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We narrowed to 472 results for: FLU;

Showing: 121 - 140 of 472 results

  1. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...work!   Fluorophores When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein...us that, although fluorescent proteins are incredibly useful, chemical fluorophores can still be used ...Better Dyeing Through Chemistry & Small Molecule Fluorophores In this post, Luke Lavis from Janelia Research...variety of experiments. Read more to find chemical fluorophores for your next experiment. Nucleic acid based...to different pH’s and some that are fused to fluorescent proteins. She also showed that R-bodies can rupture...to achieve more efficient genome editing - all influenced by an understanding of Cas9 biophysics! Casilio...genes in a single cell, and localization of a fluorescent signal to your target gene. Read this post to...

  2. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...peak fluorescence and resting fluorescence (achieved through lowering sensor resting fluorescence, while...scientists access to a wide array of promoters and fluorophores for validating and testing purposes. We will...approximately twice as bright, relative to baseline fluorescence, as the GCaMP7f variant, and is approximately...variant. jGCaMP7b exhibits the brightest resting fluorescence and can be used for imaging small neuronal processes...while maintaining high peak fluorescence) and is useful for imaging signaling activity in large populations...of densely-labeled neurons because background fluorescence from inactive neurons is reduced. This variant...localization should improve the specificity of influence measurements by reducing photostimulation of the...

  3. Zebrafish Plasmid Collection

    Type
    Collection
    ...plasmids for genome editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression...markers, somatic mutations, or labeling with fluorescent proteins to visualize and track clones of cells... Albert Pan and Alex Schier Labs. Multicolor fluorescent labeling in zebrafish Whole organism lineage ...to study lipid dynamics in vivo. Bimolecular fluorescence complementation (BiFC) tools - Marco Morsch ...Michel Bagnat lab. Targeting cassettes encoding fluorescent proteins for tagging genes at endogenous genomic...consisting of plasmids with four promoters, six fluorophores, and empty vectors designed to streamline the...zebrafish for studying neurodegenerative disorders. Fluorescent Protein Fusion Construct Set - Amro Hamdoun Lab...

  4. Control AAV Preps

    Type
    Collection
    ...Constitutive 5, 8 Wilson 105532 pAAV.CMV.ffLuciferase.SV40 CMV ffLuciferase Constitutive 8 Wilson 105536 ...Browse our control AAV collection, including fluorescent proteins, spaghetti monster, and more. Viral...Control AAV Browse our control AAV that express fluorescent proteins under general promoters. These AAV can...EF1a/nEF GFAP and variants Synapsin TBG Other Fluorophore/Tag Green Red None Other Cre-dependent color ...non-cre-dependent) Clear Filters ID Name Promoter Fluorophore/Tag Activity Serotype PI 37825 AAV-CAG-GFP CAG...pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 CAG GFPsm_myc (does not fluoresce) Cre dependent 1 Looger 98928 pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40...pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 CAG Ruby2sm-Flag (does not fluoresce) Cre dependent 1 Looger 45185 AAV-EF1a-BbTagBY...

  5. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...engineer a wide variety of GFPs with diversity in fluorescence brightness, intensity, excitation and emission...derived from the Timer protein - a mutant of the fluorescent protein dsRed that changes irreversibly its color... and in vivo. Merged green and red channel fluorescence images of C2C12 cells stably expressing pTRE-Tight-MitoTimer...for 2 hours, and imaged on the Keyence BZ-9000 fluorescence microscope 8 hours post-pulse. The distinct ...distinct differences of red-to-green fluorescence in individual mitochondria in the networks marks the differences...overexpression or knock-down, where signaling dynamics are influenced by the relatively long life-cycle of a protein...expression causes the induction of both DsRed fluorescence and GFP shRNA. Mice carrying the R26DsRedR; ...

  6. Brain Armamentarium

    Type
    Collection
    ... (Alias: CN4496) Expression of SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in... (Alias: CN2610) Expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny... (Alias: CN2408) Expression of SYFP2 (yellow fluorescent protein) in striatal and neocortical Sst-Chodl... (Alias: CN2787) Expression of SYFP2 (yellow fluorescent protein) in striatal Pvalb+ neurons. Note strong... (Alias: CN3038) Expression of SYFP2 (yellow fluorescent protein) in striatal and lateral septum cholinergic...BGHpA (Alias: CN2700) Expression of mTFP1 (cyan fluorescent protein) in striatal indirect pathway medium ...BGHpA (Alias: CN2237) Expression of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium ...

