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We narrowed to 545 results for: SEC

Showing: 121 - 140 of 545 results

  1. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...kb. ITRs are inherently unstable due to their secondary structure, palindromic nature, and high GC content...typically fails to sequence through ITRs due to their secondary structure and high GC content, but some companies...sites, and then excise the sequence between the second pair. The final result is a permanently flipped...

  2. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...considerable number of researchers, I had high hopes of securing tenure in a British or Irish University where ... courses taken during my PhD and Undergraduate sections and brought in more focus on the list of conferences...towards me as a product and not me as a scientist. Second, I updated my LinkedIn profile, matched it with...

  3. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...Xin has been able to develop photoswitchable Intersectin, Cdc42, Raf1, Mek1, Mek2, Cdk5, and Cas9. Xin...consuming in the first case and nonspecific in the second. Fatima therefore set out to design and produce...the amount of HMO originally in the sample. The second sensor is a fusion protein consisting of sfGFP ...

  4. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...protein secretion, making harvesting recombinant proteins a cinch. This is because it doesn’t secrete its ...proteins, making the recombinant protein its major secretion. Beyond sheer protein production, K. phaffii is...

  5. Hot Plasmids - January 2023

    Type
    Blog Post
    ...something smokin'? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) here. Here's...option for targeting GFP. But why might you need a second GFP antibody?  Similarities:  Generated against...mEGFP-HRas (from Plasmid 18662). See the Applications section on the Anti-GFP [N86/8R] page for more details....

  6. 5 Great Apps for Lab Life

    Type
    Blog Post
    .... This app also has a great educational section. This section holds the Gibco Cell culture manual and ...favorite lab app, please share it in the comments section below! Many thanks to our guest blogger Sean Stacey...

  7. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ... the substrate coelenterazine and is likely the second most commonly used luciferase. Other organisms ...Peak emission (nm) Size (kDa) Substrate Cofactors Secreted Luminous shrimp(Oplophorus gracilirostris) 455...cell membrane and deliver the substrate; however, secreted luciferase or the use of alternative substrate...

  8. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ...overexpression and knockdown. Addgene also has a number of insect vectors to consider. An easy way to see what is...available is to browse Addgene’s Collection under the insect expression system or by filtering by fly as species...organs of interest, such as the intestines, can be dissected at various times throughout the lifespan of the...

  9. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ... plasmid page under the "Resource Information" section. Analyze your plasmid sequence For more immediate...also click one location in the plasmid and then a second location to select the sequence between the two...what you think about the updates in the comments section below and keep checking in at blog.addgene.org ...

  10. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ...bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular Cytology and Van Leeuwenhoek Centre...Joachim Goedhart is an assistant professor at the Section of Molecular Cytology and Van Leeuwenhoek Centre...joachimgoedhart.   Marieke Mastop is a PhD candidate at the Section of Molecular Cytology (University of Amsterdam)...

  11. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...   Once you get your exciting new antibody, the second thing you’ll want to do is store it away safely...kit, and is readily available on any number of secondary antibodies. And look - if it ain’t broke, don’...fluorophore strength with protein abundance.    Second, you’ll need to consider spectral overlap. Fluorophores...

  12. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ...business competitions. When the team was unable to secure enough money to sustain a startup, he went to work... the data must be reproducible by someone else. Second, "an entrepreneur must be OK with the constant ...or product? Please let us know in the comments section! And read on for more great information about starting...

  14. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ...PHP.eB compared to the existing ChR2(H134R).  In the second experiment, ChRger2 was delivered and activated...-invasive methods to the neurons in the right secondary motor cortex (M2). Since M2 is located on the ...lab could thin the mice’s skull near the M2 and secure a fiber optic cannula to the skull, leaving the...

  15. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ...bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular Cytology and Van Leeuwenhoek Centre...Joachim Goedhart is an assistant professor at the Section of Molecular Cytology and Van Leeuwenhoek Centre...joachimgoedhart.   Marieke Mastop is a PhD candidate at the Section of Molecular Cytology (University of Amsterdam)...

  16. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...present. One common example is using pre-adsorbed secondary antibodies (which recognize other antibodies) ...this can increase background staining (see next section), it may be advantageous if you are trying to detect...useful for certain research applications. Many secondary antibodies are polyclonal, as their function is...

  17. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ..., my mind went completely blank. After several seconds of truly panicked silence, I opened my mouth to... rousing success–the response was a full sixty seconds of silence–but it was thorough and coherent.  To...nerve to do what I did. Until that panicked, split-second decision, I had always thought I had an obligation...

  18. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...bookshelf. Pro tip The “cited by” and references sections of a PubMed entry are great places to look for... that cite the background, methods, or results section is also displayed. Saving papers Papers can be...that contain these terms. You can also create intersects in your feed which means only papers than contain...

  19. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...which does not have primary and only contains secondary antibodies, allowing us to only image autofluorescence...independent screening”, where they look at the same section and determine the antibody’s reliability through...neuroanatomy without the fear of referencing a wrong section. The antibody screening and development of a focused...

  20. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...antibody, a primary-secondary pair is used. This amplifies the signal as multiple secondary antibodies can ...can bind to a single primary antibody. The secondary antibody is conjugated to an enzyme for visualization...
Showing: 121 - 140 of 545 results