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We narrowed to 361 results for: tac

Showing: 101 - 120 of 361 results
  1. FPbase: A new community-editable fluorescent protein database

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    Blog Post
    ...with a valid email address).  One may also simply attach important references that serve to further characterize...much appreciate feedback, so try out the site and contact us if you have any ideas or requests.  Or, if you...
  2. R Bodies: Membrane-Rupturing Microscopic Tools

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    Blog Post
    ...with a fluorescent protein remain functional. Attaching the fluorescent protein fusions allowed us to ...the bacteria apart; however, they still remain attached to the ciliate. It’s thought that they function...
  3. Giving gRNAs a Facelift - Synthetic and Beyond

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    Blog Post
    ... protein. These RNA molecules are vulnerable to attack by both cytoplasmic and nuclear nucleases, as they...group of the ribose to protect it from nucleolytic attack. Another stabilizing sugar modification, found ...
  4. Antibodies 101: ChIP

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    Blog Post
    ...separate them. Most proteins are not likely to remain attached to the DNA throughout the sample processing and...sample may need more or less cross-linking to firmly attach the proteins to the DNA, depending on how tightly...
  5. Lighting Up Cell Signaling with Photoswitchable Kinases

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    Blog Post
    ... used to turn “on” and “off” MEK1 activity by attaching two dimerizing photoswitchable Dronpa (dron= a...at sites homologous to the locations they were attached to in MEK. The photoswitchable versions of MEK1...
  6. Seven Tips for Using LinkedIn as a Scientist

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    Blog Post
    ... look at their profile. Make your professional contact information available. When I am speaking at a ...touch if you have business or follow up. Use the "Contact info" link right below their photo. 2) Personalize...
  7. RNA Extraction Without A Kit

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    Blog Post
    ... lysis, RNA is degraded. To effectively isolate intact RNA, a fast, strong protein denaturant would be...ultracentrifuged for up to 20 hours to separate the intact RNA from DNA and proteins. Although very effective...
  8. Antibodies 101: Secondary Antibodies

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    Blog Post
    ...method relies on multiple secondary antibodies attaching to a single primary antibody, it is sometimes ...reality, multiple primary antibodies can and do attach to a single target protein, so this assumption ...
  9. Hot Plasmids and Viral Preps - January 2021

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    Blog Post
    ... for a knock-out in your experiments? AAV-flex-taCasp3-TEVp from Nirao Shah's and Jim Well's lab induces...use viral prep in serotype AAV5. Find AAV-flex-taCasp3-TEVp here The red-shifted channelrhodopsin (C1V1...
  10. Hot Plasmids - October 2020

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    Blog Post
    ... elegans cell lines to express wrmScarlet1-10 unattached to any other cellular protein. They used CRISPR...Find the lentiviral preps at Addgene hSyn1-SIO-stGtACR1-FusionRed is a Cre-dependent, soma-targeting inhibitory...
  11. Plasmids 101: Modular Cloning

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    Blog Post
    ...another optimized platform. Gene Stacking As the name implies, gene stacking is the use of a combination of...
  12. CRISPR in the Clinic

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    Blog Post
    ...diseases, blindness, and diabetes. Directions and obstacles for future CRISPR therapies CRISPR technology ...systemic approaches, has its own unique set of obstacles. Thus far, several targeted therapies have taken...
  13. 5 Tips for Troubleshooting Viral Transductions

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    Blog Post
    ...that have helped me troubleshoot some technical obstacles when working with virus: 1. Viral vectors undergo...solution can be obtained. 4. Enhancing virus-cell contact A variety of reagents can increase transduction...
  14. 10 Basic tips for mammalian cell culture

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    Blog Post
    ...PBS (Phosphate Buffered Saline), that comes in contact with the cells should already be at 37 °C. Most.... If they reach confluence, they stop growing (contact inhibition) and it takes them time to recover after...
Showing: 101 - 120 of 361 results