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  1. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

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    ...nonprofit" organization what do you think of? Maybe the term brings to mind a social service organization like... about a local food pantry or community arts organization. Many people are surprised to learn that Addgene...nonprofits do with their revenue. A nonprofit organization cannot distribute profits to private individuals...as a nonprofit, we attract partnerships with organizations that share our community-oriented values. Addgenies...Meron, Michael Lo., Michael Le., Michel, Michelle, Morgan, Natashia, Nicole, Olivia, Pamela, Racheal, Ramsey...Addgene Board Member, Connie Cepko Resources on Addgene.org Learn More About Our Mission Meet the Addgene...
  2. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

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    ...specifically manipulate a cell type of interest in an organism, all cells of that type are affected. Previous...versatile and can be applied across cell types and organisms by replacing the transcription factor binding ...the PhiC31 recombinase can work in other model organisms including mouse and fish, SPARC is likely generalizable...in Drosophila. Nature Methods 9:47–55 . https://doi.org/10.1038/nmeth.1800 Isaacman-Beck J, Paik KC, Wienecke... proportions of cells. bioRxiv 788679; https://doi.org/10.1101/788679 Lin R, Wang R, Yuan J, Feng Q, Zhou...neurons. Nature Methods 15:1033–1036 . https://doi.org/10.1038/s41592-018-0184-y Additional resources...controlling gene expression in flies Resources on Addgene.org Find fluorescent protein plasmids at Addgene...
  3. Deep Dive: qPCR

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    ...fluorescent data on all channels - helpful if you forget to set the channel wavelength before you run the...14. 2017, Pages 19-28, ISSN 2214-7535, https://doi.org/10.1016/j.bdq.2017.11.001.Bustin SA; Benes V; Garson...Issue 7, 1 April 2011, Pages 1019–1020, https://doi.org/10.1093/bioinformatics/btr065Freeman WM; Walker...Reprogramming Process. Sci Rep 8, 8716 (2018). https://doi.org/10.1038/s41598-018-26707-8Suzuki T; Higgins PJ;... quality data. Sci Rep 7, 2409 (2017). https://doi.org/10.1038/s41598-017-02217-xTaylor, SC; Nadeau, K...7, 2019, Pages 761-774, SSN 0167-7799, https://doi.org/10.1016/j.tibtech.2018.12.002.Teo IA; Choi JW;  ...Issue 19, 1 October 2000, Pages 3752–3761, https://doi.org/10.1093/nar/28.19.3752Wong M; Medrano J. Real-time...
  4. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

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    ... with the Harvard GSAS Science Policy Group to organize a Minisymposium on Reproducibility. The minisymposium... additional resources and information about organizations promoting reproducibility in this booklet (which...within a published manuscript. Luckily, many organizations have been working to provide tools that make...expand to other protein technologies later. Organizations such as BenchSci and FPbase are working hard... they’ve used for data analysis while other organizations like Dryad make it easier for researchers to...are blogs - both hosted by individuals and by organizations like Addgene (a good example is our series of...editing technology NgAgo). In addition, there are organizations like ReFigure which aims to consolidate published...
  5. Antibodies 101: Multiplex Immunofluorescence

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    ... colors you like. Controls and validation Can’t forget the controls! The necessary control experiments...immunolabeling of brain. eLife, 8, e43322. https://doi.org/10.7554/eLife.43322 Brewer, M., McDonough, L., ...Fresh Frozen Tissue-V2 v2. protocols.io. https://doi.org/10.17504/protocols.io.bs68nhhw Im, K., Mareninov...1897, pp. 299–311). Springer New York. https://doi.org/10.1007/978-1-4939-8935-5_26 Jambor, H., Antonietti...publications. PLOS Biology, 19(3), e3001161. https://doi.org/10.1371/journal.pbio.3001161 McLaughlin, K. (2019...Immunohistochemistry. Materials and Methods, 9. https://doi.org/10.13070/mm.en.9.2846 Radtke, A. J., Kandov, E....Academy of Sciences, 117(52), 33455–33465. https://doi.org/10.1073/pnas.2018488117 Additional Resources on...
  6. Hot Plasmids: Spring 2025

