We narrowed to 172 results for: tox

1. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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   ...results to rapamycin inhibition. To assess TRIM21 toxicity, Clift et al. created stable cell lines overexpressing...

2. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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   ...desired product while avoiding the accumulation of toxic intermediates or the shuttling of the intermediates...

3. RaPID Detection of RNA-protein Interactions

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   ...labeling approach, is an alternative option but it’s toxic to tissues and model organisms. Detecting RNA-protein...

4. Giving gRNAs a Facelift - Synthetic and Beyond

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   ...substitutions are slightly more efficient and less toxic to mammalian cells (Allen, et al., Frontiers in ...

5. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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   ...Slaymaker et al. showed that these mutants are not toxic to HEK cells and that similar mutations can improve...

6. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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   ... using the rTetR system, but Cre-DOG lacks the toxicity that can be seen with high levels of transcription...

7. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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   ...stop it from being used. One thing is that Cas9 is toxic. When cells carry CRISPR circuitry, it can slow ...

8. Adenoviral Vector Production and Troubleshooting

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   ... long-term transgene expression and negligible toxicity. However, their production is more complex and...

9. Rabies and Neuronal Tracing

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   ...First, these high levels of viral replication are toxic. Second, infecting subsequently higher and higher...

10. Antibodies 101: Stripping and Reprobing Western Blots

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    ...added fresh to solutions. It is also volatile and toxic (and smells bad!), so be sure to use it under a ...

11. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    ... the plasmid will produce anything dangerous or toxic to humans in bacteria, select “yes” in the drop-...

12. 5 Tips for Troubleshooting Viral Transductions

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    ... efficiency in cells that are sensitive to the toxicity of polybrene (e.g., hematopoietic or primary cells...

13. Fluorescent Proteins 101: Fluorescent Protein Timers

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    ...aggregation-prone tetrameric fluorescent proteins can be. Cytotoxicity, improper localization, and decreased functionality...

14. Viral Vectors 101: Producing Your rAAV

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    ...performed 12–18 hours post-transfection to help remove toxic transfection reagents and keep the cells happy. ...

15. Plasmids 101: 5 factors to help you choose the right cloning method

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    ... for a single insert), mostly due to the use of toxic ccdB to prevent any non-recombinant vectors from...

16. 10 Basic tips for mammalian cell culture

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    ...acid (a by-product of cellular metabolism that is toxic to the cells), and need to be split very soon. There...

17. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    ...genome, removing the risk of oncogenicity or genotoxicity seen with other vectors (Lukashev et al., 2016...

18. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    ...Infects dividing and non-dividing cells. AAV is least toxic method for in vivo viral delivery. Cas9 mRNA and...

19. Light Sheet Fluorescence Microscopy

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    ...order to improve axial resolution and reduce phototoxicity for imaging live specimens, they combined light...

20. AAVs CREATed for Gene Delivery to the CNS and PNS

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    ...long-term expression; and 3) they have limited toxicity. However, a key challenge of using AAVs for neuroscience...