We narrowed to 229 results for: PID

1. Science Career Options

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   Blog Post

   ...including: Science Reporter Scientific Sales Epidemiologist Scientific Recruitment Meteorologist Research...

2. Fluorescent Proteins 101: Luciferase

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   Blog Post

   ...reaction compared to using a high-intensity laser to rapidly excite GFP. As a result of the different mechanisms...

3. Individual REAL TALE Repeat Plasmids

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   Collection

   ...Lab plasmids The Joung Lab recently described a rapid and robust protocol for constructing TALE repeat...

4. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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   Blog Post

   ... in E. coli, SLIC is a cheap, standardized, and rapid multi-part DNA assembly method. Read on to learn...

5. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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   ...References Swift S, Lorens J, Achacoso P, Nolan GP. Rapid production of retroviruses for efficient gene delivery...

6. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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   Blog Post

   ...and faster ABEs (Fig. 2). These systems allow for rapid continuous protein evolution through many rounds...

7. Antibodies 101: Monoclonal Antibodies

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   Blog Post

   ...against many types of molecules including proteins, lipids, and carbohydrates, in this blog post we’ll focus...

8. Sharing is Caring: But How to Distribute Open Hardware?

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   Blog Post

   ...of commercially available tools and components. Rapid prototyping techniques such as 3D printing, on-demand...

9. How Addgene Creates Content

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   Blog Post

   ...retiring. CRISPR/Cas9, for example, has gone through rapid change in the past few years. Thus, we are constantly...

10. Developing a cold tolerant E. coli using specialized chaperones

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    Blog Post

    ...tires give a better grip in the cold, but wear out rapidly in warm weather. Psychrophilic enzymes face very...

11. Clear and Accessible Writing

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    Blog Post

    ...their field, the answers were primarily negative. Stupid, overwhelmed, discouraged, tired…words that indicated...

12. Why and how to start studying chromatin (with Addgene's help)

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    ...Gerlich DW Cell. 2017 Aug 24;170(5):956-972.e23 Rapid and phosphoinositol-dependent binding of the SWI...

13. Plasmids 101: Inducible Promoters

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    ...inducer, like Tet systems, may be activated very rapidly. In contrast, promoter systems that require transcription...

14. Top Requested Lentivirus and AAV of 2016

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    Blog Post

    ...Cusher DS, Maratos-Flier E, Roth BL, Lowell BB. Rapid, reversible activation of AgRP neurons drives feeding...

15. Plasmids 101: Yeast Vectors

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    Blog Post

    ...prokaryotes. This includes but is not limited to: rapid growth, ease of replica plating and mutant isolation...

16. Kiran Musunuru on the Newest TALEN Genome-Editing System

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    Blog Post

    ... desired 15 base pair sequence in the genome in rapid fashion. The two remaining plasmids in the kit have...

17. Addgene: The First Twenty Years

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    Blog Post

    ...when CRISPR arrived in 2012, our repository was rapidly able to support sharing of CRISPR constructs and...

18. Viral Vectors 101: An Introduction to AAV

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    Blog Post

    ...in the nucleus and is a good option if you need rapid transgene expression, since you don’t have to wait...

19. To Each HIS Own

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    Blog Post

    ...Lou J, Hipskind RA, Nordheim A, Stunnenberg HG. Rapid and efficient purification of native histidine-tagged...

20. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    Blog Post

    ...indicator toolbox, Ed Boyden’s lab developed a way to rapidly screen thousands of proteins for one that can report...