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    ...living cells and organisms with TurboID. Nature Biotechnology, 36(9), 880–887. https://doi.org/10.1038/nbt.4201.... doi: https://doi.org/10.1016/j.neuron.2025.05.002. bioRxiv preprint: https://doi.org/10.1101/2024.07.17.603924...deactivation rates. bioRxiv, 2025.03.20.643984. https://doi.org/10.1101/2025.03.20.643984.  Build your way: Explore...activity to new DNA sites. Nature. doi: https://doi.org/10.1038/s41586-025-08877-4.  Two powerhouse tools..., B. K., Aoi, Y., Iwanaszko, M., Howard, B. C., Morgan, M. A., Andersen, G. D., Bartom, E. T., & Shilatifard... Molecular Cell, 84(24), 4929-4944.e8. https://doi.org/10.1016/j.molcel.2024.11.007. Branon, T. C., Bosch...
  7. Viral Vectors 101: Viruses as Biological Tools

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    ...easily deliver their “cargo” to target cells and organisms, and when that cargo is designed to make you sick...biological tools In nature, viruses infect cells and organisms (which we refer to as hosts) via viral particles...and fuse with. In nature, this determines which organisms and cells they infect. In the lab, this means ...can also be directly injected into hard-to-reach organs, such as the brain. Secondly, some viruses have... makes them ideal for applications with whole organisms. Finally, viral delivery is typically straightforward...strong immune response when introduced into an organism. This feature makes them an attractive tool for...Lentiviral Transduction Additional Resources on Addgene.org Browse Our Viral Vector Pages Learn About Viral...
  8. Antibodies 101: Conjugation

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    ... cytometry. Trends Immunol 33:323–332. https://doi.org/10.1016/j.it.2012.02.010 Dennler P, Fischer E, ...Defined Reagents. Antibodies 4:197–224. https://doi.org/10.3390/antib4030197  Hegazy M, Cohen-Barak E, ...in Situ. Curr Protoc Cell Biol 89:e115. https://doi.org/10.1002/cpcb.115  Mueller BM, WrasidloO WA, Reisfeld...Monoclonal Antibodies. Hybridoma 7:453–456. https://doi.org/10.1089/hyb.1988.7.453  Niemeyer CM, Adler M, Wacker...quantitative immuno-PCR. Nat Protoc 2:1918–1930. https://doi.org/10.1038/nprot.2007.267 Stoeckius M, Hafemeister... single cells. Nat Methods 14:865–868. https://doi.org/10.1038/nmeth.4380  Additional resources on the...BlottingPlasmids 101: Biotinylation Resources on Addgene.org Unconjugated antibodies at AddgeneAntibody plasmids...
  9. RNA Extraction Without A Kit

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    ...proteins are retained in the interphase and bottom, organic layer. The top aqueous layer is extracted and RNA...layer to avoid carryover of DNA from the bottom, organic layer. Another trick is to precipitate the RNA ...Biophysica Acta (BBA) - Enzymology 341:484–488. https://doi.org/10.1016/0005-2744(74)90240-x Chirgwin JM, Przybyla...ribonuclease. Biochemistry 18:5294–5299. https://doi.org/10.1021/bi00591a005 Chomczynski P, Sacchi N (1987.... Analytical Biochemistry 162:156–159. https://doi.org/10.1016/0003-2697(87)90021-2 Chomczynski P, Sacchi... years on. Nature Protocols 1:581–585. https://doi.org/10.1038/nprot.2006.83 Additional resources on the...for purifying DNA without a kit Resources on Addgene.org Check out our molecular biology protocols Find...
  10. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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    ...In 2017, Lenny Teytelman, CEO of protocols.io, organized a panel to discuss reproducibility issues in research... there were other academics and like-minded organizations with similar ideas who were also thinking about... an eLife ambassador, had previous experience organizing open access events and grassroots efforts. After...in developing reproducibility workshops and organizational skills. Meanwhile, Addgene was also thinking... in the year and in the fall of 2018, I helped organize a workshop for community scientists at the Global... would want to know before, during, and after organizing such a workshop. Finally, the group put a lot...IS-MPMI. If you’re interested in having a workshop organized at your upcoming conference or you’d like to join...
  11. Working with Nuclear Receptors

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    ...McKenna and Bert W. O’Malley. Survival of all organisms depends on efficient energy maintenance - through...nervous system signaling to peripheral effector organs, which then return information such as nutritional...processes are regulated at the cellular, tissue and organ level by nuclear receptors (NRs), their coregulators... of such signals between central and peripheral organs because of their established roles as molecular...activation or repression of gene expression (see www.nursa.org for a curated list of coregulators). Coregulators...context defined by ligand, cell type, and promoter, organizing the expression of functional groups of genes ...Nuclear Receptor Signaling Atlas (NURSA) at www.nursa.org. Bert O’Malley, M.D., is Tom Thompson Professor...
  12. Antibodies 101: Introduction to Antibodies

