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We narrowed to 641 results for: ato

Showing: 631 - 640 of 641 results
  1. Western Blot

    Type
    Protocol
    ...the Top Stack to one side and discard the white separator. Keep the Bottom Stack in the plastic tray. Place...
  2. Chemogenetics Guide

    Type
    Guide
    ... Experiment References Chemogenetic tools are actuators for specific cellular pathways, receptors, or ...of ion channels arePharmacologically Selective Actuator Modules (PSAMs, pronounced SAMs). PSAMs are engineered...Depending on your answer, you'd pick a chemogenetic activator or inhibitor, respectively. DREADDs or PSAMs or...delivery. Chemogenetic Ligand The chemogenetic actuator chosen and the context of the experiment will ...such as DCZ versus CNO. Delivery Chemogenetic actuators are delivered in vivo through viral injection ...Selective Modulation of cAMP Production in Striatopallidal Neurons. Neuropsychopharmacol, 38 (5), 854–...Deschloroclozapine, a potent and selective chemogenetic actuator enables rapid neuronal and behavioral modulations...
  3. AAV ddPCR Titration

    Type
    Protocol
    ... Transfer the cartridge holder to the droplet generator. Close the lid and wait for the droplets to be...
  4. Lentivirus ddPCR Titration

    Type
    Protocol
    ... Transfer the cartridge holder to the droplet generator. Close the lid and wait for the droplets to be...
  5. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...gamma-retroviral vectors that can be safely used in laboratory settings. These engineered gamma-retroviral vectors...increased safety when producing viral vectors in a laboratory setting. Figure 1: Wild-type gamma-retrovirus... Design. In Retroviruses . Cold Spring Harbor Laboratory Press. NIH Bookshelf De Ravin, S. S., Su, L.,...Woodchuck hepatitis virus post‐transcriptional regulatory element; stimulates the expression of transgenes...
  6. Plan Your Experiment

    Type
    Guide
    ...fusion with transcriptional activator (such as dCas9-VP64) Many different activator systems exist, including...requires dCas9 to be fused to transcriptional activators that need to be within a given range of the transcription...
  7. Adenovirus Guide

    Type
    Guide
    ...diseases in humans and other animals, most commonly respiratory infections, such as the common cold. The immune... express them alongside a guide RNA and other regulatory elements. The packaging capacity of rAdV vectors...such as expressing cytokines and other immune-modulatory molecules. Another strategy has been to use oncolytic...
  8. Molecular Biology Reference

    Type
    Guide
    ...can be replicated easily and efficiently in a laboratory setting. Are stable — Plasmids are stable long-term... contain a promoter sequence, a transcription terminator sequence, and the inserted gene. An expression... and the sequence outputted in the form of a chromatogram. Sanger can sequence approximately 500–1,000...
  9. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ... factories for the new viral particles in the laboratory. The cell line used for rAAV vector production...packaging capacity, especially if any additional regulatory elements are needed. One strategy to overcome...Woodchuck hepatitis virus post‐transcriptional regulatory element; stimulates the expression of transgenes...
Showing: 631 - 640 of 641 results