We narrowed to 653 results for: EPO
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TypeGuide...and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a, it will release... using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the reaction...following the amplification protocol specified by the depositing lab. After amplification, you’ll need to use ... target sequence, it promiscuously cleaves the reporter as well, and a quantifiable signal is produced...CRISPR 101 eBook (3rd edition) CRISPR software and depositor protocols JOVE video reprint: Protocol for genomic...NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC Additional Cas9s from...all-in-one CRISPR/Cas9 vector system. Scientific Reports , 4 (1). PMID: 24954249 Shechner, D. M., Hacisuleyman...
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TALENs for Endogenous Zebrafish Genes
TypeCollection...TALENs for Endogenous Zebrafish Gene Targets Recent reports have shown that engineered TALENs can be used to... -
Optogenetics AAV Preps
TypeCollection...quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV collection, including... -
Deisseroth INTRSECT Collection
TypeCollection... Depositor Collections INTRSECT Plasmid Collection INTRSECT... -
Plan Your Experiment
TypeGuide...promoter is typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive...vector or separate transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify...Additional Resources: CRISPR protocols from Addgene depositors JOVE Video Reprint: Protocol for genomic deletions...in question (see CRISPR protocols from Addgene depositors ). Deliver Cas9 and gRNA Choose a delivery method...facilitates CRISPR/Cas9-based genome editing. Scientific Reports . 5 , 11315. PMID: 26066060 Zuris, J. A., Thompson... -
The Pleiades Promoter Project
TypeCollection...MiniPromoters MiniPromoter Source Gene Construct Reporter Negative Control N/A pEMS1312 cre N/A pEMS1301... -
Genomic Deletions in Mammalian Cell Lines
TypeCollection...transfection conditions for each cell line with a reporter construct to ensure robust plasmid delivery before... -
Tetracycline Inducible Expression
TypeCollection...efficacy by fluorescence, use Plasmid #35626 as a reporter for cDNA target. rtTA3 Ptet, miniCMV Howard Gu... -
Allen Institute for Brain Science AAV Enhancer Collection
TypeCollection... Depositor Collections Allen Institute for Brain Science ... -
Optogenetics Guide
TypeGuide...optical switches Sensors are genetically-encoded reporters of molecular signals; e.g., calcium indicators...optogenetics tool - they typically allow the fast depolarization of neurons upon exposure to light through direct... trafficks well to the membrane. Excitatory (Depolarizing) ChR Variants. The optogenetic toolbox has been...light-gated, cation channels resulting in excitation (depolarization) of the neuron. Feature enhancements include...