...systems in the Sashital Lab, Iowa State University. She is actively interested in science communication and...in Two-Step-SHERLOCK or in a fast, high-throughput manner in one step as One-Pot-SHERLOCK. Two-step-SHERLOCK...sequences, Cas12a activity gets activated. This unleashes indiscriminate ssDNA cleavage activity which cleaves...2020).
Detecting coronavirus using CRISPR-based SHERLOCK (Specific High sensitivity Enzymatic Reporter...sensitivity in the zeptomolar range while One-step-SHERLOCK has a sensitivity in the femto-aptomolar range...light illuminator.
Approach: Highly similar to the SHERLOCK diagnostic test, this test varies in the sequence...JG, Gootenberg JS, Abudayyeh OO, Zhang F (2019) SHERLOCK: nucleic acid detection with CRISPR nucleases....