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We narrowed to 342 results for: GAI

Showing: 121 - 140 of 342 results

  1. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...image. You could probably also do Western blotting against GFP expression, but I didn’t. 2. In the original...data not shown). Pro-Tips: 1.Designing primers against genomic DNA to amplify the gRNA targets was challenging...

  2. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ... In this movie Ethan Hawke’s character revolts against class divide that is created by genetics.    Zinc.... The movie sees the USS Enterprise crew fight against a genetically engineered superhuman Khan. The novel...

  5. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...matter whether that other person really is acting against the team’s interests or not; once that seed of ...Guide Management for Scientists: Managing v Leading Gain Management Skills Volunteering at a Professional...

  6. Antibodies 101: Validation

    Type
    Blog Post
    ...These strategies use multiple, unique antibodies against the same protein, each targeting a different epitope...correlation between the results. Using antibodies against different epitopes on the same target reduces the...

  8. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...are not looking for, but there are also benefits gained by lowering the complexity of the screen. For example...Another interesting approach is to design screens against known druggable targets, preferentially those for...

  9. Adenovirus Guide

    Type
    Guide
    ... used viral vector platform for vaccine design against a diversity of viruses. This guide contains many...this, and to the strong host’s immune response against the transduced cells, transgene expression delivered...mammalian hosts and have been used as vaccines against infectious diseases such as COVID-19, Ebola virus... immunity to Ad5 and protect nonhuman primates against ebolavirus challenge . Journal of Virology, 85 ...

  10. Molecular Biology Reference

    Type
    Guide
    ... within bacteria, while allowing for selection against any bacteria not carrying the plasmid. Additionally...out-populate bacteria with plasmids, thus selecting against the propagation of these plasmids through cell ...These attached DNA templates are then amplified again producing ~1,000 copies of each template. Each template...color that is emitted each time a base is added. Again, each base (A,C,T, or G) is labelled with a different...

  11. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...steps and the joined modules cannot be separated again by the same cloning enzymes. MXS (MluI-XhoI-SalI...properties, they generated monoclonal antibodies against them, fused them to GFP, and compared them to popularly...In order to overcome these limitations, Takeharu Nagai and colleagues at Osaka University have developed...

  12. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...increase the chances of inducing an immune response against the capsids or transgenes. Sensory neurons and ... is not neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat...

  13. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ... to screen the NCC-1 library of approved drugs against the entire kit; a parallel analysis that successfully...sgRNA expression vectors (containing ten guides against each of the 8,158 predicted T. gondii protein-coding...

  14. Chemogenetics Guide

    Type
    Guide
    ...olanzapine has also been shown to be an agonist against hM4Di, and is especially attractive for use in ...eaav5282. PMID: 30872534 (Link opens in a new window) Nagai Y, Miyakawa N, Takuwa H, Hori Y, Oyama K, Ji B, ...

  15. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    ...creative scientists will deliver some fabulous photos again this year! Why #DecktheLab? Help us spread some...

  16. We’re Going Viral This Week!

    Type
    Blog Post
    ... the viral service, and now we’re “going viral” again! #AddgeneGoesViral on Twitter Starting today, our...

  17. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    ...scientists delivered some spectacular photos once again this year! All winners receive an Addgene care package...

  18. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...more. We didn’t want to keep the knowledge we’ve gained along the way to ourselves so we decided to share...
Showing: 121 - 140 of 342 results