We narrowed to 389 results for: ARIA

1. Working with Nuclear Receptors

   Type

   Blog Post

   ...‘core’ complex is composed of a tightly-bound invariant group of proteins, whereas the more loosely bound...

2. Antibody Guide

   Type

   Guide

   ...Single-chain fragment variables (scFvs) contain only the variable heavy chain and variable light chain regions... way to produce antibodies, with the highest variability in antibodies produced. Cells - Antibodies can...large amounts of a single antibody with little variation. Recombinant plasmids - Antibodies can be produced...the plasmid. This produces the least amount of variation between antibodies, allows for the antibody’s ...easily confirmed via sequencing, and enables the variable region and constant regions of the antibody to.... The tips of the Fab fragments contain highly variable regions, shown in light blue and light orange,...covered in the following Isotypes section. The variable region of the antibody is the antigen binding ...

3. Plasmids 101: Modular Cloning

   Type

   Blog Post

   ... allows researchers to assemble and test many variables all at once. CRISPR CRISPR arrays and libraries...

4. Fluorescent Proteins 101: Introduction to FRET

   Type

   Blog Post

   ...originally described by Theodor Förster in 1948 as a variation of the more commonly observed light emission by...

5. High Complexity Golden Gate Assembly with NEB’s DAD

   Type

   Blog Post

   ... to 49% fidelity, with significant lot-to-lot variation. Additionally, this assembly, which used only ...

6. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

   Type

   Blog Post

   ...then mutagenize the NFIX gene and test whether variants of unknown significance (VUSs) that are frequently...

7. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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   Blog Post

   ... stability allowing for minimized basal FRET variability, and is a good choice for FRET assays where a...

8. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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   Blog Post

   ... the pool but instead selectively sequence the variable portion of the plasmid in the pool - ex. the gRNA...

9. Hot Plasmids - October 2020

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   Blog Post

   ...in worms than split-sfCherry3. It was created by Maria Ingaramo, Jerome Goudeau, Cynthia Kenyon, and colleagues...

10. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type

    Blog Post

    ...CRISPR activation uses dCas9, a CRISPR protein variant lacking its endonuclease ability, to bind to genes...

11. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type

    Blog Post

    ...multitude of known AAV serotypes that have slight variations in their viral capsids. Novel forms of AAV capsids...

12. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    ...Cpf1 is also sensitive to single mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1...

13. Plasmids 101: Terminators and PolyA signals

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    Blog Post

    ...important role in RNA processing and contributes to variability in RNA half-life, and ultimately gene-expression...

14. Illuminating Epigenetics with A FRET Based Biosensor

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    Blog Post

    ...transposon methylation underlies the mantled somaclonal variant of oil palm." Nature 525.7570 (2015): 533-537. ...

15. Hot Plasmids: Winter 2025

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    Blog Post

    ...improved kinetics and responsivity in screening ASAP3 variants. B) Electrical and optical recordings of spontaneous...

16. The AAVantages of AAV in CRISPR Screens

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    Blog Post

    ...the amount of cloning, another option is to use variants of Cas9, like split Cas9, or a smaller Cas9 ortholog...

17. Production of Virus in Insect Versus Mammalian Cells

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    Blog Post

    ...are Sf9 cells, which are derived from the pupa ovarian tissue of fall armyworms (Spodoptera frugiperda...

18. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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    Blog Post

    ...sometimes limited across different targets due to variations in chromatin context. Our recent studies (Mathis...

19. 3D Printing Meets CRISPR Cas9

    Type

    Blog Post

    ... this interrogation process, they will almost invariably say something like “Oh,…I didn’t quite appreciate...

20. Cloning Mammalian Cells with the Agarose Method

    Type

    Blog Post

    ...recommend the use of an electronic pipettor with variable spacing (such as the Viaflo from Integra Biosciences...

21. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type

    Blog Post

    ... to avoid unmixing issues from batch-to-batch variation. Tandem dyes (two fluorescent molecules conjugated...

22. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type

    Blog Post

    ...MOI optimization to account for batch-to-batch variability. Check out this post to learn more about different...

23. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type

    Blog Post

    ...that worked with NGS data were: Crispresso CrispRVariants AmpliCan We chose to adapt Crispresso by Luca...

24. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type

    Blog Post

    ...the thousands of other scientists using pLKO.1 variants learn about those updates? This is a problem many...