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    ...cells or tissue slices mounted to a slide, or whole organs. Because the tissue architecture is preserved, ...from the bloodstream. Then, via a blood draw or organ harvest, the labeled cells can be analyzed using...and Future Outlook. Front Immunol 8: . https://doi.org/10.3389/fimmu.2017.01589 Ayyar BV, Arora S, Ravi...The nuts and bolts. Methods 116:51–62 . https://doi.org/10.1016/j.ymeth.2017.01.009 Crivianu-Gaita V, ...Biosensors and Bioelectronics 85:32–45 . https://doi.org/10.1016/j.bios.2016.04.091 Cruse JM, Lewis RE,...Immunology Guidebook. Elsevier, pp 17–45. https://doi.org/10.1016/B978-012198382-6/50026-1 Wang R, Xiang ... Skp. Front Cell Infect Microbiol 3: . https://doi.org/10.3389/fcimb.2013.00072 Additional resources on...
  13. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    ...Girardia sp., Dugesia dorotocephala, Phagocata morgani, and Phagocata gracillis. There are 11 different...developmental biologists have to extract RNA from their organism of interest, use the RNA to create a complementary... in  the labeling specific cell types or entire organs in planarians. For example, the gene Piwi labels...strain that is designated as DE3. For Phagocata morgani and Phagocata gracillis, the process is a little...E. coli. Thus, to get the dsRNA into Phagocata morgani and Phagocata gracillis, one has to purify the ..., feedback etc. please write to cuttingclass@stowers.org and if you make new plasmids for investigating...Models for learning about CRISPR Resources at Addgene.org Find plasmids from the Sánchez lab Find plasmids...
  14. Plasmids 101: Modular Cloning

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    ... 179 (2020). https://doi.org/10.1186/s12870-020-02388-2 Resources on Addgene.org Addgene’s Modular Cloning...modular cloning? Modular cloning, or MoClo, is an organization scheme for multi-part DNA assembly. Simply put...’s because MoClo specifically refers to the organizational schema described above, and not any particular...other non-Golden Gate cloning methods. MoClo’s organization schema MoClo can be broken down into three hierarchical...many guides/parts delivered) in certain model organisms. In mammals, most CRISPR libraries are assembled...targeting constructs and delivering them to these organisms via another optimized platform. Gene Stacking ...
  15. The AAVantages of AAV in CRISPR Screens

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    ...screens. Nature Protocols, 17(9), 1903–1925. https://doi.org/10.1038/s41596-022-00700-y Hanlon, K. S., Kleinstiver... breaks. Nature Communications, 10(1). https://doi.org/10.1038/s41467-019-12449-2 Pupo, A., Fernández,... Molecular Therapy, 30(12), 3515–3541. https://doi.org/10.1016/j.ymthe.2022.09.015 Ramani, B., Rose, I...bioRxiv (Cold Spring Harbor Laboratory). https://doi.org/10.1101/2023.06.13.544831 Santinha, A. J., Klingler...AAV-Perturb-seq. Nature, 622(7982), 367–375. https://doi.org/10.1038/s41586-023-06570-y Wang, J., Gessler, D...Transduction and Targeted Therapy, 9(1). https://doi.org/10.1038/s41392-024-01780-w Additional resources...Using the FITS Approach Additional resources at addgene.org CRISPR pooled libraries Guide to pooled libraries...
  16. Common Injection Routes in Mice

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    ... available in your lab or organization, and approvals from the organization overseeing your work. Good...Modeling Part 2 Five Popular Model Organisms Additional resources on Addgene.org Genome Engineering Guide CRISPR...the peritoneal cavity, or the space surrounding organs in the abdomen. They are a popular choice for administering...and to avoid potential off-target pokes to nearby organs. Restraint: scruffing Location: peritoneal cavity...Biosciences and Medicines, 08(09), 131–141. https://doi.org/10.4236/jbm.2020.89011 Shimizu, S. S. (2004). Routes...
  17. Experimenting with New Careers while in Grad School

