Pouring LB Agar Plates
Type
Protocol
...Reagents 37 g pre-mixed powder consisting of: 5.0 g yeast extract 10.0 g peptone from casein 10.0 g sodium...
We narrowed to 138 results for: yeast
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To Codon Optimize or Not: That is the Question
TypeBlog Post...organisms for expression in prokaryotic systems or yeast (Lanza et al., 2014). For example, it may be worth... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...open-reading frame (ORF) libraries have been generated for yeast (Kim et al., 2019) and fruit flies (Bischof et al... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog Post...available for many expression systems: bacterial, yeast, and mammalian. These plasmids contain both untagged... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog Post...antibiotic — it is usable in eukaryotic cell culture, yeast, bacteria, and even plants! The zeocin resistance... -
Plasmids 101: Codon usage bias
TypeBlog Post...specific secondary structures in 10 closely related yeast strains. Adaptation to changing conditions - Organisms... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post.... These are less well known fungi (they are not yeast or mushrooms) but there are over 1,000 species. ... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog Post... types including (but not limited to) bacteria, yeast, plants, drosophila, worms and mammals (Browse plasmids... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog Post...grow cells in Lennox LB (10 g/L tryptone, 5 g/L yeast extract, and 5 g/L NaCl) or on LB-agar plates containing... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post...ability to invert or ‘flip DNA’, is derived from the yeast S. cerevisiae (Gronostajski and Sadowski 1985). ... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post... unlike infections with larger microbes such as yeast, fungi, or bacteria, mycoplasma can be extremely... -
CRISPR References and Information
TypeCollection... support for bacteria ( E. coli, B. subtilis ), yeast ( S. cerevisiae ), worm ( C. elegans ), fruit fly...genomes are human (hg19 & hg38), mouse (mm10), and yeast (strain w303). Developed by Venetia Pliatsika and... -
Modular Cloning Guide
TypeGuide...biosensors in yeast (requires MoClo-YTK parts for use). Yeast GoldenBraid Cloning System and Toolkit Yeast Expression...for S. cerevisiae expression. Multiplex Yeast Toolkit (MYT) Yeast Expression, CRISPR Tom Ellis 96 plasmids...Aspergillus species. MoClo Yeast Secretion and Display (YSD) Toolkit Yeast Expression Paul Young 34 plasmids...surface display of proteins of interest. Yeast Secrete and Detect Yeast Expression Sylvestre Marillonnet Plasmids...secretion in yeasts S. cerevisiae and P. pastoris (a.k.a. K. phaffii ). POMBOX MoClo YTK Yeast Expression...transcription units in the oleaginous yeast Y. lipolytica . EXPRESSYALI Yeast Expression Irina Borodina Plasmids...into Y. lipolytica chromosomal loci. Yeast GPCR-sensor Toolkit Yeast Expression Tom Ellis Plasmids for constructing... -
Fluorescent Protein Guide: Biosensors
TypeCollection...sensor for Yeast (AT1.03 derived) Dynamic analysis of cytosolic glucose and ATP levels in yeast using optical...osmolarity biosensor, for expression in E. coli , yeast, or plants Intrinsically disordered protein biosensor...pH-biosensor for reporting vacuolar turnover in yeast (cytosolic and mitochondrial) Rosella: a fluorescent... vacuolar turnover of cytosol and organelles in yeast. Autophagy. 2008 Feb;4(2):205-13. Mark Prescott ...activated by G-protein-coupled receptors (GPCRs) Yeast GPCR-sensor Toolkit includes plasmids for constructing... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog Post...characterisation of fluorescent proteins in budding yeast." Scientific reports 9.1 (2019): 2234. PubMed PMID... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog Post...While it’s possible to produce scFvs in mammalian, yeast, plant, and insect cells, they are most often expressed... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog Post...promoters to facilitate gene expression in bacteria, yeast, mammalian cells, insects, and even Xenopus oocytes... -
Plasmids 101: Biotinylation
TypeBlog Post...biotinylate AviTag expressing proteins in bacteria, yeast, insect and mammalian cells. Check out Kay et al... -
Antibody Plasmid Collection
TypeCollection... expressing these in bacteria, mammalian cells, yeast, plants, or insect cells. Benefits include consistency... -
Molecular Cloning Techniques
TypeGuide...Created with BioRender.com. Yeast-mediated Cloning and Oligonucleotide Stitching Yeast-mediated cloning is very...powerful recombination abilities of yeast. By simply transforming into yeast two (or more) fragments of dsDNA... cost-saver for labs working with yeast. Figure 7: Summary of yeast-mediated plasmid cloning and oligonucleotide...Golden Gate & MoClo Ligation Independent Cloning Yeast-Mediated & Oligo Stitching Resources Molecular cloning...manner. To accomplish this, you can transform into yeast the fragments of DNA to be fused along with custom...