Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 386 results for: nans

Showing: 181 - 210 of 386 results

  1. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ... Part 1 of this series). In any case, Cre is a dominant phenotype, while you’ll want the floxed gene to...836. PubMed PMID: 29768166. Zickler, Denise, and Nancy Kleckner. "Recombination, pairing, and synapsis ...

  3. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...setting. Molecular diagnostics for the Zika virus predominantly look at viral load in patient blood and urine... carries out research in synthetic biology, nanomaterials science, and self-assembly for applications ...

  4. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...PMC84922. 5. Ugai, Hideyo, et al. "Stability of a recombinant adenoviral vector: optimization of conditions...Heidelberg, 2001. 291-306. PubMed PMID: 10634178. 8. Murnane, John P., Michael J. Yezzi, and Barbara R. Young...

  5. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...wipes) before and after your work. Keep your contaminants away While it’s likely that you’re the main ...microscope will help you identify bacterial and yeast contaminants. Set up a routine to split cells Don’t be lazy...

  6. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ... concentrations of iodixanol to separate out contaminants from an impure AAV preparation. 15%, 25%, 40%...R.A., & Samulski, R.J. (2007). Production of recombinant adeno-associated viral vectors for in vitro and...

  8. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...Figure 1). Through this method, we found key determinants of prime editing efficiency such as the length...efficiencies using sequence features and DNA repair determinants. Nature Biotechnology 2023, 1–11. https://doi.org...

  10. Tips for arabidopsis transformation

    Type
    Blog Post
    ... plant selection markers (basta, hygromycin, kananamycin, tunicamycin) (Nakagawa et al., 2007). Alternatively...at Stanford University. She studies cell fate maintenance in the stomatal lineage and has made countless...

  11. DNA Purification Without a Kit

    Type
    Blog Post
    ...into this mixture to absorb the nucleic acids. Containinants bind cellulose less tightly than nucleic acids...placing the dipstick into a wash solution removes contaminants while nucleic acids stick around. Finally, purified...

  13. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...sure that the sample is pure and free of any contaminants that could affect the measurements. You'll need...Addgene's Ready-to-Use Antibody collection Addgene's Recombinant Antibody Plasmids collection  ...

  15. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ... through Addgene. Anti-V5 [IPI-SV5-Pk1] is a recombinant version of the original V5 tag mouse monoclonal...different antibody-based applications.  IPI’s recombinant version is a fusion of mouse variable and rabbit...

  16. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...-2 research   By Ashley Waldron Ready-to-use recombinant antibodies were recently added to the Addgene...Image from Addgene. These antibodies are recombinant versions of mouse monoclonal antibodies generated...

  17. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...Kalamegam, G., Ahmed, F., Abbas, M., Sait, K., Anfinan, N., Al-Wasiyah,  mohammad khalid, Huwait, E., ...Conventional vs. Flow Cytometry Plasmid-based Recombinant Monoclonal Antibodies Introduction to Antibodies...

  18. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...fluorescent biosensors. Aptabeacons Fluorescence resonance energy transfer (FRET) technology has been studied...PMC4441765. 4. Guo, Peixuan AND Haque, Farzin. RNA Nanotechnology and Therapeutics. Boca Raton: CRC Press, 2014...

  19. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...Phenol-chloroform extraction removes remaining contaminant proteins and RNase A from the DNA sample. When... This will help to remove proteins and other contaminants from the plasmid DNA. It is also advisable to...

  20. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...several micrograms of target DNA from just a few nanograms of template DNA through several cycles of denaturation...number of µl of dH 2 O equal to the number of nanomoles of DNA times 10. For example, if your lyophilized...

  21. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ... scFvs. What is an scFv? scFVs are a type of recombinant antibody. They are ~25 kDa single polypeptides...coli Expression System in Producing Antibody Recombinant Fragments. IJMS 21:6324 . https://doi.org/10.3390...

  22. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...endogenous epitope tags, or the introduction of dominant mutations of a gene into the endogenous allele...generating human somatic cell gene knockouts using recombinant adeno‐associated viruses." Nucleic acids research...

  23. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...toolset IPI’s anti-integrin toolset comprises 26 recombinant antibodies (rAbs) either produced de novo using...an expanding collection of readily accessible recombinant antibodies and plasmids. To encourage collaboration...

  25. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...glnV44 λ- JM109   General cloning and plasmid maintenance, blue/white screening, partly restriction-deficient...(mrr-hsdRMS-mcrBC) Pir1    For cloning and maintenance of a plasmids with R6Kγ origin; contains a mutant...

  26. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...microinject into blastocysts to be implanted in pseudopregnant female mice. If you obtained the desired chimeric...microinjection, i-GONAD is performed directly on pregnant female mice using a small incision that exposes...

  27. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...doi.org/10.1371/journal.pone.0076310 Damdindorj, L., Karnan, S., Ota, A., Hossain, E., Konishi, Y., Hosokawa...Nonhuman Primates after Intramuscular Injection of Recombinant Adeno-Associated Virus. Journal of Virology, ...

  28. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...then be exposed to a lysis buffer to release recombinant proteins directly onto the nitrocellulose for... 1 M NaCl in order to enhance folding of the recombinant proteins. Optimal salt concentration will vary...
Showing: 181 - 210 of 386 results