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We narrowed to 186 results for: Coli

Showing: 121 - 140 of 186 results

  1. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...specificity and binding affinity, can be produced in E. coli, and are both soluble and easily engineered.  Monobodies...

  3. CRISPR in the Clinic

    Type
    Blog Post
    ...infections. The Cas3 enzymes target several strains of E. coli and lethally chop their genomes into many, many ...

  5. Molecular Biology Reference

    Type
    Guide
    ...to use in an experiment. E. coli strains for propagating plasmids E. coli are gram-negative, rod shaped...Addgene blog posts relating to common E. coli lab strains and E. coli strains specialized for protein expression... with Plasmids Introduction Types of Plasmids E. coli strains for propagating plasmids Antibiotics commonly...For instance, most plasmids are replicated in E. coli and are relatively small (∼3000 - 6000 basepairs.... When a plasmid exists extrachromosomally in E. coli , it is replicated independently and segregated ...many different naturally occurring strains of E. coli , some of which are deadly to humans. The majority...majority of all common, commercial lab strains of E. coli used today are descended from two individual isolates...

  8. Pooled Library Amplifications

    Type
    Blog Post
    ...transformation amplification protocol is the Chong Zhang E. coli Genome-wide Inhibition Library. DNA and cell amounts...

  13. Hot Plasmids - August 2020

    Type
    Blog Post
    ...Nanobodies are easily expressed in the cytosol of E. coli, however the nanobody proteins tend to get stuck...inclusion body problem and makes nanobody production in E.coli as simple as following a DNA miniprep protocol!... were expressed in SHuffle T7 Express competent E.coli (NEB) using IPTG induction. To biotinylate the ...

  14. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...fluorescence-activated cell sorting (FACS) of E. coli transformed with aptamer libraries, the Jaffrey ...nucleotide aptamer they called Broccoli. As a product of SELEX and FACS, Broccoli is inherently more stable...new RNA-based scaffolds to contain and stabilize Broccoli cassettes, thereby extending their use into mammalian...mammalian cells. They’ve also dimerized Broccoli to further enhance its biosensor capabilities (see Fig...et al. “In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies....Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene View All Fluorescent Protein...

  16. Viral Production at Addgene

    Type
    Blog Post
    ...propagated in the endA-mutated NEB Stable strain of E. coli. In addition, plasmids are typically prepared using...

  18. CRISPR Antimicrobials

    Type
    Blog Post
    ...beta-lactam and quinolone resistance genes in E. coli using plasmid and phagemid delivery systems. Phagemids...
Showing: 121 - 140 of 186 results