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We narrowed to 362 results for: tac

Showing: 101 - 120 of 362 results

  3. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...from a different DNA strand comes along, it can attack this covalent bond thus joining the two DNA strands...topoisomerase I from rebinding, which results in more intact molecules. (Note that the amount of salt you add...

  4. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...again write for fun eventually saw an English minor tacked onto my BSc. With this decision I had unwittingly... ours reflect a growing effort to address this obstacle in its many forms. A short stroll from my office...

  6. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...with a valid email address).  One may also simply attach important references that serve to further characterize...much appreciate feedback, so try out the site and contact us if you have any ideas or requests.  Or, if you...

  8. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...with a fluorescent protein remain functional. Attaching the fluorescent protein fusions allowed us to ...the bacteria apart; however, they still remain attached to the ciliate. It’s thought that they function...

  9. Antibodies 101: ChIP

    Type
    Blog Post
    ...separate them. Most proteins are not likely to remain attached to the DNA throughout the sample processing and...sample may need more or less cross-linking to firmly attach the proteins to the DNA, depending on how tightly...

  11. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ... protein. These RNA molecules are vulnerable to attack by both cytoplasmic and nuclear nucleases, as they...group of the ribose to protect it from nucleolytic attack. Another stabilizing sugar modification, found ...

  12. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...method relies on multiple secondary antibodies attaching to a single primary antibody, it is sometimes ...reality, multiple primary antibodies can and do attach to a single target protein, so this assumption ...

  13. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ... used to turn “on” and “off” MEK1 activity by attaching two dimerizing photoswitchable Dronpa (dron= a...at sites homologous to the locations they were attached to in MEK. The photoswitchable versions of MEK1...

  14. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ... look at their profile. Make your professional contact information available. When I am speaking at a ...touch if you have business or follow up. Use the "Contact info" link right below their photo. 2) Personalize...

  15. RNA Extraction Without A Kit

    Type
    Blog Post
    ... lysis, RNA is degraded. To effectively isolate intact RNA, a fast, strong protein denaturant would be...ultracentrifuged for up to 20 hours to separate the intact RNA from DNA and proteins. Although very effective...

  17. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...another optimized platform. Gene Stacking As the name implies, gene stacking is the use of a combination of...

  18. CRISPR in the Clinic

    Type
    Blog Post
    ...diseases, blindness, and diabetes. Directions and obstacles for future CRISPR therapies CRISPR technology ...systemic approaches, has its own unique set of obstacles. Thus far, several targeted therapies have taken...

  20. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ... for a knock-out in your experiments? AAV-flex-taCasp3-TEVp from Nirao Shah's and Jim Well's lab induces...use viral prep in serotype AAV5. Find AAV-flex-taCasp3-TEVp here The red-shifted channelrhodopsin (C1V1...
Showing: 101 - 120 of 362 results