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We narrowed to 641 results for: ato

Showing: 601 - 610 of 641 results
  1. Centrifugation

    Type
    Protocol
    ... the two, use a conversion calculator or chart like the G Force calculator (Link opens in a new window...Introduction A centrifuge is a common piece of laboratory equipment used to separate different components...
  2. Immunocytochemistry

    Type
    Protocol
    ...water Microscope slide Anti-fade mounting medium Laboratory wipes 15 mL conical tubes 50 mL conical tubes...paraformaldehyde and follow your institution's laboratory safety guidelines for disposing of waste in the...remove the coverslip. Blot the coverslip with a laboratory wipe to remove excess liquid. Add 1 drop of anti-fade...
  3. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...pipette 200–1000 µL single channel pipette CO 2 incubator Pipet controller Hazardous waste container Microscope...orange/yellow if using a standard phenol red pH indicator) because the buildup of waste products could be...difficult to find. Leave the cells undisturbed in an incubator for 7–14 days. After ~7 days, scan the plate for...
  4. CRISPR Library Amplification

    Type
    Protocol
    ...require modifications dictated by the originating laboratory for optimal results. If you obtained the pooled...limited. Equipment Table top centrifuge BioRad Electroporator (MicroPulser TM , Bio-Rad 1652100) Reagents...time for a total of eight electroporations): Electroporator Conditions: Bio-Rad Micropulser Ec1 0.1 cm ...
  5. Protocol - How to Streak a Plate

    Type
    Protocol
    ...loop Bunsen burner (or other small flame source) Incubator Marker Reagents LB agar plate (with appropriate...resistance and your initials. Labeling within a laboratory setting is important for organization, and it...
  6. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...spreading of antibiotic makes it easy for an investigator to conveniently plate and select transformed...spreading Bunsen burner (or other small flame source) Incubator Reagents 6 cm diameter LB/agar plates without ...
  7. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...Addgene’s Mammalian RNAi Tools page. Several other laboratories have deposited pLKO derived vectors that may... temperature. g. Retrieve HEK-293T cells from incubator. The cells should be 50-80% confluent and in DMEM...should be decontaminated with at least 10% bleach. Laboratory materials that come in contact with viral particles...
  8. Water Bath Protocol

    Type
    Protocol
    ... lab! Introduction A water bath is a piece of laboratory equipment that helps bring your materials to .... Water baths can also be placed in walk-in refrigerators to achieve a temperature between 4°C and room...
  9. Video Library

    Type
    Protocol
    ... Protocols Video protocol guides for standard laboratory procedures. (Link opens in a new window) Video...Electrophoresis Gel electrophoresis is the standard laboratory procedure for separating DNA by size for visualization...
Showing: 601 - 610 of 641 results