Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 443 results for: ENA

Showing: 121 - 140 of 443 results

  1. Bioinformatics at Addgene

    Type
    Blog Post
    ...generous donation of a Illumina MiSeq sequencer enabled us to perform the necessary sequencing on-site,...how the various pieces were connected, as this enabled us to rapidly iterate and optimize our pipeline...used Nextflow Tower to augment our pipeline and enable our team members to automate their weekly sequencing...

  2. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...standard, denaturing Western blot will not be suitable for an IP, as proteins in an IP are not denatured (learn...for an IP, samples are typically lysed in a non-denaturing buffer containing non-ionic detergents, such ...

  3. Hot Plasmids - November 2023

    Type
    Blog Post
    ...that overcome some current prime limitations and enable impressive editing efficiencies in vitro and in...simplifying the production process and could even enable the creation of CAR-T cells directly within the...Engineered allostery in light-regulated LOV-Turbo enables precise spatiotemporal control of proximity labeling...

  4. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ... of glucose dehydrogenase and generated a strain that could achieve glucose dehydrogenase expression that... crRNA can be customized as well, including concatenations into crRNA arrays targeting multiple different...

  5. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...implemented and from that, develop better software.  Maintenance  We’re not just about developing new features...: we take time during each sprint to do some maintenance work on our code. One way to look at this is ...continuing to add new features.  Another area of maintenance is agreeing to the names of the things in our...

  6. Five Popular Model Organisms

    Type
    Blog Post
    ...eukaryotic genome to be entirely sequenced and is very amenable to genetic manipulation. Yeast cells are great...molecular biology processes. C. elegans are also quite amenable to genetic manipulation. Transgenic animals can...gene transfer in C. elegans: extrachromosomal maintenance and integration of transforming sequences." The...

  7. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...plant-specific changes to CRISPR components are necessary to enable genome editing in plant cells.   This blog post... empty gRNA backbones also contain Cas9, which enables expression of both Cas9 and the gRNA off of the... nanomaterials with chemical cargoes in plants enabled by a biorecognition motif. Nat Commun 11: . https...

  8. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...CRISPR has revolutionized molecular biology and has enabled functional studies in unprecedented ways. With ...This method is facilitated by DNA constructs that enable insertion of ~400 bp complementary to your gene...between targets) or multiple target sequences are concatenated. hpRNA has been extensively used in plant research...

  9. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...non-mammalian systems, but it is not confined to that arena! The Mammalian Toolkit was engineered specifically... Assembly Standard and New E. Coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional...McGee, Abby V., et al. Modular Vector Assembly Enables Rapid Assessment of Emerging CRISPR Technologies...

  10. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...Scores of FP variants have been created, thereby enabling a broad spectrum of donor fluorescence wavelengths...importantly, these compounds cross cell membranes, thus enabling FRET measurements in intact cells. The Gradia ...similarly but with differing R0 values, thereby enabling calibrated FRET distance measurements (for an ...

  12. Editor's Choice, October 2016

    Type
    Blog Post
    ...a thorough yet concise rundown of how recently enacted labor laws will likely increase postdoc earnings...graduate school classmates, Ben Vincent and Niroshi Senaratne. When we recorded, Ben and Niroshi, were finishing...

  13. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    ...senior graduate students, Ben Vincent and Niroshi Senaratne, from the Biological and Biomedical Sciences program...Biology, and he tweets @benjvincent.     Niroshi Senaratne is a graduate of the PhD program in Genetics and...

  14. Editor's Choice, July 2016

    Type
    Blog Post
    ...about how FRET can be used to study biological phenomena and about how fluorophores other than fluorescent...Expression in Mammals Recombinase-based State Machines Enable Order-dependent Logic in vivo As always, feel ...

  15. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ...series, we continue our conversation with Niroshi Senaratne and Ben Vincent from the Biological and Biomedical...Biology, and he tweets @benjvincent.     Niroshi Senaratne is a graduate of the PhD program in Genetics and...

  16. Reflections and Looking Ahead

    Type
    Blog Post
    ...researchers worldwide. Countless breakthroughs enabled by shared resources. As we step into 2025, these...every collaboration formed, and every breakthrough enabled brings us closer to making an impact on humanity's...

  17. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...collaboration, members of Maureen Hanson’s and Rima Menassa's labs made the accidental discovery that laser ...References Sattarzadeh A, Saberianfar R, Zipfel WR, Menassa R, Hanson MR. Green to red photoconversion of GFP...

  18. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...restriction enzymes for cloning (see schematic below), enabling a researcher to test multiple tags for one protein...pCri constructs: pCri-1a, -4a and -9a. 6xHis tags enable affinity purification using a Ni-NTA resin. MBP...

  20. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...exhibit fast kinetics (10 ms on and 100 ms off), enabling detection of individual peaks of DA release.  ... al. "A genetically encoded fluorescent sensor enables rapid and specific detection of dopamine in flies...
Showing: 121 - 140 of 443 results