We narrowed to 395 results for: ICL

... modified in the golgi and then transported in vesicles via the secretory pathway to their subcellular...cycle, proteins may be transported through the vesicles to the lysosome where they are finally degraded... hope you find the resources discussed in this article useful for your next localization experiment and...

...dynamics of vesicle fusion and release from the plasma membrane by fusing various vesicle cargoes to FP... et al. "Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by ...

...examples: experiment or analysis. In the rest of this article, we'll explain the thinking behind the tool, its...do this internally, but they are not available publicly. Furthermore, we did not find any tools which ...the How To Guide. Conclusion CrispyCrunch is now publicly-available for high-throughput design and analysis...

...highlight some of the reagents that have been made publicly available through Addgene.  What makes CRISPR... Individual gRNAs are designed in silico using publicly available gRNA design software and synthesized...derivatives of Cas9. Several CRISPR libraries are publicly available through Addgene, with more being added...

...1,2), and cell types used for packaging viral particles (6), can influence vector performance. Open science...citable DOIs through DataCite before being made publicly available through the Data Hub. These minimum ...of factors that contribute to recombinant AAV2 particle aggregation and methods to prevent its occurrence...

...genomic, or DNA fragments from agarose).    In this article, we will go through the basics of four methods ...milk-like appearance. To make DNA stick to the glass particles in glass milk, gel slices are first placed in ...stick to the glass. After binding DNA, the glass particles are pelleted by centrifugation and washed before...

...checked out Gal's blog, you should! He's got great articles and insight on the newest tools and techniques...fluorescent microscopy. Read More: All Plasmids 101 Articles Addgene's Fluorescent Protein Guide Note: A. ...Max Juchheim contributed to the writing of this article. ...

...preps in the repository through our Hot Plasmids articles. Today, it's time for another edition of Hot Plasmids...and targeting components to reprogram delivery vehicles like lentiviral vectors, CreVLPs, and eVLPs for...   Figure 2: Modular features of DIRECTED particles where the fusion and targeting components are ...

...AAV viral preps for systemic delivery of viral particles that will target the central nervous system, peripheral...

...study showed that out of 238 published scientific articles, only 46% could be reproduced. In a recent Nature...notebook requires time and knowledge. In a recent article, we translated our knowledge of electronic lab ...what exactly your lab needs from an ELN (the full article is available here):   Does your lab need to keep...

...education.  Disclaimer: The views presented in this article are those of the author do not represent a formal...politics. Disclaimer: The views presented in this article are those of the author do not represent a formal...having evidence’ on Trump’s wiretap claim The Chronicle of Higher Education: “March for Science” Organizer...

...derived bacterium Agrobacterium tumefaciens as a vehicle to deliver your gene of interest into a target ...cells. Agrobacterium tumefaciens can be used as a vehicle to deliver your gene of interest (GOI) into plant...deliver CRISPR-Cas9 components into plants. Nanoparticles (carbon nanotubes, Kwak et al., 2019), DNA nanostructures...

...preps in the repository through our Hot Plasmids articles. Here's what you'll find in this post: Glutamate... (Addgene #236081), then generated lentiviral particles in HEK293T cells with pMD2.G (Addgene #12259) ...the viral preps here, and all plasmids from the article! Furlanis, E., Dai, M., Leyva Garcia, B., Tran...

... of recombinase-dependent AAV vectors. In this article, we will discuss some of the causes of off-target...off-target expression just due to more overall viral particles getting into those cells. Therefore, the first...CAG are very strong and may require less viral particles or relatively short expression times, where as...

...Crivianu-Gaita 2016). Get more details on ScFvs in this article: Antibodies 101: Single Chain Fragment Variables...concentration. To get even more details, check out our article The Basics of Western Blotting.    Figure...experimental samples. Get more details on ELISAs in our article Antibodies 101: ELISA (Enzyme-linked Immunosorbent...

... Gray Facilitate the Itch-Scratching Cycle via Article Tac1-Expressing Neurons in the Periaqueductal Gray...

...repository through our hot plasmids articles. These brief articles highlight the main features and applications...plasmid or set of plasmids. We hope that these articles make it easier for you to find and use the plasmids...tool for the yeast community in their open access article. Mülleder M, et al. F1000Res. PubMed PMID: 27830062...

...membrane-anchored version useful for labeling extracellular vesicles. LumiScarlet : A fusion of LumiLuc (ATP-independent...

... for producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging vector such...VSV-G envelope protein for pseudotyping NanoMEDIC particles Akitsu Hotta pHCMV-RabiesG Contains Rabies...

...process not addressed in this article, check out our Help Center article How do I pay by purchase order...