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    ...educating the public and organizing events. I contacted local professional organizations and nonprofits. I volunteered...Central Indiana Science Outreach, or CINSO. We organize fun science events for adults and professional...communication, editing, marketing, and website organization. My short experience with my blog helped immensely...establishments such as museums, professional organizations, and other nonprofits to gain support for CINSO...read from career guidance sources such as myidp.sciencecareers.org mentioned museums. Indianapolis is home...Browse All of Our Career Posts Resources on the Addgene.org Find Plasmids for Your Research Learn About ...
  18. Transferable Skills Guide: Identifying Your Transferable Skills

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    ...your organization Public Speaking Time management Managing a team Conflict resolution Organization Leadership... given yourself the bathroom mirror pep talk, forgotten and then retrieved your lucky socks, persevered...than you thought! When change happens at your organization, when your boss needs someone to step up, when...likely collected some great time management and organizational skills. If you are responsible for working ...students, managing inventories in the lab, or organizing a research project, one can assume that you’ve... careers Learn about mentoring Resources on Addgene.org Check out our careers page Watch our career ...
  19. A Career in Grants: How To Become a Grant Professional

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    ...professional works for or with an organization to evaluate the organization’s grant readiness, identify grant...stories about a time when their passion for an organization or a program and a funding opportunity coincided...submitting it, to evaluating the capacity of an organization to receive and manage funds.  Grant professionals...targeted to the grants profession? The number of organizations, both public and private, that qualify for grants...oriented nonprofits. Like Deb, I worked for an organization in a position that required some involvement...your ideal client or job will save you and the organization(s) you serve time and resources. 3. Get a mentor...
  20. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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    ...into the results, it’s important to note that in Morgens et al., guides were considered toxic if they were...non-specific toxicity than non-targeting guides First, Morgens et al. looked at the effect of safe-targeting guides...guides. The genome-wide CRISPR screen presented in Morgens et al. contains three types of guides: 1) targeting... reduction in on-target efficacy. Results from Morgens et al. show that genome-wide CRISPR screens are...testing thousands of guides for off-target activity, Morgens et al drew some generalizable conclusions about...Library are available from Addgene! References 1. Morgens, David W., et al. "Genome-scale measurement of ...Guide to All Things Lentivirus Resources on Addgene.org Check out this guide to pooled plasmid libraries...
  21. Hot Plasmids: Fall 2024

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    ...Anti-V5 [IPI-SV5-Pk1]. Addgene Report, https://doi.org/10.57733/addgene.l0ulu4. Hanke, T., Szawlowski,...antigen. J Gen Virol., 73 (Pt 3), 653–660. https://doi.org/10.1099/0022-1317-73-3-653. PMID 1372038. Randall.... J Gen Virol., 68 (Pt 11), 2769–2780. https://doi.org/10.1099/0022-1317-68-11-2769. PMID 2445904.   ...protein degraders. Nat Commun., 15(1), 8885. https://doi.org/10.1038/s41467-024-52871-9. PMID 39406745. Optimizing...Caenorhabditis elegans. Genetics, 228(2), iyae126. https://doi.org/10.1093/genetics/iyae126.   Gene disruption in...abscessus. J Bacteriol., 206(3), e0033523. https://doi.org/10.1128/jb.00335-23.  Bioluminescent reporting...Systems. ACS Sensors, 9(7), 3489–3495. https://doi.org/10.1021/acssensors.3c02664. ...
  22. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    ...without DNA cleavage. Nature 551:464–471 . https://doi.org/10.1038/nature24644 Glaser A, McColl B, Vadolas...Molecular Therapy - Nucleic Acids 5:e334 . https://doi.org/10.1038/mtna.2016.48 Katti A, Foronda M, Zimmerman... Nucleic Acids Research 48:2841–2852 . https://doi.org/10.1093/nar/gkaa124 Martin AS, Salamango DJ, Serebrenik...editosome complexes. Scientific Reports 9: . https://doi.org/10.1038/s41598-018-36739-9 St. Martin A, Salamango...cells. Nucleic Acids Research 46:e84–e84 . https://doi.org/10.1093/nar/gky332 Standage-Beier K, Tekel SJ, ... Nucleic Acids Research 47:e120–e120 . https://doi.org/10.1093/nar/gkz713 Additional resources on the ...reporters Browse our CRISPR blog posts Resources on Addgene.org Browse the CRISPR guide Find CRISPR plasmids...
  23. Tips for Writing a Good Cover Letter

