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  1. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...planning, and funding – they may not be right or necessary for many scientists! Unless some of these logistics...housing, transportation, living expenses, and necessary reagents. Your primary lab may be willing to cover...

  2. Components of CRISPR/Cas9

    Type
    Blog Post
    ...This individual protein has all the components necessary to: 1. Bind to a guide RNA The guide RNA, which...specific genomic locus and the scaffolding sequence necessary for Cas9 binding. When using CRISPR/Cas9 for genome...

  4. Antibodies 101: ChIP

    Type
    Blog Post
    ...until you want to break them. Crosslinking is necessary for most ChIP experiences, but the increased binding...quite strongly to DNA, crosslinking may not be necessary. This is known as Native ChIP.  If you’re working...

  7. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...Neomycin selection.   Table 1: Gamma-retrovirus glossary Plasmid Type Element Purpose Envelope... 3' ends of the provirus). Contains sequences necessary for activation of viral genomic RNA transcription...

  8. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...plasmids used in the lab typically contain all the necessary components to allow shuttling between E. coli ...transformation efficiency; however, this region does not necessarily promote replication.  Selection markers for yeast...

  9. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...surface, but it’s quite easy to add in “necessary” detail after “necessary” detail and end up completely overwhelming...

  11. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...targeted autophagy of mitochondria. As shown in Sargsyan et al. It is also possible to make Rosella fusions...doi:10.4161/auto.5331. PubMed PMID: 18094608 2. Sargsyan, A., Cai, J., Fandino, L. B., Labasky, M. E., ...

  13. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...into viral genome, so viral progeny will not necessarily express EnvA unless it is supplied in trans. ...phenomenon has shown that the RABV G protein is necessary and sufficient for retrograde transmission (Etessami...

  14. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...calcium binding sites per sensor (to decrease unnecessary calcium buffering and to increase sensitivity...great FRET sensor option if you have the setup necessary for readout.   We’ve got a live one ...

  15. Plasmids 101: The Wide World of Natural Plasmids

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    Blog Post
    ... toxins like colicin typically also carry the necessary resistance genes to help cells survive their own...cell, but this functional organization doesn’t necessarily reflect genetic relationships or plasmid incompatibility...

  17. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ... have the power to take the added precautions necessary to keep your cells from becoming contaminated....you’re wearing before you start. Also, avoid unnecessary talking because talking generates microbe-laden...

  18. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...with these sensors, but further optimization is necessary for these to be as effective at the single cell...perform poorly in two-photon conditions, which are necessary conditions for penetrating deeper tissues depths...

  20. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...content.  What are you interested in? Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com... of a learning curve it will be to get to the necessary level  of proficiency.  What kind of content are...
Showing: 101 - 120 of 324 results