We narrowed to 134 results for: Green fluorescence protein

...of indels and thus frameshifts that disrupt the protein-coding capacity of a locus. When using S. pyogenes...code for amino acids near the N’ terminus of the protein, in order to mitigate the ability of the cell to...for amino acids close to the C’ terminus of the protein, to maximize the chances of creating a non-functional...nucleotides, restricting gRNAs to 5 – 65% of the protein coding region will still result in many dozens ... a specific edit, such as the insertion of a fluorescent tag or the introduction of a specific mutation...0561-9 Dempster JM, Pacini C, Pantel S, Behan FM, Green T, Krill-Burger J, Beaver CM, Younger ST, Zhivich...

...absence of a protein, determine if a protein is up or downregulated, examine a protein’s stability or ...Hjelm, 1972, Bjorck 1984). Protein A, Protein G, or the recombinant Protein A/G are conjugated to agarose...support while unbound proteins are washed away.  While both Protein A and Protein G have an affinity for...compatible with either Protein A or G.  While commonly used, Protein A and Protein G are not suitable for...applications, such as protein isolation from serum. In this case, Protein A and Protein G will bind both the...of the target protein or interfere with protein interactions of interest. Tagged proteins are typically...modifications, or study how a target protein interacts with other proteins or nucleic acids. Read on to learn...

...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable...tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal protein-interacting RNA aptamers...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages ..., or inducing degradation of the Cas9 protein. Most Acr proteins are specific to a particular type of ...and antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9...fused to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging can... sequences using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to...

...well as the type of transgene delivered: fluorescent proteins, optogenetic sensors, CRISPR elements, etc...BBB via interactions with a similar protein called LY6C1. This protein is expressed in both BALB/cJ mice...exists to prevent substances in the blood, like proteins and viruses, from crossing into the brain. While... it, cross the BBB through an endothelial cell protein called LY6A (Hordeaux et al., 2019; Huang et al...capsids that rely on interactions with those two proteins to cross the BBB are often not effective in NHPs...2023 AAV.CAP-Mac CNS: neurons (rats, infant green monkey, rhesus macaque); vasculature (adult marmosets...astrocytes (adult rhesus macaque) Rats, marmosets, green monkeys, rhesus macaques N/A Chuapoco et al....

... and Resources CRISPR Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit GreenGate Cloning System (Plant...Optogenetics Pertz Lab cpFRET Kit Rinehart Recombinant Phosphoprotein Kit S. cerevisiae Advanced Gateway Destination...

...Photocleavable protein engineered from a green-to-red photoconvertible fluorescent protein 400 UVR8 domains...release proteins from cellular membranes using light. light-induced protein dissociation using protein A fragments...photoreceptors to create protein systems controlled by light. These "photoswitchable" proteins offer exquisite ...of a protein of interest. A genetically-encoded nuclear Cry2 fusion can thus direct a CIB1-protein fusion...Inhibits protein function by reversibly sequestering targets into large, optically assembled protein architectures...transcription using engineered zinc finger proteins, using proteins GIGANTEA (GI) and the light oxygen voltage...pair to the target protein, and the other to the membrane where the target protein is to be sequestered...

...Biosensor AAV Browse our AAV that express fluorescent biosensor proteins, including calcium sensors, under the... the control of various promoters. Sensor Green Calcium Sensors GCaMP8 axon-GCaMP8 soma-GCaMP8 ribo-GCaMP8...

...expression plasmid involved in coexpression of light (green) and heavy (blue) chains as driven by two CMV promoters... moved to direct functional validation by immunofluorescence-immunocytochemistry (IF-ICC.) This method...exciting for scientists working to visualize multiprotein complexes.  Fig. 2. Multiplex immunolabeling...

... BirA, has been used to identify and study protein-protein interactions in proximity labeling techniques...extensive research history and characterization. Many proteins and processes naturally found in E. coli have ...and a tool-kit from the Hamdoun Lab to create fluorescent fusions in a multitude of organisms including...you are working with Arabidopsis check out the GreenGate Toolkit which allows for easy cloning of constructs...

...region encodes three structural capsid proteins or viral proteins (VP1, VP2 and VP3) that form the icosahedral... vectors have been engineered to express fluorescent proteins, often under strong neuron-specific promoters... split inteins, proteins that carry out a naturally occurring process known as protein trans-splicing.... the viral particle and are only composed of a protein capsid and the viral genetic material inside. The...region encodes four non-structural replication proteins (Rep78, Rep68, Rep52, and Rep40), which are involved...chromosome 19. This integration is mediated by proteins Rep78 and Rep68, which can bind to specific Rep-binding...not carry the genes to express Rep78 and Rep 68 proteins and are therefore unable to integrate in the host...

...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable...tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal protein-interacting RNA aptamers...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages ..., or inducing degradation of the Cas9 protein. Most Acr proteins are specific to a particular type of ...and antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9...fused to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging can... sequences using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to...

...assembling bimolecular fluorescence complementation experiments to analyze protein-protein interactions in plants..., synthetic terminators, protein purification tags and fluorescence proteins for use in E. coli for a ...Dupont 95 plasmids including backbones, fluorescent proteins, protein tags, and signal peptides, for use in...elements and fluorescent reporters to be used in combination with the MoClo-YTK toolkit for protein expression...example, a promoter, a 5' UTR, a signal peptide, a protein-coding gene, and a terminator, as in Figure 1)....PhytoBrick parts. MoBiFC - Modular Bimolecular Fluorescence Complementation Toolkit Plant Expression Benjamin...optimize secretion and cell surface display of proteins of interest. Yeast Secrete and Detect Yeast Expression...

...plasmid type, mutations, and/or tags such as fluorescent protein (FP) tags. Information on genes and associated...Omi-mCherry HTRA2 mCherry LTR Parkinson's Douglas Green 50487 pSLIK-NFLAG-hFUS FUS Flag, His TRE ALS Zissimos...pLV-SigmaR1-mNeonGreen SIGMAR1 mNeonGreen CMV ALS Gia Voeltz 226571 siRNA-resistant SigmaR1-mNeonGreen F191A...SigmaR1-mNeonGreen R119A F191A SIGMAR1 mNeonGreen CMV ALS Gia Voeltz 226575 ss-SigmaR1DTM-mNeonGreen SIGMAR1...Multisystem proteinopathy, ALS Nicolas Fawzi 139121 hnRNPA2_LC_NtoS HNRNPA2B1 T7 Multisystem proteinopathy, ALS...Multisystem proteinopathy, ALS Nicolas Fawzi 139123 hnRNPA2_LC_12E HNRNPA2B1 T7 Multisystem proteinopathy, ALS...Multisystem proteinopathy, ALS Nicolas Fawzi 139125 hnRNPF_PLD_StoA HNRNPA2B1 T7 Multisystem proteinopathy, ALS...

...transmembrane proteins that bind a specific ligand. Ligand binding then activates G-proteins to modulate...bioluminescent modules, such as luciferase-fluorescent protein fusions, to more-sensitive opsins has led...The first chemogenetic receptors were based on G-protein coupled receptors (GPCRs) . The largest class of...DREADDs), like RASSLs, are based on engineered G-protein coupled receptors, but unlike RASSLs, show insensitivity...that can be broadly classified by the signaling protein that the receptors couple to: Gq-DREADDs Gq-DREADDs...Gq-DREADDs signal through the Gαq/11 G-protein and activate neuronal firing through stimulating phospholipase...-DREADDs Gi-DREADDs signal through the Gαi/o G-protein and inhibit neuronal signaling by inhibiting adenylate...