We narrowed to 778 results for: Dos

...continue reading for more details. How much time do you spend reading scientific literature? Very often...’t save you the hard work of reading papers and doing experiments but we can definitely give you credit...envisions a world where the impact of teaching and doing science is rapid, concrete and open to innovation...

...successfully takes up plasmid DNA. Among those that do, some now have plasmids that contain your gene of...you’re seeking. Fortunately, there are many ways to do this involving positive selection, negative selection...cells that contain a specific gene survive. The rest do not grow. While there are many ways to design your...

...stability Figure 1: So many choices! What to do? The main factors that can affect DNA stability...stability of your DNA, it will limit what you can do with these samples. This is because divalent cations...than water in these conditions.  The next time you do a plasmid prep, plan in detail before you drop that...

...researchers are communicating across disciplines or doing different tasks. But these challenges can be exaggerated... with each other in person. One possibility is to do a lab exchange, where researchers spent time in each...cohort, neither of which I would have been able to do from my home lab. Scientific conferences and workshops...

... more information on FLEx switch and what you can do with it, read Addgene's blog on FLEx Vectors . Flp-IN... Resources Content last reviewed on 31 July 2025. Do you have suggestions for other plasmids that should...

...Optogenetics Content last reviewed: 25 September 2025 Do you have suggestions for other plasmids that should...

...compelling, and I encourage you to hold one. Why do we need physical models of protein structure? Structural... you can only wonder what I am talking about. How do researchers use physical models of protein structure...and dangerous. Subscribe to CRISPR Blog Posts How do educators and students use molecular models?   We...human body?” … and probably most importantly… "how do we know this?” Over the past fifteen years, the CBM...

...Entrepreneur, Motivational Speaker Do you have a difficult supervisor, or do you have to work with difficult...problem and leave your emotions out of the discussion Do not assume that your supervisor is familiar with ...ideas, but he or she probably has reasons for it. Do not take criticism personally or get defensive. Look...

...primary antibody controls in your experiments. If you do find high levels of background staining by the secondary...binding Direct immunohistochemistry (Figure 2A)  How do you avoid having your secondary antibody bind endogenous...common solutions to avoid background staining when doing species on species IHC. (A) One solution is to use...

...2016; Torres & Casadevall, 2008) but…does it really do anything? Fc functions The answer is, yes, it does...Fc effector functions and all that antibodies can do! For instance, some Fc receptors, once bound to an...structure and isotype determination, remember that it can do so much more than that!    References and resources...

...Enhancer traps do not need to be integrated within an intron or exon of a gene, and thus they do not disrupt...they are directly integrated into it. Enhancer traps do not need to be inside of a gene’s open reading frame...

...’t truly address the question of what I wanted to do when I grew up until I was all but finished my undergraduate...curiosity did not ultimately translate to a desire to do the research itself. Several years of exclusively...audience and how to utilize a wide array of media in doing so—can prove invaluable in the pursuit of public...

...transition state or specific configuration. So how do you ensure that you have the right antibody for the...configuration in your assay. Did you have a denaturing step? Do you want to capture the protein bound to anything...elucidate information about what a given protein is doing or changing in different conditions. Just remember...

...“Synthetic biology, in particular, the ability to do DNA synthesis, is evolving so fast that it is a really...technological change happens really fast,” Thompson says. To do this, Thompson reached out to MIT labs in the Synthetic... engineered? Where did it come from? What does it do? Those are the sets of questions one would want to...

...alone; a researcher doing some experiments in Brazil can have the same issues as one doing experiments in ...sometimes wish they could live differently than we do day to day. We discovered that it can be good for...

...with a PhD in biology. Depending on what you want to do, that’s not necessarily bad news, but it is an argument... science with the community. Some of them you can do today! 10 ways to share your science 1)   If you ...talk – really talk – about the research you are doing in the lab with your mother or father or your best...

...support your career development as your mentor. How do you make the most of this new relationship? Consider...skills in which you have expertise or knowledge. Where do these intersect? Work with resources: Choose books...take larger goals (e.g. Figure out what I want to do after I get my PhD) and break them down into smaller...

...questions! How much virus should I inject? How long do I need to wait? What serotype and promoter should...the answers, but we’re betting that your colleagues do. That’s why we’re launching the AAV Data Hub. The...

...the population that have an edit. I didn’t do this, but to do it you would need a pretty good gel image...Cas9-expression cell line. Especially if you’re doing a screen. I made a few monoclonal lines (Figure ...expansions (monoclonal, 1-5). Wild-type A549 cells do not express CAs9 and are included as a control (WT...couldn’t get a good image. You could probably also do Western blotting against GFP expression, but I didn...designing primers flanking my edits in genomic DNA and doing PCR on genomic DNA. The surveyor assay itself was...

...How do you know if you actually have DNA in your tube without seeing it? There are many ways to do this... time to set up in the lab, you might not have to do the calculation yourself as many fluorometers will...

... our FAQ on What is virus associated DNA, and why do I have to order it? Don’t See What You’re Looking...

...determines that our current queue is too high and we do not expect to meet the general turnaround time, Addgene...

...AdEasy™ System Content last reviewed: 12 November 2025 Do you have suggestions for other plasmids that should...

...Satellite colonies are small colonies of bacteria which do not contain the plasmid and its associated resistance...effectively selecting plasmid-containing bacteria. So… why do some people still use amp? Carb is expensive (relative...Tn5 element, but thankfully, most competent cells do not express this factor. Zeocin is handy because ...above antibiotics would work for you, they likely do! Give our summary table a look for a quick recap ...

...explaining how your experience and skills would help you do the job as described. Let’s imagine you’re applying...example, if you don’t have experience with ddPCR, but do have experience with qPCR, you could talk about how...that are excited about their work and mission.  How do I know what to write about? If you’re struggling ...that skill. If the answer is yes, ask yourself “how do I know that I have that skill?” The answer to that...

...are the perfect person to be writing the review! Doing so is a great way to develop your ability to write...here, I will share my process with you. But before I do that, let me say that I was by no means an expert...overthink it. And to quote my adviser, "you just gotta do it." At the end of the day, someone has to write ...article and that someone is going to be you. So, just do it! I will also paraphrase what I have heard many...

...people develop the disease while others never do, but we do know that there are some genetic factors involved...much of a danger of getting the disease, even if you do happen to be exposed to asbestos. However, as the...cancer is diagnosed so late that these treatments do not work very well. The best option in such instances... mesothelioma many years later. While there is a dose-response relationship (the higher the exposure, ...

... why you might enjoy doing it. I’ll also talk about the skills you’ll need to do well as a healthcare ...healthcare consultant and what you could do to break into the field. What is healthcare consulting? Healthcare...pulling your weight with your scientific expertise. Do I have to stick with consulting in the long-term?...and services that help diabetic patients manage doses and timings of their drug treatments. The process...

...the situation in the future. Addgene: What advice do you give to post-docs and graduate students as they...publications in a short period of time and may need to do some fashionable projects. However, I hope that they... they also have long-term goals and continue to do research to realize their vision. Their progress might...they'll be completely different from what we are now doing in my lab then when they become independent. Looking...

...same transcript. Of course multicistronic vectors do not have to exclusively be used as a means of detection...levels between two or more genes is important. How do multicistronic vectors work? Scientists have "borrowed...commonly found in bicistronic vectors; however, they do have some disadvantages. These elements are quite...of the peptide to improve cleavage efficiency. How do I get started? If you are looking to co-express your...