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  1. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...PhDs Later this week, postdocs and students will again gather in Boston - this time with the aim of finding...University, has recently published an article arguing against reducing the number of graduate students accepted...

  2. 6 Tips for Grant Writing

    Type
    Blog Post
    ...yourself a bit of a break before you read over it again with fresh eyes and correct it (and, believe me,...second) you won’t succeed, but you must try, try again. Now, this does not mean that you should apply for...

  4. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...landmark study showed significant clinical efficacy against a form of blindness known as Leber’s congenital...example, prevalence of neutralizing antibodies against some AAV types, AAV1, 2, 3 and 5 are thought to...

  5. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...microcontroller and a motor controller and made it spin again!  We’ve also learned to be resourceful and to repurpose...Illumina GAIIx we recently acquired  for sequencing again - it’s too inefficient and the reagents are too ...

  7. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...detect mEPSPs, it also enabled imaging of their propagation along dendrites for the first time (Figure 3)...Ashley Waldron We recently added two antibodies against important targets for neurodegeneration research...

  8. Of Myc and Men

    Type
    Blog Post
    ...over the years, several alternative antibodies against the Myc tag have been generated in an attempt to...al.’s results mean the end of 9E10’s time in the saga of the Myc tag? Hardly! 9E10’s accessibility and...

  9. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...Mobile-CRISPRi stably integrates into the genome and is propagated during normal DNA replication and cell division...recipient's genome. Antibiotic plates lacking DAP select against donor bacteria and for recipients with an integrated...

  11. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...negative selection marker HSV-tk is used to select against random recombinants.   Design your targeting...recombination, the negative selection marker selects against improper, random recombination into a different...

  12. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...affected by the plasmid size, nature of the insert, propagation strain, growth conditions, and the material used...Sub-culturing directly from a frozen glycerol stock or agar stab may lead to loss of the plasmid, and using ...

  13. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ... used for recombinant antibody production is Komagataella phaffii (a.k.a  Pichia pastoris) because it ... density of the antibody bands can be measured against the protein standard and the concentration may ...

  14. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ... turned to characterizing its cleavage pattern. Again, they were in for a surprise! Whereas Cas9 generates... of the PAM may give Cpf1 the ability to cleave again and possibly mediate HDR. This “second chance” mechanism...

  15. Plasmids 101: Protein tags

    Type
    Blog Post
    ...purification with glutathione, can also protect against proteolysis. That’s one form of instability. Prokaryotes...Good for purification with glutathione; protects against proteolysis, but may reduce solubililty HA YPYDVPDYA...

  16. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...Optogenetics Guide γ-Retrovirus Guide Protocols Agarose Gel Electrophoresis Bacterial Transformation How...What is a Plasmid? How to Design Primers for PCR Agarose Gel Electrophoresis Inoculating Liquid Bacterial...

  17. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...chromosomal replication and balance population growth against sustainability. Plasmid DNA replication and regulation...proteins. In nature, such plasmids ensure their propagation by limiting their copy number and compensating...

  18. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...-LV [+M], negative control) were immunoblotted against Anti-SARS-CoV-2 Nucleocapsid Protein antibodies...middle panel). Samples were also immunoblotted against vinculin as a loading control (right panel). Image...

  19. Viral Production at Addgene

    Type
    Blog Post
    ...possible, all plasmids used for viral production are propagated in the endA-mutated NEB Stable strain of E. coli...plasmid in the viral preparation by performing PCR against unique regions of the transfer plasmid.   Lentivirus...

  20. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ... code, we may need additional clarification and again we collaborate with the Product Team who in turn...would be like re-inventing the wheel over and over again for every single software today that prints labels...
Showing: 121 - 140 of 393 results