We narrowed to 438 results for: ERG

1. A Deep Dive into BioBuilder

   Type

   Blog Post

   ... communication. And as a program focused on an emerging scientific field, BioBuilder embraces new opportunities...

2. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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   Blog Post

   ...High-throughput Phenotyping of Lung Cancer Somatic Mutations. Berger AH, Brooks AN, Wu X, et al. Cancer Cell, 2016. ...

3. Multiplex Genome Editing with CRISPR-Cpf1

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   Blog Post

   ...31-34. Pubmed PMID: 27918548. 2. Zetsche, B., Gootenberg, J., Abudayyeh, O., Slaymaker, I., Makarova, ...

4. Screening for Successful Genome Editing with Digital PCR

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   Blog Post

   ...this blog post, we’ll introduce digital PCR as an emerging validation technology. Digital PCR has several...

5. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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   Blog Post

   ...the endogenous tRNA-processing system. Xie K, Minkenberg B, Yang Y. Proc Natl Acad Sci U S A. 2015 Mar...

6. 3 Challenges in Plant Synthetic Biology

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   Blog Post

   ...and anger directed at GM crops has burdened this emerging engineering technology. Monoculture and chemical...

7. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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   Blog Post

   ...safety net" at the VC firm and devoted 100% of his energy and effort into making the startup self-sustainable...

8. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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   Blog Post

   ...meetings a scheduling priority (and last minute lab emergencies are no excuse).  Assign each meeting a leader...

9. Viral Vectors 101: An Introduction to AAV

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   Blog Post

   ... therapy 8.16 (2001). PubMed PMID: 11509958. Vandenberghe, Luk H., et al. "Efficient serotype-dependent...

10. To Each HIS Own

    Type

    Blog Post

    ...Martynoff G, Lou J, Hipskind RA, Nordheim A, Stunnenberg HG. Rapid and efficient purification of native...

11. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    Blog Post

    ...including conversion of CO2 into water and electrical energy generation. Alistair McCormick’s lab recently developed...

12. What's New in CRISPR - November 2021

    Type

    Blog Post

    ... the prime editor, yielding PEmax, to work synergistically with epegRNAs and PE4 and PE5. These enhanced...

13. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type

    Blog Post

    ...up applications to research technicians and undergraduate researchers, in addition to postdocs and graduate...

14. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type

    Blog Post

    ...of plasmids to study PPI using Förster Resonance Energy Transfer (FRET, Heckler et al., 2015) or ratiometric...

15. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type

    Blog Post

    ...transcription. Without TKA1, cells proliferate and cannot undergo apoptosis, thus multiplying uncontrollably and ...

16. CRISPR 101: Cas9 vs. The Other Cas(s)

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    Blog Post

    ...10.1016/j.molcel.2019.03.014 Zetsche, Bernd, HGootenberg, S., Jonathan, Abudayyeh, O., Omar, et. al. Cpf1...

17. Giving gRNAs a Facelift - Synthetic and Beyond

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    Blog Post

    ...https://doi/pdf/10.1021/jacs.2c02633 Allen, D., Rosenberg, M., Hendel, A. Using Synthetically Engineered...

18. Summer SciComm: Preprints

    Type

    Blog Post

    ...for researchers to share information about the emerging COVID-19 pandemic quickly and openly. By sharing...

19. Summer SciComm Series: Cognitive Load

    Type

    Blog Post

    ...field to read this paper than it would be for an undergrad taking their first upper-level class. Even so,...

20. Click Biology: How to Click and Play to Explore Biology

    Type

    Blog Post

    ... Addgene Link Click Biology Tag Description Humberg et al. (2025) Split inteins Cystein-less, fast ...

21. Human Germline Editing Using CRISPR

    Type

    Blog Post

    ...of this paper is the failure of the embryos to undergo editing using the provided ssODN template. In fact...

22. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type

    Blog Post

    ...-4 were modified from this reference. 2. Regot, Sergi, Jacob J. Hughey, Bryce T. Bajar, Silvia Carrasco...

23. Working with Nuclear Receptors

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    Blog Post

    ... Survival of all organisms depends on efficient energy maintenance - through acquisition, storage, and...

24. Early Career Researcher Toolbox: Finding Relevant Papers

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    Blog Post

    ...literature discovery platform developed by the Chan Zuckerberg Initiative. Meta is not a traditional search ...

25. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    Blog Post

    ...BM, Griffths A, Seaman MS, Schmidt AG, Chen B, Neuberg D, Bajic G, Harrison SC, Wesemann DR (2021) Memory...

26. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    Blog Post

    ...doi.org/10.1089/hum.2019.277 Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD, Sanjana...

27. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type

    Blog Post

    ...knockout mice. Knockout mice Knockout mice have undergone genetic modification that alters or eliminates...

28. Hot Plasmids - October 2020

    Type

    Blog Post

    ...examining the ratio of blue to red cells. Cells undergoing rapid cell cycles have a high blue to red ratio...

29. Popular Retroviral Vectors and Their Uses in Scientific Research

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    Blog Post

    ... target genes. This empty MuLV vector from the Weinberg lab enables researchers to express siRNAs of their...

30. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    ...NFIX gene. These diseases are characterized by overgrowth phenotypes and neural developmental delay. Mutations...

31. A Look at Addgene's QC Process

    Type

    Blog Post

    ...Once Addgene receives deposited plasmids, they undergo complete plasmid sequencing using an in-house Illumina...

32. Plasmids 101: The Wide World of Natural Plasmids

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    Blog Post

    ...host from attacks by other organisms. To conserve energy, these specialized degradative capabilities are...

33. Making CRISPR Plasmids Using Fragmid

    Type

    Blog Post

    ...Modular vector assembly enables rapid assessment of emerging CRISPR technologies. Cell Genomics, 4(3), 100519...

34. Cpf1 Update: Comparison to Cas9 and NgAgo

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    Blog Post

    ...from this publication at Addgene. 3. Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, ...

35. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type

    Blog Post

    ...common ancestry with worms before evolutionary divergence. Nikolaus Rajewsky from the Max Delbruck Center...

36. The Materials Science of Optogenetics Experiments

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    Blog Post

    ... excitation). Lasers can be purchased through Laserglow, which caters to optogenetics users and adjusts...

37. Experimenting in an Open Source lab: from CRISPR to Cats

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    Blog Post

    ...on speech recognition, he returned to school to merge his interests in biology and computing. In 2009,...

38. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type

    Blog Post

    ... spectrum flow cytometry (“spectral flow”) has emerged as a particularly useful tool for those wishing...

39. PhD Applications After COVID

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    Blog Post

    ...what comes next?” For those finishing up an undergraduate education or ending a Master’s program, the ...

40. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type

    Blog Post

    ...they have fewer hydrophilic surfaces, require detergents or lipids to remain folded, and are often less...

41. The Scientific Conference Poster Session: Tips for Success

    Type

    Blog Post

    ... your feet. Pack water and snacks to keep you energized throughout the session, bring extra clips to hold...

42. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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    Blog Post

    ...Mobile-CRISPRi from the labs of Jason Peters, Oren Rosenberg, and Carol Gross provides a streamlined suite ...

43. PCR Applications for Copy Number Variation Assays

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    Blog Post

    ...10.1007/978-1-4939-7778-9_9. PMID: 29717442. Arya M, Shergill IS, Williamson M, Gommersall L, Arya N, Patel ...

44. Viral Vectors 101: Voltage Indicators

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    Blog Post

    ... communication and function depend on potential energy. Cellular activities like action potentials, muscle...

45. Magnetic Control of Proteins: More than a Dream

    Type

    Blog Post

    ....github.io/gfp_magnetofluorescence. Karki, N., Vergish, S., & Zoltowski, B. D. (2021). Cryptochromes: ...

46. Plasmids 101: Protein Expression

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    Blog Post

    ...optimized. Yeast cells are also known for hyperglycosylating proteins which may be an issue depending ...

47. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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    Blog Post

    ...two molecules more closely is Förster Resonance Energy Transfer, FRET. Additional opportunities: Tracking...

48. Addgene's Cancer Collection Pages Connect the Research Community

    Type

    Blog Post

    ...­can directly inhibit the ability of a cell to undergo programmed cell death while simultaneously stimulating...

49. Light Sheet Fluorescence Microscopy

    Type

    Blog Post

    ... images of a sample from two opposing sides and merge the images, such as in the Ultramicroscope.  In ...

50. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    Blog Post

    ... 96-well kit - everything is so easy. We have undergrads come in for short projects and they end up making...