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    ...asks for strong organizational skills. You might think, “I’d be good at that! I organized my lab data so...important part of the application. Even within an organization, there can be variance. At Addgene, our content...explain why you are interested in working for that organization or group. This is particularly important for...want to talk about! You could describe your organizational system, your rationale for designing it the...title of the position] at [name of the company/organization]. I am a [adjective that describes you] scientist...that particular job, your commitment to the organization's mission, or your deep interest in the lab's...
  24. Using Video to Share Your Science: We Share Science

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    ...Watkins, Professor at George Washington University and developer of wesharescience.org. Sharing your research...professor at George Washington University and he the developer of the WeShareScience.org platform. You...study processes for diagnosing and resolving organizational performance and often have to be able to quickly...sharing video abstracts about research: www.WeShareScience.org. WeShareScience is a free platform for ...Sharing in Research Publications Resources at Addgene.org Check out our Protocol Pages for Video Protocols...
  25. Antibody Validation for Flow Cytometry

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    ...efforts and information provided by external organizations like the Human Cell Differentiation Molecules...research applications. eLife, 12, RP91645. https://doi.org/10.7554/eLife.91645 Biddle, M. S., Alende, C., ...flow cytometry. F1000Research, 13, 817. https://doi.org/10.12688/f1000research.154034.1 Laflamme, C., McKeever...disease gene C9ORF72. eLife, 8, e48363. https://doi.org/10.7554/eLife.48363 Uhlen, M., Bandrowski, A., ...antibodies. Nature Methods, 13(10), 823–827. https://doi.org/10.1038/nmeth.3995 Virk, H. S., Biddle, M. S., ...Journal of Pharmacology, 178(15), 2948–2962. https://doi.org/10.1111/bph.15467 Virk, H. S., Rekas, M. Z., Biddle...TRPA1. Scientific Reports, 9(1), 18500. https://doi.org/10.1038/s41598-019-55133-7 More resources from ...
  26. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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    ...detection and identification using FnCas9. https://doi.org/10.1101/2020.04.07.028167 Broughton JP, Deng X,...detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0513-4 Kellner MJ, Koob JG, ...nucleases. Nature Protocols 14:2986–3012 . https://doi.org/10.1038/s41596-019-0210-2 Lucia, C., Federico, ...-Cas12. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.02.29.971127 Rauch, J. N., Valois,...Material. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.20.052159 Zhang, F., Abudayyeh,...COVID-19 using CRISPR diagnostics. https://www.broadinstitute.org/files/publications/special/COVID-19%20detection...available for COVID-19 research Resources on Addgene.org Get the basics about CRISPR in our CRISPR guide...
  27. Hot Plasmids: Summer 2024

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    ...samples. bioRxiv 2024.01.21.576499; doi: https://doi.org/10.1101/2024.01.21.576499.    LEA proteins protect...freezing. bioRxiv 2024.02.06.579238. doi: https://doi.org/10.1101/2024.02.06.579238.    Engineered and evolved...Biotechnology, 10.1038/s41587-023-02078-y. doi: https://doi.org/10.1038/s41587-023-02078-y.  Anzalone, A. V., Gao...Nature Biotechnology, 40(5), 731–740. doi: https://doi.org/10.1038/s41587-021-01133-w Pandey, S., Gao, X. ...Engineering, 10.1038/s41551-024-01227-1. doi:  https://doi.org/10.1038/s41551-024-01227-1   CHARM: A compact ...editor. Science, 384(6703), ado7082. doi: https://doi.org/10.1126/science.ado7082.  Nuñez, J. K., Chen, J...editing. Cell, 184(9), 2503–2519.e17. doi: https://doi.org/10.1016/j.cell.2021.03.025.    Recombinant antibodies...
  28. Our Most Popular Blog Posts from 2019

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    ...Popular Model Organisms by Alyssa Cecchetelli and Lukas Morgan There are many model organisms people are...interview including defining your research vision, organizing your vision into a chalk talk, and tips for practicing.... In this blog post, we’ve covered five model organisms: mouse, fruit fly, yeast, zebrafish, and worm.... by leaving a comment or emailing us at blog@addgene.org.  Thank you again for a fantastic year and happy...
  29. Science as Art

