...different packaging capacities depending on the virus. AAV has a packaging capacity of ~4.7 kb, while lentivirus... lentivirus has a capacity of 8–10 kb. Time Viral production in mammalian cells is relatively quick. After...transfer plasmids have a much larger packaging capacity — up to 38 kb! Generally, insect cell production...

...genes; and the pRepCap plasmid containing replication factors and serotype-specific capsid genes.  In this...genome-containing (full) and non-genome-containing (empty) capsids, thus leading to an overestimate of transducible...1999). Titration of AAV-2 particles via a novel capsid ELISA: Packaging of genomes can limit production...

...FP-fusion to rhodopsin resulted in electrochromic FRET capable GEVIs, with rhodopsin quenching the added fluor...available with red-shifted fluorophores (find FRET capable GEVIs here!). The only issue with these GEVIs? ...sensing component and a self-labeling domain that is capable of binding a Janelia Fluor (JF). The voltage-dependent...pseudotyping is also a powerful tool that swaps capsid proteins from different viruses to optimize them...

...but only transiently. However new viral vectors capable of providing long-term gene expression in retinal...and severe visual impairment that faithfully recapitulated the human condition. Luck brought all these ...integration into the genome, and its large DNA packaging capacity is well suited for larger transgenes. Unfortunately...further improve the technology: engineering of new capsid variants, designing expression cassettes to regulate...

...affinity purification in situ of regulatory elements (CAPTURE) (Liu et al., 2017). This system utilizes dCas9...through proteomics and the chromosome conformation capture (3C) technique. In addition to 3xFLAG and biotin...when inappropriately expressed can cause facioscapulohumeral muscular dystrophy (FSHD), a disease for ...Central PMCID: PMC6286148. Liu, Xin, et al. "In situ capture of chromatin interactions by biotinylated dCas9...

...binding capacity and high protein retention. It comes in a variety of pore sizes and protein capacity, allowing...are not with denaturing agents like DTT or β-mercaptoethanol.  Denaturing proteins To denature your proteins...you'll want to use SDS and either DTT or β-mercaptoethanol (BME). DTT is a stronger reducing agent than... is a little quicker to use, with high binding capacity, though it has lower retention after binding and...

...look specifically at the characteristics and capabilities an ELN needs to facilitate true reproducibility...without access to them, the record of research captured in the ELN is incomplete. Thus, to support reproducibility...for discussion and review. It’s also possible to capture Slack conversations and import them into RSpace...and workflows that went into it. The ability to capture the conversations and associate them with a particular...

.... If you missed it, here’s the recap! The twelve days of CRISPR recap On the first day of CRISPR, Addgene...day of CRISPR, Addgene gave to me: a method to capture promoter-enhancer 3D chromatin structures using...

...Transfer Plasmid : 1 pHelper: 1 RepCap OR 3 Transfer Plasmid : 5 pHelper: 2 RepCap Select what type of tissue...using 28 μg of total plasmid (Transfer, helper, and RepCap added together) per 15 cm dish or 2500 μg of plasmids...

...addition, P. pastoris has the capacity to glycosylate proteins and may recapitulate the post-translational modifications...yeast Pichia pastoris. The improved secretory capacities of these microbes make them useful for producing...

... chaotropic salts. Capillary electrophoresis Quantifying DNA using capillary electrophoresis is similar...Figure 1: The quantification of a RNA ladder using capillary electrophoresis which shows fluorescence units...

... can rupture E. coli spheroplasts to release encapsulated proteins. Video by ACS. For a more cartoony ...Harvard Medical School. Follow her on Twitter @jessicapolka.     References 1. Kanabrocki, J. A., Quackenbush...Protein Piston That Breaks Membranes to Release Encapsulated Cargo. ACS Synth. Biol. (2016). doi:10.1021/...

... will be necessary to improve its multiplexing capacity. Multiplexing is key due to the natural redundancy...multiplexing in systems with limited packaging capacity. Still confused about the various tRNA expression...References Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system. Xie...

... by intravenous injection of PHP.eB, an AAV capsid capable of broad transduction of the nervous system...the Addgene blog Read more about the PHP.eB AAV capsid Read more about optogenetics Learn about the differences...

... below:   ID Cv (Capsid) FLAG Tag Terminus 79629 (N) and 79628 (C) Cv (capsid) N and C 79633 (N...system has a much larger cargo capacity than lentivirus and is capable of efficiently creating stable ...homology (usually 30-40bp overlaps). To expand the capabilities of Gibson assembly and make a more modular platform...

...Recent developments promise to further change the landscape facing all scientists, both young and established...potential to profoundly shift the academic science landscape: the treatment of trainee scientists as workers...shifts caused to the practical and philosophical landscape of the scientific enterprise will provide challenges...

...counterparts, so they will be easier to deliver in low-capacity vectors like AAV. AAV (adeno-associated viral ...tissues. However, these vectors have a low genetic capacity of ~4.5 kb. At 4.1 kb, SpCas9 barely fits into...understand where Cpf1 fits into the genome editing landscape. References 1. Kim D, Kim J, Hur JK, Been KW...

...biology of the worm into a computer model that recapitulates how molecular biology impacts worm locomotion...mutant worm. Like many academic labs, we have the capability to study many different mutants because the Caenorhabditis...and cobble together quite a diverse array of capabilities. So don’t throw old equipment away until you...

... to the establishment of in-house sequencing capabilities and Seqwell’s plexWell technology, we can now...small predetermined seed sequences unique to the capsid plasmid. Our recombination software looks for and... Moullier P, Salvetti A (2005) Evidence for Encapsidation of Prokaryotic Sequences during Recombinant ...

... E1 region). The space in the viral particle’s capacity that is made available by this deletion from the... (and their structural proteins, which would encapsidate new virions) are not expressed. Note that replication-competent...genome, and adenoviral vectors all have a genomic capacity that is much higher than other viral vectors (...