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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 188 results for: Coli

Showing: 121 - 140 of 188 results

  1. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...specificity and binding affinity, can be produced in E. coli, and are both soluble and easily engineered.  Monobodies...

  3. CRISPR in the Clinic

    Type
    Blog Post
    ...infections. The Cas3 enzymes target several strains of E. coli and lethally chop their genomes into many, many ...

  7. Pooled Library Amplifications

    Type
    Blog Post
    ...transformation amplification protocol is the Chong Zhang E. coli Genome-wide Inhibition Library. DNA and cell amounts...

  10. Hot Plasmids - August 2020

    Type
    Blog Post
    ...Nanobodies are easily expressed in the cytosol of E. coli, however the nanobody proteins tend to get stuck...inclusion body problem and makes nanobody production in E.coli as simple as following a DNA miniprep protocol!... were expressed in SHuffle T7 Express competent E.coli (NEB) using IPTG induction. To biotinylate the ...

  12. Viral Production at Addgene

    Type
    Blog Post
    ...propagated in the endA-mutated NEB Stable strain of E. coli. In addition, plasmids are typically prepared using...

  14. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...fluorescence-activated cell sorting (FACS) of E. coli transformed with aptamer libraries, the Jaffrey ...nucleotide aptamer they called Broccoli. As a product of SELEX and FACS, Broccoli is inherently more stable...new RNA-based scaffolds to contain and stabilize Broccoli cassettes, thereby extending their use into mammalian...mammalian cells. They’ve also dimerized Broccoli to further enhance its biosensor capabilities (see Fig...et al. “In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies....Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene View All Fluorescent Protein...

  17. CRISPR Antimicrobials

    Type
    Blog Post
    ...beta-lactam and quinolone resistance genes in E. coli using plasmid and phagemid delivery systems. Phagemids...
Showing: 121 - 140 of 188 results