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    ...to me… The problem is that scientists sometimes forget how to communicate about their work with non-academic... is essential to stay in touch with the living organism I study, to be able to see it all scales. Most... a way to better understand and appreciate the organism, for both scientist and non-scientist alike. “...inner structures of delicate plants and other organisms without destroying the original, which could provide...Do you look for the beauty in the microscopic organisms on your lab bench? Let us know in the comments...Experimenting in an Open Source Lab Resources on Addgene.org Try the Plasmid Land Game ...
  30. Exploring Applications of the Bioluminescent HiBiT Tag

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    ...ACS Chemical Biology, 7(11), 1848–1857. https://doi.org/10.1021/cb3002478 Kamada, Y., Tateishi, H., Nakayamada...of Misfolded CFTR. Cells, 12(23), 2741. https://doi.org/10.3390/cells12232741  Kamio, T., Kono, Y., Hirosuna...Gastric Cancer. Cancer Medicine, 14(8). https://doi.org/10.1002/cam4.70793 Laissue, P. P., Alghamdi, R....imaging. Nature Methods, 14(7), 657–661. https://doi.org/10.1038/nmeth.4344  Peach, C. J., Kilpatrick, L...Journal of Pharmacology, 178(12), 2393–2411. https://doi.org/10.1111/bph.15426 Promega Corporation (2025). Illuminating...and in vitro. Oncology Reports, 50(5). https://doi.org/10.3892/or.2023.8631 Tsien R. Y. (1998). The green...Annual Review of Biochemistry, 67, 509–544. https://doi.org/10.1146/annurev.biochem.67.1.509  More resources...
  31. Antibodies 101: The Basics of Western Blotting

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    ...analyses using ELISA. Peptides 72:4–15 . https://doi.org/10.1016/j.peptides.2015.04.012 Burnette WN (1981... Analytical Biochemistry 112:195–203 . https://doi.org/10.1016/0003-2697(81)90281-5 Pillai-Kastoori L,...for the user. J Biol Chem 295:926–939 . https://doi.org/10.1074/jbc.ra119.010472 Renart J, Reiser J, Stark...National Academy of Sciences 76:3116–3120 . https://doi.org/10.1073/pnas.76.7.3116 Towbin H, Staehelin T, Gordon...National Academy of Sciences 76:4350–4354 . https://doi.org/10.1073/pnas.76.9.4350 Yang P-C, Mahmood T (2012...trouble shooting. North Am J Med Sci 4:429 . https://doi.org/10.4103/1947-2714.100998 Additional resources on...articles related to antibodies Resources on Addgene.org Find written and video protocols Visit Addgene's...
  32. The Golden Stopwatch Award: Recognizing Facilitators of Research

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    ...here yet? A quick look at your order status on addgene.org identifies the hold-up. Your Material Transfer...possible Tell them about the Golden Stopwatch! Any organization that deposits with and/or requests materials...to Addgene’s Tech Transfer Team: techtransfer@addgene.org Congratulations to the Golden Stopwatch awardees...to all of the winners so far! Don’t see your organization below? Don’t worry--we check periodically for...transfer offices. Drop us a line at techtransfer@addgene.org. Albert Einstein College of Medicine  Baylor...of commercialization Additional resources on Addgene.org Addgene Help Center: Tech Transfer Technology...
  33. CRISPR 101: Non-Homologous End Joining

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    ...Mutagenic Potential. PLoS Genet 5:e1000683 . https://doi.org/10.1371/journal.pgen.1000683 Bétermier M, Bertrand...Error-Prone Process? PLoS Genet 10:e1004086 . https://doi.org/10.1371/journal.pgen.1004086 Ghezraoui H, Piganeau...End-Joining. Molecular Cell 55:829–842 . https://doi.org/10.1016/j.molcel.2014.08.002 Ran FA, Hsu PD, Lin...Editing Specificity. Cell 154:1380–1389 . https://doi.org/10.1016/j.cell.2013.08.021 Waters CA, Strande NT...nonhomologous end joining. Nat Commun 5: . https://doi.org/10.1038/ncomms5286 Yan M-Y, Li S-S, Ding X-Y, Guo...Mycobacterium tuberculosis. mBio 11: . https://doi.org/10.1128/mbio.02364-19 More CRISPR Blog Posts from...
  34. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    ...modulating SARS-CoV-2 infection. bioRxiv. https://doi.org/10.1101/2021.04.22.440848 Crawford KHD, Eguia R...Neutralization Assays. Viruses 12:513. https://doi.org/10.3390/v12050513 Hsieh C-L, Goldsmith JA, Schaub...SARS-CoV-2 spikes. Science 369:1501–1505. https://doi.org/10.1126/science.abd0826 Tong P, Gautam A, Windsor... of evolving SARS-CoV-2 spike bioRxiv. https://doi.org/10.1101/2021.03.10.434840 Tsang J, LaManna CM (...Detection Tools. The CRISPR Journal 3:142–145. https://doi.org/10.1089/crispr.2020.0030 Additional resources on...Browse all COVID-19 blog posts Resources on Addgene.org Find COVID-19 resources on our COVID-19 and ...
  35. Plasmids for Endogenous Gene Tagging in Human Cells

