We narrowed to 184 results for: CO
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TypeBlog Post...was contributed by Nikolai Braun and Keira Havens, co-founders of Revolution Bioengineering. Last year...
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Brain Armamentarium
TypeCollection...combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors. Jonathan... -
Viral Vectors 101: Chemogenetics
TypeBlog Post...also activate muscarinic-derived DREADDs. A ligand co-developed by Bryan Roth's and Takafumi Minamimoto's... -
Adenoviral Vector Production and Troubleshooting
TypeBlog Post... the ITRs and packaging signal in cis. Therefore, co-infection with a helper adenovirus is required to... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post... the final targeting vector (Fig. 1, Step 4), and co-injection of the targeting vector and a Cas9 expression... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog Post... under-expressing clones. If GFP-encoding cDNA is co-transfected at a favorable ratio to the gene of interest... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog Post... with an ssDNA guide. Therefore, researchers must co-transfect cells with 5’-P-ssDNA guides and an NgAgo... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog Post...chromophore forms spontaneously and without additional co-factors, substrates, or enzymatic activity – it only... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog Post...explains how lentiviral particles are produced by co-transfecting three essential ingredients: the lentiviral... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog Post...data and computing inside living cells. He's also a co-founder of the BioBricks Foundation, an organization... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog Post...barriers to implementation. Funded by the NSF (with Co-PIs Jessica Bell, Lisa Gentile, Ellis Bell, and Amy... -
Antibodies 101: Immunoprecipitation
TypeBlog Post...linkage. This means that the primary antibody will not co-elute with the target protein, eliminating the risk... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog Post... own nuclease activity. Instead, it forms a novel co-complex with the transposition protein TniQ for targeting... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post... BiFC constructs. If your PIPs actually interact, co-expressing one of the endogenous PIPs should decrease... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post...out two Bdnf isoforms (I and IV) for investigation. Co-transduction of primary rat neurons with dCas9-VPR... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog Post...intimidating, but I had my lab’s protocols and some great co-workers to help me out. Even with these tools, I ... -
Simplify Cloning with in vivo Assembly
TypeBlog Post...fragment, encode homology arms to allow immediate co-transformation with linearized vector backbone. ... -
AAVs in Retinal Gene Therapy
TypeBlog Post...a result, Luk Vandenberghe and colleagues at MEEI co-founded a non-profit to help push forward candidate... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog Post...expressing the inducer (i.e. tet-off transactivator) is co-injected. The dose of this AAV can be adjusted to... -
Lentiviral Vector Uses and Overview
TypeBlog Post...viral packaging and host genome integration. After co-transfection of these plasmids, 293T cells released...