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We narrowed to 396 results for: ICL

Showing: 121 - 140 of 396 results

  1. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...stock, followed by resuspension of the collected particles in a smaller volume. Always remove packaging cell...reagents typically enhance the adsorption of a virus particle to a target cell by reducing the repulsive electrostatic...an accurate count of the number of infectious particles in a given viral stock will give you a sense of...

  2. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ...examples: experiment or analysis. In the rest of this article, we'll explain the thinking behind the tool, its...do this internally, but they are not available publicly. Furthermore, we did not find any tools which ...the How To Guide. Conclusion CrispyCrunch is now publicly-available for high-throughput design and analysis...

  3. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    ... modified in the golgi and then transported in vesicles via the secretory pathway to their subcellular...cycle, proteins may be transported through the vesicles to the lysosome where they are finally degraded... hope you find the resources discussed in this article useful for your next localization experiment and...

  4. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...dynamics of vesicle fusion and release from the plasma membrane by fusing various vesicle cargoes to FP... et al. "Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by ...

  5. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...highlight some of the reagents that have been made publicly available through Addgene.  What makes CRISPR... Individual gRNAs are designed in silico using publicly available gRNA design software and synthesized...derivatives of Cas9. Several CRISPR libraries are publicly available through Addgene, with more being added...

  6. Antibody Plasmid Collection

    Type
    Collection
    ... express plasmid-based antibodies. Description Article PI One-step assembly of antibody heavy- and light-chain...

  7. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...1,2), and cell types used for packaging viral particles (6), can influence vector performance. Open science...citable DOIs through DataCite before being made publicly available through the Data Hub. These minimum ...of factors that contribute to recombinant AAV2 particle aggregation and methods to prevent its occurrence...

  8. DNA Purification Without a Kit

    Type
    Blog Post
    ...genomic, or DNA fragments from agarose).    In this article, we will go through the basics of four methods ...milk-like appearance. To make DNA stick to the glass particles in glass milk, gel slices are first placed in ...stick to the glass. After binding DNA, the glass particles are pelleted by centrifugation and washed before...

  9. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...checked out Gal's blog, you should! He's got great articles and insight on the newest tools and techniques...fluorescent microscopy. Read More: All Plasmids 101 Articles Addgene's Fluorescent Protein Guide Note: A. ...Max Juchheim contributed to the writing of this article. ...

  10. Hot Plasmids - November 2023

    Type
    Blog Post
    ...preps in the repository through our Hot Plasmids articles. Today, it's time for another edition of Hot Plasmids...and targeting components to reprogram delivery vehicles like lentiviral vectors, CreVLPs, and eVLPs for...   Figure 2: Modular features of DIRECTED particles where the fusion and targeting components are ...

  12. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...study showed that out of 238 published scientific articles, only 46% could be reproduced. In a recent Nature...notebook requires time and knowledge. In a recent article, we translated our knowledge of electronic lab ...what exactly your lab needs from an ELN (the full article is available here):   Does your lab need to keep...

  13. March for Science

    Type
    Blog Post
    ...education.  Disclaimer: The views presented in this article are those of the author do not represent a formal...politics. Disclaimer: The views presented in this article are those of the author do not represent a formal...having evidence’ on Trump’s wiretap claim The Chronicle of Higher Education: “March for Science” Organizer...

  14. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...derived bacterium Agrobacterium tumefaciens as a vehicle to deliver your gene of interest into a target ...cells. Agrobacterium tumefaciens can be used as a vehicle to deliver your gene of interest (GOI) into plant...deliver CRISPR-Cas9 components into plants. Nanoparticles (carbon nanotubes, Kwak et al., 2019), DNA nanostructures...

  15. TALEN Guide

    Type
    Collection
    ...days. In an open access Nucleic Acids Research article that came out in the spring of 2011, the groups...

  16. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...preps in the repository through our Hot Plasmids articles. Here's what you'll find in this post: Glutamate... (Addgene #236081), then generated lentiviral particles in HEK293T cells with pMD2.G (Addgene #12259) ...the viral preps here, and all plasmids from the article! Furlanis, E., Dai, M., Leyva Garcia, B., Tran...

  17. Viral Vectors

    Type
    Collection
    ... of these plasmids must be present for a viral particle or virion to be produced. Each of these plasmids...

  18. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ... of recombinase-dependent AAV vectors. In this article, we will discuss some of the causes of off-target...off-target expression just due to more overall viral particles getting into those cells. Therefore, the first...CAG are very strong and may require less viral particles or relatively short expression times, where as...

  19. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...Crivianu-Gaita 2016). Get more details on ScFvs in this article: Antibodies 101: Single Chain Fragment Variables...concentration. To get even more details, check out our article The Basics of Western Blotting.    Figure...experimental samples. Get more details on ELISAs in our article Antibodies 101: ELISA (Enzyme-linked Immunosorbent...
Showing: 121 - 140 of 396 results