We narrowed to 630 results for: PROC

...ELISA basics All ELISAs follow the same overall process. First users coat a multiwell plate with a capture...cross-react with samples, eliminating them from the procedure removes one potential source of cross-reactivity... labs can conjugate antibodies themselves, the process can be laborious and expensive. In some cases, ...the bench.   Indirect ELISAs The indirect ELISA process is similar to that of a direct ELISA, but the indirect...antibodies that work well together. Moreover, the procedure is longer than alternative ELISA methods, especially...peptide. The positive control will confirm that the procedure is working and should always be included.  For...

...control point of DNA replication in bacteria, so the process is carefully regulated by a mix of positive and...replication (oriC), where initiation begins. This process relies on the initiation protein DnaA. First, DnaA... versions is required to start the replication process. When enough copies of ATP-DnaA are bound, including...integration host factor (IHF) assists with the process: it bends the DNA to redistribute the DnaA from...plasmid ori, after which replication of the plasmid proceeds (and terminates) similarly to the bacterial chromosome...complexes between iterons on different plasmids in a process called "handcuffing," which blocks replication....

...actually share many biological properties and processes with our own cells. Like human cells, yeast DNA...proteins and tissues to better assess developmental processes. For example, the Stainier lab studied the proliferation...making it ideal to study basic molecular biology processes. C. elegans are also quite amenable to genetic...investigate an array of biological systems and processes including the nervous system, development, cell...β-cells identified by HOTcre mediated labeling." Proceedings of the National Academy of Sciences 106.35 (2009... in freely behaving Caenorhabditis elegans." Proceedings of the National Academy of Sciences 113.8 (2016...

...team as a DNA Sequencing Intern. The application process When I first saw the comprehensive job description...leaving the interview hopeful to continue on in the process and I was excited when I received an email to invite...what my position entailed. The official training process was lined out to span the first three months of...the plasmid function. The steps of the training process gave me enough time to become familiar with the...there. At this point, I have seen several lab processes and am currently working on projects for the Science...

... enter the culture medium during subculturing processes or from the air if the cell line remains exposed... infecting mycoplasma. The primers used in the process are either species/gene-specific or universal. .... Some amount of expertise is required in this process because result interpretation might be difficult...by training eligible staff for the cell culture process and hence eliminate one of the causes of contamination...filtration. There is no 100% assurance that this process can avoid contamination but it’s a very good practice...cultures. Elimination is a very time-consuming process and increases the risk of secondary contamination...

...   While the VGS process isn’t new to Addgene, we are excited to share the procedures and Python scripts... vector (AAV) samples and created a simplified process, termed viral genome sequencing (VGS), to confirm...We recently published a description of the VGS process and use cases in Human Gene Therapy. Existing methods...substrates for tagmentation-based library preparation procedures. This finding eliminates the double-stranding...

...    Figure 1: In the isotype conversion process the variable regions of a parental antibody (blue...isotype (purple, mouse). Depending on the cloning process, all constant regions, just the heavy chain constant...isotype to that of a different group (Fig. 1). This process, called isotope conversion, gives users the flexibility...addition to increasing multiplexing capabilities this process is frequently used to reduce immunogenicity and...therapeutic potential of antibody medicines. In this process, antibodies derived from non-human species like...

...into a plasmid of interest. You can use similar processes to add overhangs to your insert of interest for... The steps following primer design and the PCR process itself are very similar to those outlined in our... with a few quirks specific to the PCR cloning process - please check out that post if you need a more... suited for analyzing the gene’s function. The process is shown graphically in the following figure, in...restriction digestion. As such, the later steps in this process are the same as those discussed in our restriction...

...Allikalt et al., 2020). Impact of labeling process: The process of dye labeling, such as conjugation with...beneficial when studying dynamic processes like mitochondrial dynamics. This process, involving mitochondrial ...specimens has offered illumination on biochemical processes that are crucial in the world of biological research...result in cellular toxicity due to the permeation process. Advanced technologies in fluorescent dyes/ligands...

