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We narrowed to 731 results for: SON

Showing: 181 - 210 of 731 results

  1. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...genetic tools. Mobile-CRISPRi from the labs of Jason Peters, Oren Rosenberg, and Carol Gross provides...Mobile-CRISPRi is introduced using the bacterial Tn7 transposon system. This system has two parts: a plasmid ...Introducing Mobile-CRISPRi into bacteria. Top: A Tn7 transposon containing the CRISPRi components and a plasmid...methods 2.6 (2005): 443. PubMed PMID: 15908923. Johnson, Christopher M., and Alan D. Grossman. "Integrative...4.2 (2019): 212. PubMed PMID: 30675034. Peters, Jason M., et al. "Enabling genetic analysis of diverse...

  2. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...As a person with many roles and responsibilities, I have to think a lot about how to balance those roles...your community’s schools, here are a few tips and lessons I’ve picked up from my recent experiences. STEM...history helped demonstrate that a) I was a real person who used to be a middle schooler himself and b)...back and trying again next year. There are many reasons why you might want to reach out to your school ...tumultuous time in scientific history. Whatever your reasons, let us know how it goes! Acknowledgements: I...

  3. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...Kostick, K., Torgerson, L., Muñoz, K. A., Hsu, R., Kalwani, L., Sierra-Mercado, D., Robinson, J. O., Outram... rat CNS: AAV PHP.EB vector dose–response and comparison to AAV PHP.B. Gene Therapy, 25(5), 392–400. https...00981-0 Wright, J. F., Le, T., Prado, J., Bahr-Davidson, J., Smith, P. H., Zhen, Z., Sommer, J. M., Pierce... Jason Nasse...

  4. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ... to put to rest a common misconception. I find personal bias often unintentionally plays a big role in...varied in how they prefer to consume information. Personally, I would much rather read the instructions than... accessible via web searches; likewise, most in-person events and venues will have a solid understanding...Primarily useful for data-focused stories. In-person lectures, presentations, etc… Low (except for ...  Rachel Leeson...

  5. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ... Jake Watson and Javier García-Nafría from the MRC Laboratory of Molecular Biology. Jake Watson is a postdoctoral...This post was contributed by Jake Watson and Javier García-Nafría from the MRC Laboratory of Molecular...receptor function.     Follow them on Twitter @jakefwatson and @JGarciaNafria.  References 1. Jones, Douglas...PMCID: PMC331480. 3. García-Nafría, Javier, Jake F. Watson, and Ingo H. Greger. "IVA cloning: a single-tube...

  6. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...libraries that do just that (Doench et al., 2016, Sanson et al., 2018). These libraries utilize optimized...additional transcription factors to the targeted region (Sanson et al., 2018) . This tracrRNA was combined with...selected per gene and divided into two sets, A and B. Sanson et al. demonstrated that the Calabrese library ... more hits than the SAM library approach in a comparison study screening for vemurafenib resistance in...: 25494202. PubMed Centra PMCID: MC4420636.  5. Sanson, Kendall R., et al. "Up, down, and out: next generation...

  7. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...favorably to other methods. In a head-to-head comparison with Cas9, Cas9 modified 9/12 known off-targets... C., Rees, H. A., Packer, M. S., Badran, A. H., Bryson, D. I., & Liu, D. R. (2017). Programmable base ...doi.org/10.1038/nbt.3803 Koblan, L. W., Doman, J. L., Wilson, C., Levy, J. M., Tay, T., Newby, G. A., Maianti... Bohnuud, T., Fernandez, T. P., Gehrke, J. M., Grayson, P., Lee, K. D., Ortega, M. A., Sawyer, C., Schwaegerle..., Lapinaite, A., Newby, G. A., Thuronyi, B. W., Wilson, C., Koblan, L. W., Zeng, J., Bauer, D. E., Doudna...Levy, J. M., Yeh, W.-H., Zheng, C., Newby, G. A., Wilson, C., Bhaumik, M., Shubina-Oleinik, O., Holt, J....

  8. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    .... 4. Shaner, Nathan C., George H. Patterson, and Michael W. Davidson. "Advances in fluorescent protein... structure resembles images of the deity (see comparison image above). The church of the Flying Spaghetti...schools and was started in 2005 in Kansas by Bobby Henderson, a physics graduate from Oregon State University...New York. pp 719. 3.Rizzo, Mark A., Michael W. Davidson, and David W. Piston. "Fluorescent protein tracking...

  9. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...know your collaborators from other institutions personally on conference calls and make sure to maintain...collaborating with people outside my lab’s department, I personally felt that I had to overcome some hesitation to...the hard part: maintaining these relationships. Personally, I find this to be the most challenging and time-intensive...maintain a relationship - many of them involve personal meetings and common interest so naturally this...sharing. LinkedIn or ResearchGate allow you to send personal updates as messages and keep you up-to-date on...

