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We narrowed to 326 results for: sars

Showing: 101 - 120 of 326 results
  1. Components of CRISPR/Cas9

    Type
    Blog Post
    ...This individual protein has all the components necessary to: 1. Bind to a guide RNA The guide RNA, which...specific genomic locus and the scaffolding sequence necessary for Cas9 binding. When using CRISPR/Cas9 for genome...
  2. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...Neomycin selection.   Table 1: Gamma-retrovirus glossary Plasmid Type Element Purpose Envelope... 3' ends of the provirus). Contains sequences necessary for activation of viral genomic RNA transcription...
  3. Antibodies 101: ChIP

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    Blog Post
    ...until you want to break them. Crosslinking is necessary for most ChIP experiences, but the increased binding...quite strongly to DNA, crosslinking may not be necessary. This is known as Native ChIP.  If you’re working...
  4. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...plasmids used in the lab typically contain all the necessary components to allow shuttling between E. coli ...transformation efficiency; however, this region does not necessarily promote replication.  Selection markers for yeast...
  5. Summer SciComm Series: Cognitive Load

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    Blog Post
    ...surface, but it’s quite easy to add in “necessary” detail after “necessary” detail and end up completely overwhelming...
  6. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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    Blog Post
    ...targeted autophagy of mitochondria. As shown in Sargsyan et al. It is also possible to make Rosella fusions...doi:10.4161/auto.5331. PubMed PMID: 18094608 2. Sargsyan, A., Cai, J., Fandino, L. B., Labasky, M. E., ...
  7. Plasmids 101: The Wide World of Natural Plasmids

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    Blog Post
    ... toxins like colicin typically also carry the necessary resistance genes to help cells survive their own...cell, but this functional organization doesn’t necessarily reflect genetic relationships or plasmid incompatibility...
  8. Viral Vectors 101: Calcium Sensors

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    Blog Post
    ...calcium binding sites per sensor (to decrease unnecessary calcium buffering and to increase sensitivity...great FRET sensor option if you have the setup necessary for readout.   We’ve got a live one ...
  9. Rabies and Neuronal Tracing

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    Blog Post
    ...into viral genome, so viral progeny will not necessarily express EnvA unless it is supplied in trans. ...phenomenon has shown that the RABV G protein is necessary and sufficient for retrograde transmission (Etessami...
  10. Adenovirus Guide

    Type
    Guide
    ...2021). Adenoviral vector vaccine platforms in the SARS-CoV-2 pandemic . NPJ Vaccines, 6 (1), 97. https:... Common Uses Safety Resources Plasmid Elements Glossary Credits Adenoviruses (AdV) are medium-sized, non-enveloped...virus enters the host cell, and encode proteins necessary for viral DNA replication and host cell manipulation...vectors or simply need a refresher, we've included a glossary at the end! Browse Adenoviral Plasmids Adenovirus... containing the rest of the adenoviral genome necessary for viral production and homology regions for ...containing the ccdB gene, a ccdB-resistant strain is necessary. For more information on cloning and working with...e.g. pAdEasy) — containing the adenoviral genes necessary for viral production. In AdEasy™, the transgene...
  11. Viral Vectors 101: Voltage Indicators

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    Blog Post
    ...with these sensors, but further optimization is necessary for these to be as effective at the single cell...perform poorly in two-photon conditions, which are necessary conditions for penetrating deeper tissues depths...
  12. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ... have the power to take the added precautions necessary to keep your cells from becoming contaminated....you’re wearing before you start. Also, avoid unnecessary talking because talking generates microbe-laden...
  13. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...content.  What are you interested in? Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com... of a learning curve it will be to get to the necessary level  of proficiency.  What kind of content are...
Showing: 101 - 120 of 326 results