  7. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...Stiles MA (2022) Improved fluorescent signal in expansion microscopy using fluorescent Fab fragment secondary...101: Introduction to Immunofluorescence  Antibodies 101: Multiplex Immunofluorescence Antibodies 101: Affinity...workings of the synapse by conventional light or fluorescence microscopy (Dani, et al., 2010). How do you ...the resolution barrier while still utilizing fluorescence microscopy, is another option. However, most...understand the ultrastructural relationships that influence everything from cell division to memory, but don...antibodies, chemical dyes, and genetically encoded fluorescent proteins (Gambarotto, et al. 2019; Tillberg, ... of cells and tissues labeled using standard fluorescent proteins and antibodies. Nat Biotechnol 34:987...

  8. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...agglutinin WGA, Schwab et al., 1978) or fluorescent molecules such as Fluoro-Gold (Schmued and Fallon, 1986) ...Schmued L.C., and Fallon J.H. (1986). Fluoro-Gold: a new fluorescent retrograde axonal tracer with numerous...either produce a colorimetric product or are fluorescent. While dyes usually spread unidirectionally, ...biotinylated dextran amines (Veenman et al., 1992) or fluorescent molecules like rhodamine-isothiocyanate (RITC...high and neuron-specific transgene expression. Fluorescent proteins are widely used for tracing and their...with an AAV9 vector expressing a Cre-dependent fluorescent marker. Injection into a conditional mutant mouse...., 2017) that express one of a few different fluorescent proteins (red, green, blue). If a researcher ...

  9. Evolution of Lab Techniques

    Type
    Blog Post
    ...labeled probes or by the presence of fluorescence for fluorescently labeled probes. Colorimetric and luminescence...nucleotides were replaced with fluorescently labeled ones allowing for fluorometric-based detection. This increased...synthesized by electronically recording fluorescence activity as fluorescently labeled nucleotides are incorporated...through hybridization of a radioactively or fluorescently labeled probe. If the DNA is complementary to...quantitative PCR as an alternative to Southern blot or fluorescence in situ hybridization for detection of gene ...DNA by restriction endonuclease of Hemophilus influenzae." Proceedings of the National Academy of Sciences...

  10. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ...elegans even easier - now, one can generate a fluorescent protein fusion, transcriptional reporter, and...self-excising cassette (SEC). To make it possible fluorescently label proteins in C. elegans, they inserted ...inserted the SEC into a synthetic intron between a fluorescent protein (FP) and a 3xFLAG tag (see construct schematic...located in this synthetic intron, and only the fluorescent protein and 3xFLAG-tag sequences are inserted...serve as the repair template plasmid to create fluorescent and 3xFLAG-tag fusion proteins. The repair template...the Roller phenotype. These animals contain a fluorescent protein fusion for the targeted gene. After initial...Dickinson et al. (2015) illustrating the steps to fluorescently tag a C. elegans protein. The his-72 locus is...

  11. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...al., 2015). To do so, they fused a different fluorescent protein to various Cas9 variants, SpCas9, NmCas9...PP7, or boxB) that each recruits a different fluorescent protein (BFP, GFP, or RFP) to the genomic loci...to the small molecule, DFHBI-1T to induce its fluorescence. They found that aptamer insertion in the gRNA...8XMS2 which will recruit the HaloTag with red fluorescent ligands to the loci of interest. Dual color detection...sgRNA-Sirius-4X(MS2-PP7)) that result in yellow fluorescence at the loci of interest, the team could visualize...4X(MS2-PP7) showing recruitment of different fluorescent proteins. (B) CRISPR-Sirius used to visualize...loci, what makes CRISPR-Sirius unique? Unlike fluorescent in situ hybridization, CRISPR-based visualization...

  12. A Control for All Seasons

    Type
    Blog Post
    ...Burry 2011). Autofluorescence Endogenous fluorescence of the sample, or autofluorescence, also interferes...without any antibodies to determine the level of autofluorescence in the sample. D, when using multiple primary...than the background noise (Waters 2009). If the fluorescence from your target is extremely bright, then the... the noise likely will not interfere. If the fluorescence from your target is weak, then it is best to...and labels omitted. Any signal remaining is autofluorescence and should be considered in the interpretation...controls are single stained samples for each fluorophore and are critical to analyze multiplex flow cytometry...Jennifer C. “Accuracy and Precision in Quantitative Fluorescence Microscopy.” Journal of Cell Biology, vol. 185...

  13. Microbiology Resources

    Type
    Collection
    ...bacterial expression system. Fluorescent Protein Guide : Addgene's extensive fluorescent protein plasmid collection...Kits for Microbiology : Sets of plasmids for fluorescence, cloning and expression, and synthetic biology... Enterococcus sp. Geobacillus sp. Haemophilus influenzae Helicobacter pylori Listeria monocytogenes Mycobacterium...Plasmids for Viruses Species Dengue Hepatitis Influenza Mumps virus Rubeola (measles) West Nile virus ...systems) and therefore supplied as one item. Fluorescent Proteins FRET-based biosensors : E. coli expression...expression - Pertz Lab Bacterial Fluorescence Labeling : Broad-Range Rainbow Vectors - Barbier Lab Cloning...

