We narrowed to 559 results for: SEC

...kb. ITRs are inherently unstable due to their secondary structure, palindromic nature, and high GC content...typically fails to sequence through ITRs due to their secondary structure and high GC content, but some companies...sites, and then excise the sequence between the second pair. The final result is a permanently flipped...

...considerable number of researchers, I had high hopes of securing tenure in a British or Irish University where ... courses taken during my PhD and Undergraduate sections and brought in more focus on the list of conferences...towards me as a product and not me as a scientist. Second, I updated my LinkedIn profile, matched it with...

.... This app also has a great educational section. This section holds the Gibco Cell culture manual and ...favorite lab app, please share it in the comments section below! Many thanks to our guest blogger Sean Stacey...

...Xin has been able to develop photoswitchable Intersectin, Cdc42, Raf1, Mek1, Mek2, Cdk5, and Cas9. Xin...consuming in the first case and nonspecific in the second. Fatima therefore set out to design and produce...the amount of HMO originally in the sample. The second sensor is a fusion protein consisting of sfGFP ...

...protein secretion, making harvesting recombinant proteins a cinch. This is because it doesn’t secrete its ...proteins, making the recombinant protein its major secretion. Beyond sheer protein production, K. phaffii is...

...something smokin'? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) here. Here's...option for targeting GFP. But why might you need a second GFP antibody?  Similarities:  Generated against...mEGFP-HRas (from Plasmid 18662). See the Applications section on the Anti-GFP [N86/8R] page for more details....

...overexpression and knockdown. Addgene also has a number of insect vectors to consider. An easy way to see what is...available is to browse Addgene’s Collection under the insect expression system or by filtering by fly as species...organs of interest, such as the intestines, can be dissected at various times throughout the lifespan of the...

... plasmid page under the "Resource Information" section. Analyze your plasmid sequence For more immediate...also click one location in the plasmid and then a second location to select the sequence between the two...what you think about the updates in the comments section below and keep checking in at blog.addgene.org ...

...bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular Cytology and Van Leeuwenhoek Centre...Joachim Goedhart is an assistant professor at the Section of Molecular Cytology and Van Leeuwenhoek Centre...joachimgoedhart.   Marieke Mastop is a PhD candidate at the Section of Molecular Cytology (University of Amsterdam)...

...   Once you get your exciting new antibody, the second thing you’ll want to do is store it away safely...kit, and is readily available on any number of secondary antibodies. And look - if it ain’t broke, don’...fluorophore strength with protein abundance.    Second, you’ll need to consider spectral overlap. Fluorophores...

... Along with charming nighttime displays, these insects have contributed an important tool to the scientific...Peak emission (nm) Size (kDa) Substrate Cofactors Secreted North American firefly (Photinus pyralis) Natural...cell membrane and deliver the substrate; however, secreted luciferase or alternative substrates can permit...

...business competitions. When the team was unable to secure enough money to sustain a startup, he went to work... the data must be reproducible by someone else. Second, "an entrepreneur must be OK with the constant ...or product? Please let us know in the comments section! And read on for more great information about starting...

...bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular Cytology and Van Leeuwenhoek Centre...Joachim Goedhart is an assistant professor at the Section of Molecular Cytology and Van Leeuwenhoek Centre...joachimgoedhart.   Marieke Mastop is a PhD candidate at the Section of Molecular Cytology (University of Amsterdam)...

...PHP.eB compared to the existing ChR2(H134R).  In the second experiment, ChRger2 was delivered and activated...-invasive methods to the neurons in the right secondary motor cortex (M2). Since M2 is located on the ...lab could thin the mice’s skull near the M2 and secure a fiber optic cannula to the skull, leaving the...

..., my mind went completely blank. After several seconds of truly panicked silence, I opened my mouth to... rousing success–the response was a full sixty seconds of silence–but it was thorough and coherent.  To...nerve to do what I did. Until that panicked, split-second decision, I had always thought I had an obligation...

...present. One common example is using pre-adsorbed secondary antibodies (which recognize other antibodies) ...this can increase background staining (see next section), it may be advantageous if you are trying to detect...useful for certain research applications. Many secondary antibodies are polyclonal, as their function is...

...and a line about what I hope to discover. This section may make the difference in whether you get funding... the successful use of your preliminary data to secure further funding. Make it clear that you intend ...your head up! It’s very likely that at first (and second) you won’t succeed, but you must try, try again...

...transcript.   Figure 1: A predicted conserved secondary structure and sequence conserved Rho-independent...contains enzymes that cut RNA not protected by secondary structure. It is thought that poly(A)s are used...example, relies on a specific sequence and RNA secondary structure to induce transcript cleavage, similar...

...bookshelf. Pro tip The “cited by” and references sections of a PubMed entry are great places to look for... that cite the background, methods, or results section is also displayed. Saving papers Papers can be...that contain these terms. You can also create intersects in your feed which means only papers than contain...

...which does not have primary and only contains secondary antibodies, allowing us to only image autofluorescence...independent screening”, where they look at the same section and determine the antibody’s reliability through...neuroanatomy without the fear of referencing a wrong section. The antibody screening and development of a focused...