the original V5 tag mouse monoclonal antibody, which recognizes...4 protein, mScarlet3 was 50% brighter than the original worm-optimized wrmScarlet. mScarlet-I3, while ...

  7. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...fold improvement in detection efficiency over the original CROPseq. Find CROPseq-multi plasmids here Walton...2021). Check out their paper for details on the original antibodies. Find Anti-SARS-CoV-2 Antibodies here...

  8. How to Write a Scientific Review Article

    Type
    Blog Post
    ...citations so that people can go back and read the original reference for the data. For example, we summarized...how big the studies were, and you could find the original paper if you wanted to learn more. Another example...

  9. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...Addgene, you’ll usually receive an aliquot from the original library constructed by the depositing lab. (For... library should be very similar to that of the original library. Like amplification, this step also depends...

  10. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ...cross-contamination, we recommend retrieving plasmids from your original Addgene kit plate as few times as possible and...gets contaminated, you can resort back to your original Addgene kit plate for fresh bacteria. Performing...

  11. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ... shown in Figure 2, this was likely due to the original transfection conditions. Now that we have successfully...PMCID: PMC3321166. Resources on the Addgene Blog Origin of Replication The Promoter Region – Let's Go! ...

  13. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...into two groups. Seven are reconstructions of the original hybridomas as new reproducible rat or mouse rAbs.... Based on the established applications of the original hybridoma monoclonals, we anticipate that our ...

  14. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...duplications (slightly larger bands sizes relative to the original template). Due to the small difference in size...mutated product can then be cloned back into the original plasmid using the unique restriction sites. Alternatively...

  15. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...phosphoserine-incorporation mutant for a residue that wasn’t originally a serine (e.g. mutating a threonine residue to... @nieminm.   References 1. Cohen, Philip. "The origins of protein phosphorylation." Nature cell biology4.5...

  16. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...cloning and maintenance of a plasmids with R6Kγ origin; contains a mutant allele of the pir gene that ...organisms! Our Most Popular Plasmids 101 Posts: Origin of Replication The Promoter Region – Let's Go! ...

  18. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...Originally published May 3, 2017 and last updated Sep 24, 2020 This post was contributed by guest blogger...control, one should revert the edit back to the original in order to prove that the phenotype was really...

  19. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...et al., 2009). Since multimers have additional origins of replication compared to monomers, they are also...  Plasmids 101: What is a plasmid? Plasmids 101: Origin of ReplicationPlasmids 101: PCR...

  20. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...receptor binding protein hit and can go back to the original plate to identify which phage gene encodes that...colony expressing a functional binding protein was originally located. Now you can grow up your colonies of...
Showing: 101 - 120 of 303 results