We narrowed to 191 results for: CO
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TypeBlog Post...three Nobel Prize winners, one NIH Director and a co-founder of BGI-China presenting, it’s no wonder it...
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Genome-wide Screening Using CRISPR
TypeBlog Post...’ to a protospacer adjacent motif (PAM) sequence. Co-delivery of wild-type Cas9 and a gRNA generates a... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog Post...was contributed by Nikolai Braun and Keira Havens, co-founders of Revolution Bioengineering. Last year... -
Brain Armamentarium
TypeCollection...combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors. Jonathan... -
Lentivirus Plasmids
TypeCollection... EF-1alpha driven expression of cDNA and and EGFP co-expression. See plasmid 21374 for dtomato version... -
Viral Vectors 101: Chemogenetics
TypeBlog Post...also activate muscarinic-derived DREADDs. A ligand co-developed by Bryan Roth's and Takafumi Minamimoto's... -
Adenoviral Vector Production and Troubleshooting
TypeBlog Post... the ITRs and packaging signal in cis. Therefore, co-infection with a helper adenovirus is required to... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog Post... with an ssDNA guide. Therefore, researchers must co-transfect cells with 5’-P-ssDNA guides and an NgAgo... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post... the final targeting vector (Fig. 1, Step 4), and co-injection of the targeting vector and a Cas9 expression... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog Post... under-expressing clones. If GFP-encoding cDNA is co-transfected at a favorable ratio to the gene of interest... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog Post...chromophore forms spontaneously and without additional co-factors, substrates, or enzymatic activity – it only... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog Post...explains how lentiviral particles are produced by co-transfecting three essential ingredients: the lentiviral... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog Post...data and computing inside living cells. He's also a co-founder of the BioBricks Foundation, an organization... -
CRISPR Guide
TypeCollection... 8E). In a 2-vector system, you’ll need to either co-infect with a Cas9-producing lentivirus or use a ...genes in mammalian cells include: SunTag system - co-expression of epitope-tagged dCas9 and antibody-activator...two-component system (Figure 9C) SAM activators - co-expression of dCas9-VP64 with a modified scaffold...non-repetitive genomic locus can also be labeled by co-delivering multiple gRNAs that tile the locus. Chromosome...dCas9 by fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin ligase. Whatever the chosen...other Cas enzymes, the Cascade-Cas3 system has been co-opted for use in eukaryotic cells . The Cascade complex... -
Antibodies 101: Immunoprecipitation
TypeBlog Post...linkage. This means that the primary antibody will not co-elute with the target protein, eliminating the risk... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog Post...barriers to implementation. Funded by the NSF (with Co-PIs Jessica Bell, Lisa Gentile, Ellis Bell, and Amy... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post... BiFC constructs. If your PIPs actually interact, co-expressing one of the endogenous PIPs should decrease... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post...out two Bdnf isoforms (I and IV) for investigation. Co-transduction of primary rat neurons with dCas9-VPR... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog Post...intimidating, but I had my lab’s protocols and some great co-workers to help me out. Even with these tools, I ... -
Simplify Cloning with in vivo Assembly
TypeBlog Post...fragment, encode homology arms to allow immediate co-transformation with linearized vector backbone. ...