Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 402 results for: Mut

Showing: 121 - 140 of 402 results

  1. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...plasmid of all time, but was found to have a point mutation in the puromycin cassette that makes it less efficient...Cas9, this plasmid expresses Cas9n (a D10A nickase mutant) to nick, rather than cleave DNA. Find pSpCas9n...(BB)-2A-Puro (PX459) V2.0. It contains a point mutation in the puromycin cassette that makes it less efficient...

  3. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...vivo and in vitro, as well as to insert targeted mutations through homologous recombination. To further increase...protoplasts, Xie et. al observed slightly higher mutation rates than they’d previously reported in protoplasts..., they found that 50% of plants had biallelic mutations at all five loci. This highly efficient multiplexing...

  5. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...plasmid are the backbone, inserted gene/functional mutations, antibiotic resistance, restriction sites used...most sequence assembly errors occur), deletions, mutations, in-frame protein fusions, and multiple cloning...cases, we deal with multiple inserts or internal mutations that require more than two sequencing reactions...

  6. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...the majority of the lncRNA locus, scientists can mutate the transcription start site, splice sites, exons...function (Hazan and Bester 2021; Zibitt et al. 2021). Mutating splice sites is particularly effective as it can... Transcription start site (TSS) deletion. (B) Mutations of splice sites. (C) Removal of an exon or a large...

  7. Hot Plasmids February 2024

    Type
    Blog Post
    ... generated a series of StayGold mutants and identified a triple mutant, Y187F, R144I and T155Q, which ...Balasubramanian and colleagues showed the single mutation E138D is sufficient to disrupt dimerization while...

  8. A Look at Addgene's QC Process

    Type
    Blog Post
    ... function of the plasmid. This includes point mutations, frame shifts, missing elements, and nucleotide...plasmid that are less likely to affect function when mutated; elements that are difficult to sequence; approaches...Sequence discrepancies are not always a result of mutations in the plasmid, but can arise due to errors in...

  9. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...tissues with Cas9 and a gRNA designed to target a mutated gene. In a Scientific Reports paper introducing...-transformed cells yielded rates of targeted mutagenesis similar to those achieved by isogenic AdVs encoding...CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells. Sci Rep 4:...

  10. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...knowledge of protein structure and saturation mutagenesis. mK-GO matures from green to red, and the FT ...absorption spectra). Subach et al’s directed mutagenesis also produced the three separate timers shown...3. Verkhusha, Vladislav V., et al. "An enhanced mutant of red fluorescent protein DsRed for double labeling...

  11. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ... during differentiation after they introduced mutations that drive leukemia (Figure 3). Figure...useful for studying the impacts of other cancer mutations and non-genetic diversity in human cancer stem...responses to the acquisition of leukemic driver mutations. Cell Stem Cell, 32(4), 564–580.e6. https://doi.org...

  12. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...this process accounts for a percentage of the mutations seen with TALENs and CRISPR. MMEJ is active during...and Ling Lu. (2016). “Highly efficient CRISPR mutagenesis by microhomology-mediated end joining in Aspergillus...Other Genome Engineering Tools like Sleeping Beauty Mutase Find the Ideal CRISPR Software for Your Experiment...

  14. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...transfer’. Resistance can also arise from random mutations in the genetic sequence that happen to be beneficial... if some didn’t develop, either through random mutation or horizontal gene transfer, means to defend themselves...effective drug targets (ones that are less likely to mutate or change in order for the bacterium to survive...

  15. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...TBD domains, part of the HB domain, and several mutations to improve solubility and stability (Figure 2)...in natural DNA repair pathways and spontaneous mutations.  Here, the researchers were able to target multiple...abscessus either simultaneously or stepwise, achieving mutation efficiencies of up to about 9% of colonies (102...

  16. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...one of the FLP-FRT systems in your experiments. Mutated FRT and loxP sites in Flex Vectors – inversion ...common means to achieve ligand control is to fuse a mutant estrogen receptor (ER) ligand binding domain (LDB...Properties of FLP Recombinase Evolved by Cycling Mutagenesis.” Nature biotechnology 16(7): 657–62. PubMed ...

  17. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...fluorescent tag or the introduction of a specific mutation, one generally relies on homology directed repair...experimental conditions used, led to no detectable mutations (Veres et al., 2014). When working with single-cell...Musunuru K (2014) Low Incidence of Off-Target Mutations in Individual CRISPR-Cas9 and TALEN Targeted Human...

  18. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...Electrochemiluminescent assay for detection of extremely rare mutations based on ligase reaction and bead enrichment."...direct identification of allelic composition of mutated DNA in a mixed population of excess wild‐type DNA...probe for the detection of low-abundance point mutations." Analytical chemistry 86.1 (2013): 900-906. PubMed...

  19. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...vasculature to feed themselves, use the blood stream to commute to and invade multiple organs, and most importantly..., tumor penetration, inadequate concentration, mutation of cell surface targets by tumor cells, are a ...the most important: How will cancers evolve and mutate based on the immunotherapeutics we use on them?...
Showing: 121 - 140 of 402 results