We narrowed to 400 results for: pho;

1. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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   ...CRISPR-Cas activity. For example, fusing AcrA4II to the photo-sensitive LOV2 domain allows anti-CRISPR activity...

2. How to Prepare for an Industry Interview

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   ...PI of the lab and for one position, I just had a phone interview. I also transitioned between different...

3. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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   ...quantification of various intermediate steps, a sizing electrophoretic gel, and qPCR quantification of the final libraries...

4. Hot Plasmids and Viral Preps - March 2021

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   ....   G-baToN can also be applied to other fluorophore/antibody pairs. Because the anti-GFP nanobody...

5. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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   ...change to large insertions like the addition of a fluorophore or tag. To utilize HDR, a donor template containing...

6. Overcoming the Challenges of Lentiviral Production

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   ...transfection method (i.e. polyethyleneimine, calcium phosphate or liposomal methods), the volume of the reaction...

7. Popular Retroviral Vectors and Their Uses in Scientific Research

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   ...genes via infection with a single ecotropic or amphotropic MuLE lentivirus provides significant genetic ...

8. Adenoviral Vector Production and Troubleshooting

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   ...looking at absorbance at 260nm using UV/Vis spectrophotometry. An absorbance of at 260nm corresponds to...

9. CRISPR in the Clinic

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   ...CRISPR spin. CRISPR CAR-T treatments for leukemia, lymphoma, and some solid tumors are currently in early-...

10. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    ... Addgene account username. Shipping address and phone number. Plasmid sequences or GenBank files. We encourage...

11. Plasmids 101: Modular Cloning

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    ...Level 1 TUs with unique promoters, and the ideal phosphatase (or any modifying enzyme) to pair with it can...

12. Hot Plasmids February 2024

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    ...Ando et al., 2023). This variant has similar photostability and brightness compared to the original, though...

13. 5 Tips for Troubleshooting Viral Transductions

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    ...fibronectin-enhanced retroviral transduction of primary human T lymphocytes for adoptive immunotherapy." Acute Leukemias ...

14. Gaining Leadership Skills Volunteering at a Professional Organization

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    ... with other people mostly online and on monthly phone calls. This has its challenges as meeting in person...

15. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

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    ...discuss important issues in person or over the telephone instead of email. As you cannot see a person’s...

16. Hot Plasmids and Viral Preps - May 2021

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    ...page.  A new system uses light to deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage...

17. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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    ... of the highly conserved glutamine-fructose-6-phosphate aminotransferase (glmS) gene in the recipient ...

18. Degrading DNA with Cascade-Cas3

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    ...Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho, E., Xu, H., Sasakawa, N., Naito, Y., Nakada, S...

19. AAV Vector Quality Control: Going the Extra Mile with NGS

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    ...’d like us to discuss. We are also available by phone or at [email protected] to answer your questions ...

20. Cloning Mammalian Cells with the Agarose Method

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    ...using cloning rings in terms of growth rates, morphology or other physiological properties. An advantage...

21. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    ...mother. Inside the pupal case, the larva will metamorphose into its adult form. In all, it takes roughly...

22. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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    ...setup for a different fluorescent signal) and photobleaching i.e. FP stability. One should also be aware...

23. Fluorescent Proteins 101: Fluorescent Protein Timers

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    ...change is usually hypothesized to be a result of chromophore oxidation. Some of the more popular timers (whether...

24. Fluorescent Tagging of Endogenous Genes with SapTrap

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    ...about Fluorescent Protein Timers Check out Photoactivatable Fluorescent Proteins Use Rosella to Monitor...

25. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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    ...form teratomas [11]. iPSCs also possess similar morphologies, growth properties, and express stem cell markers...

26. Genome-wide Screening Using CRISPR

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    ...a given phenotype. Image Credit: www.flickr.com/photos/72005145@N00/5600978712/ What CRISPR-based ...

27. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    ...non-radiative transfer for energy between neighboring fluorophores, FRET can be used to detect the inter- and intramolecular...

28. Great Results Start with Great Standard Curves

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    ... absorbance of both is then measured on a spectrophotometer and concentration versus absorbance is plotted...

29. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    ...Cas9, such as visualizing genomic loci using fluorophore tagged dCas9 may require the use of plasmid or...

30. Simplify Cloning with in vivo Assembly

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    ...unamplifiable’ Check your amplification by gel electrophoresis. In our experience, good amplification after...

31. Plasmids 101: Protein tags

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    ...tag for purification? Or you want to combine a fluorophore with a tag that localizes your protein to the...

32. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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    ...provided moral support was invaluable.  Mixing metaphors and scientists - Culturing a scientific network...

33. Save Time with Transient Plant Leaf Transformations

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    ...used in C. roseus, but processes specific to photosynthetic active tissues cannot be studied in hairy roots...

34. Designing Your Chalk Talk for the Academic Job Interview

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    ...distance runner, gardener, cook, and outdoor photographer.  Additional resources on the Addgene blog ...

35. Antibodies 101: The Four ELISAs and When to Use Them

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    ... as horseradish peroxidase (HRP) or alkaline phosphatase (AP). Fluorescent tags may also be used, but ...

36. Hot Plasmids - November 2023

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    ... promoter. For example, neurons treated with a phorbol ester activator gave fluorescent signal in the ...

37. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    ...will be cleaved resulting in activation of the fluorophore. The fluorescent signal can be used as an indicator...

38. Transferable Skills Guide: Career Planning Resources

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    ...HR, greatly increasing your chances of getting a phone interview! If you don’t have a particular employer...

39. Career Insights: Technical Support Specialist

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    ...support roles are primarily office based, and email/phone are the most popular ways to communicate with customers...

40. 5 Reasons to Use Reddit for Science Communication

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    ...notes which Evernote even allows me to access on my phone. I use it for everything from receipts to blogs ...

41. Healthcare Consulting: A Door to the Business of Life Sciences

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    ...chemotherapies, or developing innovative mobile phone applications and services that help diabetic patients...

42. Hot Plasmids Spring 2024

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    ...6).   Figure 6: Illustration of split fluorophore assay to test reconstitution via mRNA trans-splicing...

43. Plasmids 101: Broad Host Range Plasmids

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    ...Gluconacetobacter xylinus, Gluconobacter spp., Hyphomicrobium spp., Klebsiella aerogenes, K. pneumoniae, ...

44. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    ... regulated by guanosine [corrected] 3', 5'-bispyrophosphate: a model for programmed bacterial cell death...

45. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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    ...but rather because of the correct folding and fluorophore formation by most, if not all, of the FP fusions...

46. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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    ...440 or 1000bp overlaps to deliver the alkaline phosphatase gene to airway epithelial cells in mice. This...

47. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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    ...cleaves a nearby RNA sequence that contains a fluorophore that is quenched when intact and released when...

48. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    ...in several patients developing T-cell acute lymphoblastic leukemia due to insertional mutagenesis (Howe...

49. Tips for Getting a Faculty Position

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    ... my applications all by snail mail and only had phone interviews. We didn't have Skype in 2004. I also...

50. Transferable Skills Guide: Time Management

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    ...Probably the easiest way to do this is to leave your phone in your bag on “Do Not Disturb” during the times...