...laboratories from using these methods for routine processes like plasmid validation. At seqWell, Inc., our...sample-specific barcodes. Rather than continuing to process 100s to 1000s of samples individually, plexWell...additional benefit of plexWell™’s sequential tagging procedure, the quantities of library molecules for each ...a wide range of input DNA concentrations. This process is explained more fully in the animated Fig. 1 ...samples are brought in line with each other before proceeding to library prep. Even with this “pre-prep” normalization...

...bacteria’s thinner membrane. Procedure The stain is a pretty simple process. First, flood the heat-fixed...pneumoniae, while the alcohol used in the staining process bleached another, Klebsiella pneumoniae. After ...photos/occbio/7092385559 For more specific procedural instructions, including timing, preparing the... them red or pink. At the end of the staining procedure, gram positive bacteria are stained purple or ...

...Cas13d CRISPR array processing is robust, with CasRx and either an unprocessed or processed gRNA array (22 ...characteristic of the Cas13 superfamily. Yan et al. proceeded to study orthologs from Eubacterium siraeum (EsCas13d...orthologs described in these papers can independently process their own CRISPR arrays into guide RNAs. crRNA ...

... we had to revamp some of our quality control processes. For the many other COVID-19 related plasmids ... for small batches of samples. As soon as that process is complete, QC scientists prepare Sanger reactions...them inline with our slower, more traditional, QC process. While this has worked well for the early sets ...Ginkgo are striking a balance by modifying certain processes in both core operations. As it has been in much...adapting to a new way of doing things. These new processes have been instrumental in achieving our mission...

... can be used for a diverse array of processes. The food processing industry, for instance, employs psychrophilic...time for a given process. These are extremely useful benefits for any food processing industry as it can...psychrophilic enzymes for milk processing, fruit juice clarification, meat tendering, and oligosaccharide...

...plasmids we distribute through our quality control processes. Every plasmid we receive is rigorously verified...every plasmid we distribute through our deposit process, and then we carefully track samples through our... to find all of the information needed for the process. Always sequence verify Once the physical sample...sequencing If you’re like us, you need a strategy for processing several sequencing reactions in parallel. We ...We have a few tricks to simplify the process. First, nearly all of our Sanger sequencing is done from glycerol...

...deliverables of your work, such as improving a process or procedure, solving a problem, or making a key decision... industry position is the on-site interviewing process. For both of my academic jobs, I interviewed with...different lab groups without having a formal interview process. Industry interviews, however, were more structured...lasted from half to all of the day.  This whole process may seem foreign and a little overwhelming, but...

...plasmid (packaging plasmid). Viral production then proceeds by transfection of all three plasmids into a producer...the host genome as part of the viral infection process (though it can integrate at chromosome 19q13.4 ... via needlestick or cut. Following all sharps procedures for your organization will significantly reduce...best to be well-rested and alert the day of the procedure. Mice move much more quickly than cell cultures...understandably induce some nerves. Following safety procedures and practicing safety culture, however, can let...

...host’s polymerase, but requires Rep proteins to process a double-stranded intermediate into the single-...media are harvested and purified. In total, this process takes 4-7 days, not counting the time needed to...iodixanol and concentrate the purified virus. This process can be completed in one long day, or the virus ... that was carried over from the AAV production process. Either SYBR green technology or a TaqMan primer...generated from an ITR-containing plasmid. This whole process takes ~3 hours to complete: 1 hours hands-on time...

...takes a little bit longer in insect cells. Processing Processing of your baculovirus is straightforward —...cell preparations, which we talk about below. Processing The viral supernatant is typically collected ... couple days to increase yield. The collection process is a cycle of collecting the media and adding fresh... fresh media every 12 to 24 hours. Processing the virus-containing supernatant will depend on the virus...

...often, it is also a bottleneck in the experimental process. The majority of current cloning techniques involve...bacterial recombination pathway to allow any cloning procedure to be performed using a simple two-step, 2 hr ...be immediately adopted by any laboratory. All procedures are performed using the same three step protocol... greater homology, so performing more complex procedures can be aided by extending homologous sequences...routinely screen just two colonies for simple cloning procedures. Use high fidelity DNA polymerase This will minimize...