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    ...Cell Explorer at allencell.org and the Allen Institute website at alleninstitute.org. A classic challenge...Cell Explorer at allencell.org and the Allen Institute website at alleninstitute.org. References 1. Roberts.../Cas9 in human stem cells to illuminate cell organization." bioRxiv (2017): 123042. Additional Resources... Our Fluorescent Protein Posts Resources on Addgene.org Browse CRISPR Tagging Plasmids Browse the Fluorescent...
  36. Design Tips for Prime Editing

    Type
    Blog Post
    ...donor DNA. Nature, 576(7785), 149–157. https://doi.org/10.1038/s41586-019-1711-4 Chen, P. J., Hussmann...outcomes. Cell, 184(22), 5635-5652.e29. https://doi.org/10.1016/j.cell.2021.09.018 Nelson, J. W., Randolph... Nature Biotechnology, 40(3), 402–410. https://doi.org/10.1038/s41587-021-01039-7 Nishimasu, H., Ran, ...and Target DNA. Cell, 156(5), 935–949. https://doi.org/10.1016/j.cell.2014.02.001 Yan, J., Oyler-Castrillo...RNA-binding protein. Nature, 628(8008), 639–647. https://doi.org/10.1038/s41586-024-07259-6 Additional Resources... Download the CRISPR 101 eBook Resources on Addgene.org Read the CRISPR guide Find CRISPR plasmids by...
  37. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ... https://wiki.hackerspaces.org/Hackerspaces OpenWorm Project Website: http://www.openworm.org/ Cat Microbiome...1,000 active makerspaces around the world. Hackerspaces.org maintains a list of active spaces near you...While C. elegans are often thought of as simple organisms , they have many levels of complexity, especially...collection of samples and sequencing is being organized and funded by someone else (KittyBiome has been...University at NASA Ames, and an instructor at Biocurious.org, Derek is bringing cutting edge technologies...
  38. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...result of the content. Here are some of my tips to organizing and running a memorable panel discussion. Check...make it look effortless to the audience, but the organizers and moderator should have a well-planned approach...panelists to the moderator. The moderator and organizers should develop a list of 4-6 pre-event interview...interview questions. The moderator (possibly with an organizer) should set up a 30 minute call with each panelist...example, at a recent event on nonprofit science organizations, I asked audience members to stand and describe...networking time. Both the moderator and program organizers can help make sure the event stays on schedule...
  39. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...e-mail us at  deposit@addgene.org Special cases Please contact us at deposit@addgene.org or +1 (617)-ADDGENE...plasmids, please let us know by contacting deposit@addgene.org. These deposits are considered large deposits.... Email the completed spreadsheet to deposit@addgene.org along with your GenBank files, plasmid sequences...library with Addgene?" or contact us at deposit@addgene.org or +1 (617)-ADDGENE (233-4363) with additional...to your strain. Please contact us at deposit@addgene.org or +1 (617)-ADDGENE (233-4363) if you’d like ...after submission, please email us at deposit@addgene.org. Include your deposit number and which data needs...
  40. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...1513. doi: https://doi.org/10.1038/s41477-024-01786-w. (bioRxiv preprint: https://doi.org/10.1101/2024.02.11.579803...cargo. bioRxiv 2024.12.20.629623; doi: https://doi.org/10.1101/2024.12.20.629623.  A fast and responsive...events. Neuron, 112(22), 3680–3696.e8. doi: https://doi.org/10.1016/j.neuron.2024.08.019.  ONE-GO biosensors...platform. Cell, 187(6), 1527–1546.e25. doi: https://doi.org/10.1016/j.cell.2024.01.028. New recombinant antibodies...Immunohistochemistry (Human). Addgene Report, doi: https://doi.org/10.57733/addgene.6ncijt.  ...
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