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We narrowed to 167 results for: tox

Showing: 101 - 120 of 167 results

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Viral Vectors 101: Viral Applications

Type

Blog Post

...plasmid. Viral transduction is also generally less toxic to cells than transfections, preserving cell viability...
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

Type

Blog Post

...As nucleases may have off-target effects and/or toxicity, it would be advantageous to instead use a preexisting...
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

Type

Blog Post

...desired product while avoiding the accumulation of toxic intermediates or the shuttling of the intermediates...
RaPID Detection of RNA-protein Interactions

Type

Blog Post

...labeling approach, is an alternative option but it’s toxic to tissues and model organisms. Detecting RNA-protein...
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

Type

Blog Post

...results to rapamycin inhibition. To assess TRIM21 toxicity, Clift et al. created stable cell lines overexpressing...
Giving gRNAs a Facelift - Synthetic and Beyond

Type

Blog Post

...substitutions are slightly more efficient and less toxic to mammalian cells (Allen, et al., Frontiers in ...
Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

Type

Blog Post

...Slaymaker et al. showed that these mutants are not toxic to HEK cells and that similar mutations can improve...
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

Type

Blog Post

... using the rTetR system, but Cre-DOG lacks the toxicity that can be seen with high levels of transcription...
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

Type

Blog Post

...stop it from being used. One thing is that Cas9 is toxic. When cells carry CRISPR circuitry, it can slow ...
Antibodies 101: Stripping and Reprobing Western Blots

Type

Blog Post

...added fresh to solutions. It is also volatile and toxic (and smells bad!), so be sure to use it under a ...
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

Type

Blog Post

... the plasmid will produce anything dangerous or toxic to humans in bacteria, select “yes” in the drop-...
Adenoviral Vector Production and Troubleshooting

Type

Blog Post

... long-term transgene expression and negligible toxicity. However, their production is more complex and...
Rabies and Neuronal Tracing

Type

Blog Post

...First, these high levels of viral replication are toxic. Second, infecting subsequently higher and higher...
5 Tips for Troubleshooting Viral Transductions

Type

Blog Post

... efficiency in cells that are sensitive to the toxicity of polybrene (e.g., hematopoietic or primary cells...
Viral Vectors 101: Producing Your rAAV

Type

Blog Post

...performed 12–18 hours post-transfection to help remove toxic transfection reagents and keep the cells happy. ...
Fluorescent Proteins 101: Fluorescent Protein Timers

Type

Blog Post

...aggregation-prone tetrameric fluorescent proteins can be. Cytotoxicity, improper localization, and decreased functionality...
Plasmids 101: 5 factors to help you choose the right cloning method

Type

Blog Post

... for a single insert), mostly due to the use of toxic ccdB to prevent any non-recombinant vectors from...
10 Basic tips for mammalian cell culture

Type

Blog Post

...acid (a by-product of cellular metabolism that is toxic to the cells), and need to be split very soon. There...
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

Type

Blog Post

...genome, removing the risk of oncogenicity or genotoxicity seen with other vectors (Lukashev et al., 2016...
CRISPR 101: Mammalian Expression Systems and Delivery Methods

Type

Blog Post

...Infects dividing and non-dividing cells. AAV is least toxic method for in vivo viral delivery. Cas9 mRNA and...

Showing: 101 - 120 of 167 results

Results per page:

Viral Vectors 101: Viral Applications

Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

RaPID Detection of RNA-protein Interactions

Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

Giving gRNAs a Facelift - Synthetic and Beyond

Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

Antibodies 101: Stripping and Reprobing Western Blots

Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

Adenoviral Vector Production and Troubleshooting

Rabies and Neuronal Tracing

5 Tips for Troubleshooting Viral Transductions

Viral Vectors 101: Producing Your rAAV

Fluorescent Proteins 101: Fluorescent Protein Timers

Plasmids 101: 5 factors to help you choose the right cloning method

10 Basic tips for mammalian cell culture

Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

CRISPR 101: Mammalian Expression Systems and Delivery Methods

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