  10. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...are addressed with ddPCR technology (Hindson et al., 2011, Hindson et al., 2013), which has been shown ...2011): 8604-8610.4. Hindson CM, Chevillet JR, Briggs HA, Gallichotte EN, Ruf IK, Hindson BJ, Vessella RL,... therapy 6.7 (1999). PubMed PMID: 10455443. 3. Hindson, Benjamin J., et al. "High-throughput droplet digital...

  11. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...service in 2016. We realized we could apply the lessons learned from our plasmid lab to develop a reliable...available. This prioritization happens for good reasons. We began offering our viral vector service because...our Viral Vectors 101 eBook and blog posts. The lessons learned from this process have shaped and supported...fnana.2019.00093 McDade, J. R., Waxmonsky, N. C., Swanson, L. E., & Fan, M. (2016). Practical Considerations... Rachel Leeson...

  12. How to Be an Excellent Trainee

    Type
    Blog Post
    ...advice, open doors for you, and teach you valuable lessons along the way. We think it’s so important that ... technician, or a graduate student. This is the person you report or talk to on a daily basis who will...scared to ask for some! You can email or ask in-person “Are there any papers you think would be useful...these things will help you get more out of the in-person training component. Documenting during training...take on trainees one day when you are the senior personnel! Happy training! Resources Resources on the Addgene...

  13. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...CAC trinucleotides during neuronal maturation (Tillotson & Bird, 2019) and is a global transcriptional ...rescue the neurological defects of Rett syndrome (Tillotson et al., 2017). Advancing gene therapies for Rett...Supplement/S51.002.abstract Sinnamon JR, Kim SY, Fisk JR, Song Z, Nakai H, Jeng S, McWeeney SK, Mandel G (2020)... . https://doi.org/10.1016/j.omtm.2017.04.006 Tillotson R, Selfridge J, Koerner MV, Gadalla KKE, Guy J...398–401 . https://doi.org/10.1038/nature24058 Tillotson R, Bird A (2020) The Molecular Basis of MeCP2 ...

  14. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    ...comes to genome editing, CRISPR is a name that resonates with nearly every biologist, academic, and researcher...also need a short sequence motif called a TAM (transposon-associated motif), which is positioned like Cas12a...parts: a structural scaffold derived from the transposon's right-end element, and a 20-nucleotide (nt) ...genome editing in monocot and dicot plants using a Transposon-Associated TNPB system. In Springer protocols...). A miniature alternative to Cas9 and Cas12: Transposon‐associated TnpB mediates targeted genome editing...

  15. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...organisms have different codon usage biases? The reasons for varied codon preferences among organisms aren...aren’t completely understood, but some possible reasons include: Metabolic pressures - it takes cellular...recognize codons found in highly expressed genes (Emilsson and Kurland, 1990). Controlling gene expression...15678157. PubMed Central PMCID: PMC1299251. 3. Emilsson, Valur, and Charles G. Kurland. "Growth rate dependence... 2265611. PubMed Central PMCID: PMC552224. 4. Gustafsson, Claes, Sridhar Govindarajan, and Jeremy Minshull...

  16. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...Pinnelli M, Ansari R, Harper S, Jackson DA, McRae R, Pooley R, Wilkinson P, van der Meer D, Dow D, Buser-Doepner...of TSS for best results but up to 400 bp works reasonably well.  Best of luck on your experiments!  Many...Housden BE, Perrimon N, Collins JJ, Church G (2016) Comparison of Cas9 activators in multiple species. Nat Methods...doi.org/10.1038/nmeth.3871 Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes...

  17. Five Popular Model Organisms

    Type
    Blog Post
    ...cells are great model organism not only for the reasons above but because they actually share many biological... the study of neurological diseases, such as Parkinson’s disease (PD). PD is primarily characterized by...pancreas and produce different levels of insulin (Hesselson et al., 2009). The worm (Caenorhabditis elegans...nature391.6669 (1998): 806. PubMed PMID: 9486653. Hesselson, Daniel, et al. "Distinct populations of quiescent...From the baker to the bedside: yeast models of Parkinson's disease." Microbial cell 2.8 (2015): 262. PubMed...

  18. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...10.3390/antib8020028  Bird R, Hardman K, Jacobson J, Johnson S, Kaufman B, Lee S, Lee T, Pope S, Riordan... (Sandomenico et al., 2020). Figure 1: Comparison between the IgG antibody and scFv.   What...doi.org/10.1371/journal.pone.0189964  Huston JS, Levinson D, Mudgett-Hunter M, Tai MS, Novotny J, Margolies... made by molecular evolution 1 1Edited by I. A. Wilson. Journal of Molecular Biology 275:245–253 . https...

  19. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...designing and testing your AAV.  Figure 2: Comparison of AAV serotypes 1, 2, 5, 6, 8, and 9 in two ...Taschenberger et al., 2017).     Figure 3: Comparison of two astrocyte selective promoters demonstrating...Sortwell, C. E., Hauswirth, W. W., Boye, S. E., & Manfredsson, F. P. (2017). Rationally Engineered AAV Capsids...vector-mediated transduction of the corticospinal tract: Comparison of four promoters. Gene Therapy, 28(1–2), 56–... Jason Nasse...

