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We narrowed to 469 results for: LARGE

Showing: 121 - 140 of 469 results

  1. Hot Plasmids - October 2020

    Type
    Blog Post
    ...the target gene’s native locus, but introducing large genetic changes in C. elegans with CRISPR can be...(ssODN). So how can you use CRISPR to introduce large FP genes into C. elegans? By using a split FP approach...

  2. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ... immunoglobin component that can be produced in large “copies.” This homogeneity circumvents the batch-to-batch...reproducibility and cost-effectiveness for broad and large quantity uses. We therefore use both rAb and mAB...as non-human primates and humans. Since we need larger-volume antibodies for thicker tissues and whole...

  3. Lab Automation at Addgene

    Type
    Blog Post
    ...the Biomek FxP is it’s customizability. It has a large deck that can house many different types of devices... DNA purification We're developing ways to prep large quantities of DNA because some countries just can...be used for a mini prep, except it does it on a larger scale. Using the BIOROBOT Universal Platform is...

  5. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...In complex metazoans, rapid cell division and large scale cell mobility are essential processes during...body. In contrast, these dynamic processes are largely absent in adult organisms, where tissues structures...adapted to studying other systems in which there is large scale cellular movement throughout an organism. ...

  6. Common Injection Routes in Mice

    Type
    Blog Post
    ...injection site is straightforward, as it targets large muscle groups (usually the thigh), though it is ...theoretically be performed anywhere on the body, somewhere large, flat, and easily accessible (like the back) is ...

  8. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...(~150 kDa) and Y-shaped because it contains two large heavy chains and two small light chains that stick...optimally binding sybody, you want to start out with a large and diverse pool of sybodies that hypothetically...because it has the advantage of starting out with a larger initial starting library size (up to 1012 members...

  9. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...population does not move even after applying very large compensation values (100+), consider what two markers...diagonal line in the top right corner of the plot and large compensation values will not move it (Figure 3)....

  10. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...self-inactivating, which is achieved by deleting a large portion of the 3'LTR in the transfer plasmid. The...through insertional mutagenesis. These risks are largely based upon the vector system used and the transgene...

  12. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...pathways and represent the largest family of human drug targets. To enable large-scale profiling of many ...by endogenous GPCR. Example applications include large-scale pharmacological profiling or interrogation...

  13. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...lentiviral-based CRISPR libraries available at Addgene. The large packaging capacity and stable expression of lentiviral... vivo CRISPR screens. AAV vectors have played a large role in these advancements, due to their many benefits...of molecular pathways. These screens have gotten larger, easier, and more comprehensive thanks to the consistent...

  15. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...the University of Goettingen, and several other large research institutions. The following graphic depicts...institutional and lab servers specifically designed to hold large amounts of data. Although it may be useful to selectively...

  16. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...plasmids in the library.  Spinfection If you have a large number of cells to transduce, you may want to consider...pooled libraries can be intimidating and require a large amount of experimental work, but with this guide...protocol. After functional titer is determined, larger lentiviral preps can be used for screening-scale...

  17. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...novel drugs. Commercial Affairs teams make up a large and diverse group of professionals running the marketing...challenging. For instance, the client might need to get a large amount of market research done to inform a go/no-go...
Showing: 121 - 140 of 469 results