We narrowed to 228 results for: PID

1. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

   Type

   Blog Post

   ...and faster ABEs (Fig. 2). These systems allow for rapid continuous protein evolution through many rounds...

2. Developing a cold tolerant E. coli using specialized chaperones

   Type

   Blog Post

   ...tires give a better grip in the cold, but wear out rapidly in warm weather. Psychrophilic enzymes face very...

3. Antibodies 101: Monoclonal Antibodies

   Type

   Blog Post

   ...against many types of molecules including proteins, lipids, and carbohydrates, in this blog post we’ll focus...

4. Clear and Accessible Writing

   Type

   Blog Post

   ...their field, the answers were primarily negative. Stupid, overwhelmed, discouraged, tired…words that indicated...

5. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

   Type

   Blog Post

   ... in E. coli, SLIC is a cheap, standardized, and rapid multi-part DNA assembly method. Read on to learn...

6. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   ...References Swift S, Lorens J, Achacoso P, Nolan GP. Rapid production of retroviruses for efficient gene delivery...

7. Sharing is Caring: But How to Distribute Open Hardware?

   Type

   Blog Post

   ...of commercially available tools and components. Rapid prototyping techniques such as 3D printing, on-demand...

8. How Addgene Creates Content

   Type

   Blog Post

   ...retiring. CRISPR/Cas9, for example, has gone through rapid change in the past few years. Thus, we are constantly...

9. Why and how to start studying chromatin (with Addgene's help)

   Type

   Blog Post

   ...Gerlich DW Cell. 2017 Aug 24;170(5):956-972.e23 Rapid and phosphoinositol-dependent binding of the SWI...

10. Plasmids 101: Inducible Promoters

    Type

    Blog Post

    ...inducer, like Tet systems, may be activated very rapidly. In contrast, promoter systems that require transcription...

11. Top Requested Lentivirus and AAV of 2016

    Type

    Blog Post

    ...Cusher DS, Maratos-Flier E, Roth BL, Lowell BB. Rapid, reversible activation of AgRP neurons drives feeding...

12. Plasmids 101: Yeast Vectors

    Type

    Blog Post

    ...prokaryotes. This includes but is not limited to: rapid growth, ease of replica plating and mutant isolation...

13. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type

    Blog Post

    ... desired 15 base pair sequence in the genome in rapid fashion. The two remaining plasmids in the kit have...

14. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type

    Protocol

    ...430281 0.45 µm PES complete filtration unit, 500 mL, rapid-flow, VWR 73520-984 Syringes, 30 mL, VWR BD302832...

15. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type

    Blog Post

    ...indicator toolbox, Ed Boyden’s lab developed a way to rapidly screen thousands of proteins for one that can report...

16. Summer SciComm: Preprints

    Type

    Blog Post

    ...asapbio.org/preprint-info ASAPbio, Preprints and Rapid Communication of COVID-19 research: https://asapbio.org...

17. Addgene: The First Twenty Years

    Type

    Blog Post

    ...when CRISPR arrived in 2012, our repository was rapidly able to support sharing of CRISPR constructs and...

18. Viral Vectors 101: An Introduction to AAV

    Type

    Blog Post

    ...in the nucleus and is a good option if you need rapid transgene expression, since you don’t have to wait...

19. To Each HIS Own

    Type

    Blog Post

    ...Lou J, Hipskind RA, Nordheim A, Stunnenberg HG. Rapid and efficient purification of native histidine-tagged...

20. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type

    Blog Post

    ...Forostyan, T., Colosimo, L. K., Graham, T. E. (2015). Rapid parallel measurements of macroautophagy and mitophagy...