We narrowed to 129 results for: sacs

1. Tips for Screening with Yeast Two Hybrid Systems

   Type

   Blog Post

   ...Two hybrid systems were developed in Saccharomyces cerevisiae in 1989 and are still used extensively ...

2. Cpf1: A New Tool for CRISPR Genome Editing

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   Blog Post

   ...that of the widely used SpCas9 and comparable to SaCas9. As seen with Cas9 orthologs, Cpf1 efficiency varies...

3. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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   Blog Post

   ... only game in town, and other Cas proteins like SaCas9 and Cpf1 can circumvent the limitations associated...

4. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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   Blog Post

   ...So, think beyond Drosophila melanogaster or Saccharomyces cerevisiae (both fine research organisms) -  ...

5. Supporting Reproducibility with a Connected ELN

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   Blog Post

   ...electronic lab notebook. Rory is based in Cambridge, Massachusetts, and feels privileged to be part of the vibrant...

6. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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   Blog Post

   ...Chicanos/Hispanics and Native Americans in Science’s (SACNAS) annual conference all foster mentorship in different...

7. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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   Blog Post

   ...Sadowski. 1985. “The FLP Recombinase of the Saccharomyces Cerevisiae 2 Micron Plasmid Attaches Covalently...

8. Plasmids 101: The Promoter Region – Let's Go!

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   Blog Post

   ...can be turned on or off depending on what tet transactivator is used. UAS General expression mRNA Yeast...

9. CRISPR 101: Homology Directed Repair

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   Blog Post

   ...Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae. Paques F and Haber JE. Microbiology...

10. Filming Science Videos in the Age of Social Distancing

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    Blog Post

    ...Forward Design: We produced a video celebrating Massachusetts Nonprofit Day, which was the perfect opportunity...

11. Treating Muscular Dystrophy with CRISPR Gene Editing

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    Blog Post

    ... et al. and Tabebordbar et al. used the shorter SaCas9 with AAV8 and AAV9, respectively. Although each...

12. How to Negotiate a Successful Lab Start Up Budget

    Type

    Blog Post

    ...early investigators making unnecessary short term sacrifices that hurt their goals, rather than focusing on...

13. How to Design Your gRNA for CRISPR Genome Editing

    Type

    Blog Post

    ...most widely-used Cas enzyme, the development of SaCas9, NmeCas9, Cas12a enzymes, and engineered variants...

14. Viral Vectors 101: AAV Variables That Matter

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    Blog Post

    ...Response against the Tetracycline-Dependent Transactivator Correlates with Long-Term Doxycycline-Regulated...

15. Building Global Connections with the International Mentorship Program USA-EUROPE

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    Blog Post

    ...Universities in USA and Europe (Harvard University, Massachusetts Institute of Technology, Boston University, ...

16. Quick Guide to All Things Lentivirus

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    Blog Post

    ...such as Doxycycline (Dox), the Tet-controlled transactivator (tTA, also expressed by your transfer vector...

17. A Guide to Getting Started in Undergrad Research

    Type

    Blog Post

    ...presenting your research at conferences such as SACNAS and ABRCMS. These conferences have a lot of programming...

18. CRISPR History and Development for Genome Engineering

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    Collection

    ...also been characterized. Staphylococcus aureus (SaCas9) is smaller than SpCas9 and more easily packaged...

19. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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    Blog Post

    ...protein‐tracking studies in the budding yeast Saccharomyces cerevisiae." Yeast 25.9 (2008): 651-659. PubMed...

20. Plasmids 101: Modular Cloning Applications and Kits

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    Blog Post

    ...Toolkit for Extensive and Flexible Engineering of Saccharomyces Cerevisiae.” ACS Synthetic Biology, vol. 12,...