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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 160 results for: ALC

Showing: 121 - 140 of 160 results

  12. Chemogenetics Guide

    Type
    Guide
    ... phospholipase C, which releases intracellular calcium stores. There are currently three Gq DREADDs based...

  15. Deep Dive: qPCR

    Type
    Blog Post
    ... Standard Curve?  Amplification Efficacy Calculation Amplification Efficacy Assumptions Standard...

  16. Cre-lox system

    Type
    Collection
    ...pLV-EF1-Cre-PGK-Puro Cre EF-1 alpha Lentiviral Alcudia 108639 fli1aCreERT2 Cre-ERT2; endothelial reporter...

  18. Lentivirus ddPCR Titration

    Type
    Protocol
    ... forget to factor this into your calculations later (see calculation example below). Seed 300,000 cells... a CSV file which will be used to calculate the titer. Calculations In the experimental setup above, the...20 200 40 400 80 800 160 1600 To calculate the titers, first calculate the number of viruses per cellular...glutaGRO, Corning 25-015-CI) 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect...over copies\ RPP30}$$ Use the viruses/genome to calculate the infectious titer: $$T = {V*C*D\over v}$$ Where...titer obtained from the appropriate dilutions to calculate the final infectious titer. Tips and Troubleshooting...green). To increase the accuracy of the titer, calculate an average of several dilutions. Figure 1: ddPCR...

  19. CRISPR Library Amplification

    Type
    Protocol
    ...new vented falcon tube for every two transformations. Each of the four 14 mL Vented Falcon Tubes should... mL of LB Agar per bioassay plate. 14 mL Vented Falcon Tubes (BD Biosciences, 352059) 3X LB Agar + Antibiotic...supports normal growth is expected to work. 50 mL Falcon Conical tubes (Fisher, 14-432-22) Reagent Preparation...Aliquot 3 mL SOC into each of four 14 mL Vented Falcon Tubes and have 1 mL SOC per electroporation readily...all liquid from cuvette and add to 14 mL vented Falcon Tube containing 3 mL SOC. Repeat for each of the...DNA-Endura from cuvette. Shake four 14 mL Vented Falcon Tubes at 30-37 ℃, 225 rpm for 1 hour. After the...

  20. Fluorescence Titering Assay

    Type
    Protocol
    ... of PBS. Calculate the fraction of fluorescent-positive cells in each well. When calculating titer, only...mL), Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect...remaining well, a cell count is required for calculating the titer. Incubate for 48–72 h. Gently aspirate...leading to underestimation of the true titer. Calculate the transduction units per mL (TU/mL) using either...
Showing: 121 - 140 of 160 results