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TypeBlog Post...volunteers as well. 4) Blog - Hone your writing skills Take the DIY approach and start a blog. Stellar...outreach, it also helps you to hone your writing skills and make a name for yourself. Pick a topic that...institutions and the Royal Society gives training in media skills, writing about your research and residential communication...
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6 Tips for Grant Writing
TypeBlog Post...demands attention, practice, and honing of your skills from the start. Just like with any sport, hours...communicator More and more fellowship applications are asking for a 200-300 word explanation of your research...Some Ways to Improve Your Science Communication Skills 5. Practice makes perfect, apply for small grants... -
How-to: 5 Steps to a Great Panel Discussion
TypeBlog Post... a really lively discussion, I strongly suggest asking the panelists not to use any slides for the event... if you have to, but this is tough for women in skirts, especially short people – which I am, so I hate...areas of science or expertise by listing some and asking people to raise hands in response. I am always ... -
How to Be an Excellent Trainee
TypeBlog Post...articles on building successful mentor relationships, asking for mentor support, and choosing your mentor. What...the moment….but you’ll probably forget and end up asking your mentor later if you don’t write it down. And..., What Now? Will You Be My Mentor? Finding and Asking for Mentoring Support ... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog Post... might enjoy doing it. I’ll also talk about the skills you’ll need to do well as a healthcare consultant... experts. Hence, active honing of communication skills is a must to be successful in this field. Personally... in reviewing literature, and critical analyses skills were applicable to my cases. I can directly use... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog Post...translational complex assembly. PhD student Dejan Gagoski used SITS to create the pCell-Free vectors, Gateway-compatible...both prokaryotic and eukaryotic cell extracts (Gagoski et al 2015). He has also constructed a library ...systems: E. coli, wheat germ, HeLa, and Leishmania (Gagoski, et al 2015[2]). The proteins encoded in this set... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog Post...mentored advanced training to enhance the professional skills and research independence needed to pursue his ...and should be able to take steps to broaden their skills beyond those required at the lab bench. At Addgene...university publication, improving your general writing skills will help you in your academic publishing goals... -
Important Considerations When Using AAVs
TypeBlog Post.... So how do you choose the right serotype? Keep asking questions! Table 1: AAV Serotypes and Tropism ...McCarty, D. M., Paul E. Monahan, and Richard J. Samulski. "Self-complementary recombinant adeno-associated...PMC190186. 9. Grieger, Joshua C., and Richard J. Samulski. "Packaging capacity of adeno-associated virus... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog Post...recognition sites (i.e., loxP and FRT, respectively) (Abremski and Hoess, 1984; Van Duyne, 2001; Christenson ...PMID: 29743652. PubMed Central PMCID: PMC5943452. Abremski, Ken, and Ronald Hoess. "Bacteriophage P1 site-specific.... Hirsch, Matthew L., Sonya J. Wolf, and R. J. Samulski. "Delivering transgenic DNA exceeding the carrying... -
Protocol - How to Purify DNA from an Agarose Gel
TypeProtocol...contaminating your sample. To accomplish this, it is best to skip lanes between samples and between the ladder and... -
Typing CRISPR Systems
TypeBlog Post...10.1038/nature24049 Altae-Tran, H., Kannan, S., Suberski, A. J., Mears, K. S., Demircioglu, F. E., Moeller...6673). https://doi.org/10.1126/science.adi1910 Chylinski, K., Makarova, K. S., Charpentier, E., & Koonin...://doi.org/10.1089/crispr.2021.0051 Özcan, A., Krajeski, R., Ioannidi, E., Lee, B., Gardner, A., Makarova... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog Post...tissues, including lymph nodes, brain, liver, gut and skin. Two-photon intravital microscopy. The video above... cells and follicular dendritic cells within the skin and lymph node respectively. The permanent labelling...the cross-presenting dendritic cell subsets in the skin-draining lymph node." Proceedings of the National... -
Video Library
TypeProtocol...Aseptic Technique Aseptic technique is a critical skill for success in numerous fields such as microbiology... -
Lab Safety for Biosafety Levels One and Two
TypeProtocol...container. Sharps include anything that can pierce your skin, including, but not limited to broken glass or needles... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog Post...blue light is readily absorbed and scattered by the skin, so it is not able to reach deeper tissues. Additionally... however, can penetrate 5 mm past the surface of skin and is less phototoxic. Using the FAST system, Ye...spatial control of Cas9 cleavage when using a photomasking technique. Last, Hemphill et al. presented data... -
Using AAV for Neuronal Tracing
TypeBlog Post... E. R. 2nd, Kadoya, K., Hirsch, M., Samulski, R. J., and Tuszynski, M. H. (2008). Efficient retrograde...PMCID: PMC5529245. Choi, V.W., McCarty, D.M., and Samulski, R.J. (2005). AAV Hybrid Serotypes: Improved Vectors... -
Using a Light Microscope Protocol
TypeProtocol...do not and you may have to dust off your drawing skills. Regardless of your image capture method, be sure... -
Streaking for Single Colonies: The Streak Plate Challenge
TypeBlog Post...colonies is an integral part of any bacteriologist’s skill set. So when Dave Westenberg taught this concept...Want to level up your or your class’s streak plate skills? Find the chromoprotein expressing plasmids here... -
Troubleshooting and Optimizing a Western Blot
TypeBlog Post... seeing a weird pattern in your background, try asking more experienced labmates or looking it up online...ResearchGate’s western blot forum. Blocking Don’t skimp on the blocking time! Fill the time with smaller...detection reactions. Like blocking, you don’t want to skimp on your washing steps! It’s important to let them... -
A Conference By Postdocs For Postdocs: Future of Research
TypeBlog Post...insufficient (or no) emphasis on developing transferable skills to enable pursuit of these diverse opportunities...discussed, the organizers are building valuable skills and meeting new people by working together on the...