  20. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...PMC2693335. Williams JA, Luke J, Langtry S, Anderson S, Hodgson CP, Carnes AE. Generic plasmid DNA production...and at high copy numbers (Williams, Carnes, and Hodgson, 2009). More research is ongoing to determine plasmid...90057-7. PMID: 2544913. Cook KS, Luo J, Guttman A, Thompson L. Vaccine Plasmid Topology Monitoring by Capillary...90060-6. PMID: 6323019. Williams JA, Carnes AE, Hodgson CP. Plasmid DNA vaccine vector design: impact on...

  21. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...2013), or rescue of mutant phenotypes (unc-119) (Dickinson et al., 2013). These approaches allowed effective...the development of the self-excising cassette (Dickinson et al., 2015) made this step as simple as heat-shocking...e193–e193 . https://doi.org/10.1093/nar/gkt805 Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein...sequences https://doi.org/10.17912/W2237D Farboud B, Severson AF, Meyer BJ (2018) Strategies for Efficient Genome...Krishnamurthy PM, Gneid H, Edraki A, Watts JK, Sontheimer EJ, Mello CC (2018) 5′ Modifications Improve ...

  22. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...many stages of synthesis and degradation. For this reason it is virtually impossible to determine specific...parameter to consider is the contrast ratio- a comparison of PA-FP brightness before and after photoactivation...extraordinary molecular detail.   References 1.Patterson, George H., and Jennifer Lippincott-Schwartz. ..., Samuel T., Thanu PK Girirajan, and Michael D. Mason. "Ultra-high resolution imaging by fluorescence ...Photoactivatable FPs in Empty Backbones in the Michael Davidson Collection Find Photoactivatable FPs for Single...

  23. Editor's Choice, September 2016

    Type
    Blog Post
    ...contributed by Kurt Swanson, husband of Addgene Director of Biology, Lianna Swanson, and researcher at ...in food products). I picked this post for a few reasons: 1) I think phage are super cool and have considered...proteins that could be useful for a wide variety of reasons. 3) I would love to see more posts like this on...

  24. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...use Bioluminescence Resonance Energy Transfer (BRET) were developed by the Johnson Lab . Characteristics...CRISPR labeling chromosomal loci in genome Thoru Pederson’s lab at the University of Massachusetts Medical...lab further modified sdChr- a blue-shifted channelrhodpson from freshwater green alga identified by Ed...Nano-lanterns rely on the principle of bioluminescence resonance energy transfer (BRET), whereby photons emitted...-tagged human ORF clones to allow testing and comparison of different cell-free expression systems; the...understanding complex gene regulation (Bert et al 2000, Johnson et al 2004). So if you are looking for a luciferase... et al. Plasmid. 2000. PubMed PMID: 10964627 Johnson, et al. Mol Cell Biol. 2004. PubMed PMID: 15340054...

  25. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    ... spend a few days editing and later looking for songs and sounds to synchronize with the images thereby...the hardest part of the process was to embed personality in pieces of plastic, but it’s gotten easier ...than a piece of plastic for us now). With the personality in place, everything now is about the plot. This...How You Can Use Video to Share Your Research 5 Reasons to Use Reddit for Science Communication 10 Ways...

  26. Management for Scientists: Giving Feedback

    Type
    Blog Post
    ...to improve, and they’re also clear about how the person is doing overall. Employees hate to wonder what...employee. Make sure to focus on the action, not the person - intent is usually good. Don’t pretend something...standard format not only enables you to discuss a person's performance or what has been learned, it helps... effective over time if you take it seriously.  Person who will receive feedback does a self-assessment...

  27. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ... ask a lot of questions. Personality and style conflicts are a common reason for scientists to change ...budgeting, equipment acquisition, public speaking and personnel management?  If you are thinking about a non-academic... you can help it. Observe as much as you can in person. Rotate in the lab, if possible. Attend a lab meeting...

  28. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ...   In fact, there are a lot of good reasons to stay and good reasons to go.  Here are some questions and...especially race and gender) Interpersonal issues can create interpersonal issues (one bad apple can change...

  29. The Strength of Story Telling

    Type
    Blog Post
    ...end; cause, effect; lesson, learning. Storytelling is a crucial part of interpersonal communication, which... it came time to add author details a different person in the lab wanted to be the first author. When ...protagonist will be you. Make sure to highlight what lessons you’ve learned and what knowledge you’ve gained...

  30. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    ...process samples at the picnic table before dark, a bison wandered into our campsite. We had to abandon our...our samples and move farther away until the bison meandered off. In the end we were able to safely get...get back to our lab work. Figure 3: When a bison wanders into the campsite, leave your samples behind...ThermoCas9 from the van der Oost lab. Phylogenetic comparison indicates that like GeoCas9 and ThermoCas9, IgnaviCas9...
Showing: 181 - 210 of 731 results