25. Great Results Start with Great Standard Curves

    Type

    Blog Post

    ...allows you to calculate the curve’s coefficient of variation, or %CV. The %CV of a sample is the standard deviation...

26. Antibodies 101: Producing Recombinant Antibodies

    Type

    Blog Post

    ...used for the production of Fab fragments where variable chains are secreted into the periplasmic space...

27. An Introduction to Adenovirus

    Type

    Blog Post

    ...2007-02-062117. PMID: 17510320. Tollefson, A. E., Scaria, A., Hermiston, T. W., Ryerse, J. S., Wold, L. ...

28. AAVs in Retinal Gene Therapy

    Type

    Blog Post

    ...improve the technology: engineering of new capsid variants, designing expression cassettes to regulate and...

29. A Guide to Starting Your Own Journal Club

    Type

    Blog Post

    ...welcome to ask any questions they have.  These variations are suited for different applications and should...

30. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type

    Blog Post

    ...BiFC experiments. The ideal control is a mutated variant of one or both of your PIPs in which the protein-interaction...

31. Negative Can Be Positive: Open AAV Data with Addgene

    Type

    Blog Post

    ...Boulis, N. M., & Donsante, A. (2019). Lot-to-Lot Variation in Adeno-Associated Virus Serotype 9 (AAV9) Preparations...

32. Summer SciComm Series: Modes of Communication

    Type

    Blog Post

    ...come to you (a TikTok scicomm account aimed at octogenarians is going to be a hard sell!) Digital platforms...

33. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type

    Blog Post

    ...systems consist of a genetically encoded enzyme variant tag that reacts specifically and irreversibly with...

34. Cpf1: A New Tool for CRISPR Genome Editing

    Type

    Blog Post

    ...Cpf1 is also sensitive to single mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1...

35. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type

    Blog Post

    ...within each color also exist with only slight variations separating them.  Please note that many fluorescent...

36. QC Sequencing Technologies at Addgene

    Type

    Blog Post

    ...Addgene have minor differences due to in silico variations and lack of verification. To address this issue...

37. CRISPR 101: Any Base Transversion Editors

    Type

    Blog Post

    ...models-assisted optimization of a uracil-N-glycosylase variant enables programmable T-to-G and T-to-C base editing...

38. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type

    Blog Post

    ...save researchers significant time and removes a variable from the lengthy process of validating and using...

39. Hot Plasmids Spring 2024

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    Blog Post

    ...domain conferred several new improvements. The new variant has increased signal-to-noise ratio and better ...

40. Plasmids 101: Broad Host Range Plasmids

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    Blog Post

    ...structural elements that are versatile and adaptable. Variation in ori structure allows plasmids to utilize diverse...

41. Plasmids 101: Gateway Cloning

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    Blog Post

    ... Maria Soriano...

42. Viral Vectors 101: Preparing Pooled Libraries

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    ...types and generally requires optimization of many variables, such as seeding density and reagent concentration...

43. Neuronal labeling with Spaghetti Monster

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    Blog Post

    ...detection: fluorescent protein structure and color variants." Cold Spring Harbor Protocols 2009.12 (2009):...

44. Analyzing CRISPR Editing Results with ICE from Synthego

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    ...featured topic page xCas9: Engineering a CRISPR Variant with Pam Flexibility RNA Editing with Cas13 and...

45. Antibodies 101: Immunoprecipitation

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    Blog Post

    ... of interest on the C- or N-terminus. In this variation, called a pull-down assay, an antibody against...

46. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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    Blog Post

    ...genetic tools currently for terrestrial frogs. Cnidarian-Dinoflagellate symbionts (Virginia Weis) Sea anemone...

47. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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    Blog Post

    ...the Mentor-Singleton relationship. Some of the variability was organic, as some people need less assistance...

48. Overcoming the AAV Size Limitation for CRISPR Delivery

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    Blog Post

    ...Resources on the Addgene Blog Learn more about Cas9 Variants and the Expanded CRISPR Toolbox Read about CRISPR...

49. Supporting Reproducibility with a Connected ELN

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    Blog Post

    ...Wisconsin in 2011/12 – researchers and PIs, science librarians and data curators, IT managers, the commercialization...

50. AAV Titers: Where Do They Come From And What Do They Mean?

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    Blog Post

    ...expression analysis with low abundant targets: from variable nonsense to publication quality data." Scientific...