  14. Antibodies 101: Conjugation

    Type
    Blog Post
    ...together. Common conjugates in the lab Fluorophores Fluorophores allow you to detect your antibodies using...techniques like fluorescence microscopy, flow cytometry, or spectrophotometry. The range of fluorophores available...tool for comparing different fluorophores. We also touched on fluorophores as antibody conjugates in an...sure to check the spectral properties of the fluorophores you are considering to make sure that they’ll...equally, if not more, common conjugate class than fluorophores are enzymes. The most common enzyme conjugates...

  15. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    ... for awards to early career scientists using fluorescent proteins in their research. These awards help...roles in the development and dissemination of fluorescent protein tools. This year, we’ve made some changes...-generation intensiometric glutamate sensing fluorescent reporter (iGluSnFR3) to study glutamate, a signaling...disease,” says Aggarwal. This biosensor changes fluorescence based on the release of glutamate from neurons...to the roGFP2 cysteine thiols and change its fluorescence properties. The biosensor can also be used along... GCaMP7, measuring calcium signaling related fluorescence during morphine administration and withdrawal...agonist inhibits the neuron (via hM4D). She fused fluorescent protein tags to the receptors to monitor their...

  16. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ... the active site. Dronpa also produces green fluorescence when exposed to 500 nm light.  Under 400 nm ... kinase translocation reporter with a mRuby2 fluorescent reporter (ERK KTR-mRuby2). The ERK KTR is a ...active, it phosphorylates the ERK KTR and red fluorescence localizes to the cytoplasm. When MEK1 and ERK...inactive, the ERK KTR isn’t phosphorylated and red fluorescence can be found in the nucleus. For testing kinase...Addgene Blog Learn More about Photoactivatable Fluorescent Proteins Control Protein Expression with Cre-lox... KTR reporter plasmids Find photoactivatable fluorescent proteins in empty backbones in the Michael Davidson...Photoswitchable Fluorescent Proteins ...

  17. Plant Plasmids and Resources

    Type
    Collection
    ...Return to top Fluorescent Proteins The table below highlights plasmids that feature fluorescent or luminescent... You may also like... Plant CRISPR resources Fluorescent Protein Guide Addgene Blog: Plant Biology CRISPR...Cloning Transcription Factors RNAi Empty Backbones Fluorescent Proteins Other Resources Background Addgene’s...from genome engineering and gene regulation to fluorescent proteins. Addgene’s collection includes plasmids...Marillonnet and Johannes Stuttmann. Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly...you are looking for, such as the promoter or fluorescent protein of choice. ID Plasmid Description Promoter...

  18. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...and/or fusing your gene target sequence to a fluorescent reporter sequence (discussed below). Examples...Non-destructive methods, most popularly with a fluorescent reporter, directly couple silencing of your gene... that contains a target sequence against the fluorescent reporter in an inverted repeat orientation. Therefore...have readily observable phenotypes quantified. Fluorescent reporter-based RNAi constructs have been designed...presence of the RNAi transgene, followed by a fluorescence readout of RNAi activity. As a plant researcher...subpopulation can be easily achieved through fluorescence-activated cell sorting (FACS). This is not possible...visually identified as undergoing RNAi through the fluorescent reporter and manually extracted. This process...

  19. Imaging Tools and Following a Passion for Basic Biology: Interview with Joachim Goεdhart

    Type
    Blog Post
    ...improved fluorescent proteins through mutagenesis. Chapter 6 - The future of FRET and fluorescent protein... addition to creating and sharing many great fluorescent protein tools, Professor Goedhart is very active..., characterizes and uses genetically encoded fluorescent probes. You can follow him on twitter: @joachimgoedhart...Additional Resources from the Addgene Blog Visit our Fluorescent Proteins featured topic page Read Joachim's Series... Series on Choosing the B(right)est Fluorescent Protein Plasmids 101: Visualizing Subcellular Structures...and Organelles Resources at Addgene.org Find Fluorescent Protein Plasmids Find Plasmids for FRET Find ...

  20. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...as the number of citations highly influenced by this paper. Influence is measured by the number of citations...or are highly influenced by the author. You can also see who an author is most influenced by (other authors...learning to extract important features such as influential citations, images, and key phrases. It also takes...help you quickly identify papers with greater influence, the average number of citations per year and ...authors who they cite the most), and who the author influences (who cites the author the most). Best uses Finding...Results in your feed are ranked based on their influence on the network, or body of literature, as a whole...
Showing: 121 - 140 of 472 results