... of glucose dehydrogenase and generated a strain that could achieve glucose dehydrogenase expression that... crRNA can be customized as well, including concatenations into crRNA arrays targeting multiple different...

...generous donation of a Illumina MiSeq sequencer enabled us to perform the necessary sequencing on-site,...how the various pieces were connected, as this enabled us to rapidly iterate and optimize our pipeline...used Nextflow Tower to augment our pipeline and enable our team members to automate their weekly sequencing...

...eukaryotic genome to be entirely sequenced and is very amenable to genetic manipulation. Yeast cells are great...molecular biology processes. C. elegans are also quite amenable to genetic manipulation. Transgenic animals can...gene transfer in C. elegans: extrachromosomal maintenance and integration of transforming sequences." The...

...plant-specific changes to CRISPR components are necessary to enable genome editing in plant cells.   This blog post... empty gRNA backbones also contain Cas9, which enables expression of both Cas9 and the gRNA off of the... nanomaterials with chemical cargoes in plants enabled by a biorecognition motif. Nat Commun 11: . https...

...CRISPR has revolutionized molecular biology and has enabled functional studies in unprecedented ways. With ...This method is facilitated by DNA constructs that enable insertion of ~400 bp complementary to your gene...between targets) or multiple target sequences are concatenated. hpRNA has been extensively used in plant research...

...non-mammalian systems, but it is not confined to that arena! The Mammalian Toolkit was engineered specifically... Assembly Standard and New E. Coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional...McGee, Abby V., et al. Modular Vector Assembly Enables Rapid Assessment of Emerging CRISPR Technologies...

...Scores of FP variants have been created, thereby enabling a broad spectrum of donor fluorescence wavelengths...importantly, these compounds cross cell membranes, thus enabling FRET measurements in intact cells. The Gradia ...similarly but with differing R0 values, thereby enabling calibrated FRET distance measurements (for an ...

...keratinocytes from hair pluck, peripheral blood cells, and renal epithelial cells from urine are some of the easily...β-like-cells with the expression of pancreatic and duodenal homoeobox gene1 (PDX1) [40]. In recent works, ...3690668. 40. Ferber, S., et al., Pancreatic and duodenal homeobox gene 1 induces expression of insulin ...

...a thorough yet concise rundown of how recently enacted labor laws will likely increase postdoc earnings...graduate school classmates, Ben Vincent and Niroshi Senaratne. When we recorded, Ben and Niroshi, were finishing...

...senior graduate students, Ben Vincent and Niroshi Senaratne, from the Biological and Biomedical Sciences program...Biology, and he tweets @benjvincent.     Niroshi Senaratne is a graduate of the PhD program in Genetics and...

...about how FRET can be used to study biological phenomena and about how fluorophores other than fluorescent...Expression in Mammals Recombinase-based State Machines Enable Order-dependent Logic in vivo As always, feel ...

...series, we continue our conversation with Niroshi Senaratne and Ben Vincent from the Biological and Biomedical...Biology, and he tweets @benjvincent.     Niroshi Senaratne is a graduate of the PhD program in Genetics and...

...researchers worldwide. Countless breakthroughs enabled by shared resources. As we step into 2025, these...every collaboration formed, and every breakthrough enabled brings us closer to making an impact on humanity's...

...collaboration, members of Maureen Hanson’s and Rima Menassa's labs made the accidental discovery that laser ...References Sattarzadeh A, Saberianfar R, Zipfel WR, Menassa R, Hanson MR. Green to red photoconversion of GFP...

...restriction enzymes for cloning (see schematic below), enabling a researcher to test multiple tags for one protein...pCri constructs: pCri-1a, -4a and -9a. 6xHis tags enable affinity purification using a Ni-NTA resin. MBP...

...promote the awards. References 1. Martinez-Klimova, Elena et al. “Endophytes as sources of antibiotic” Biochemical... Listen to Our Interview with Previous Awardee Kwabena Duedu Resources at Addgene.org Check out our ...

...exhibit fast kinetics (10 ms on and 100 ms off), enabling detection of individual peaks of DA release.  ... al. "A genetically encoded fluorescent sensor enables rapid and specific detection of dopamine in flies...

...the AAV9-X1.1 serotype.  In rodents, AAV9-X1.1 enables expression in brain endothelial cells via an injection...They work via newly-characterized enhancers that enable a whole host of (awesome) research not easily carried...

...usually llamas or alpacas). But because sdAbs are so amenable to recombinant expression (and because maintaining...family of tools, the applications they have already enabled, and the discoveries yet to come.      References...

...are critical tools for many fields of biology and enable the conversion of RNA templates to DNA but suffer...protein binding at the TERT gene, but this method enables the unbiased discovery of proteins involved in ...