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  1. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ...addition to helping with daily laboratory operations, she also works on improving laboratory efficiency through...samples can be stored in? Different shelves? Different racks on each shelf? Give each container a specific...samples are submitted as bacterial colonies in petri dishes, but close to 80% of samples are received as DNA...large collections. Many companies, such as Thermo Fisher and Micronic, provide 2D barcoded tubes. 1D barcodes...done easily with a barcode reader and an Excel spreadsheet. Start by scanning the sample barcodes into the...and use this ID to name the corresponding Excel sheet.3. Be specific  - When assigning a physical location...
  2. Plasmids 101: Protein tags

    Type
    Blog Post
    ...Annette Sievers, Ph.D., for her tips and guidance. She is way better at protein purification than he is....
  3. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...that control who a female wants to mate with and if she decides to raise their offspring or leave them to... encouraged to not just wait for papers to be published, but to engage in ongoing, real-time dissemination...A paper on stickleback fish science was first published in 1867. It said they were hard to raise because...
  4. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...external video recorder and a mic clipped to her shirt. She rolled several long takes, and we cut it up in post...hopefully they’ll be just as interesting as the finished product for you, the viewer. While you’re here...
  5. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ... Excellence in Molecular Plant Sciences in 2019. She joined Sichuan Normal University and became a lecturer...Originally published Apr 13, 2021 and last updated Aug 12, 2021. This post was contributed by guest bloggers...University, Jieze Zhang, an intern in the Yang Lab, and Sheng Yang, a professor in synthetic biology from the ... optimizing strains with low efficiency.       Sheng Yang obtained his Ph.D. from Shanghai Institute ...biomedical applications.   Yiwen Zhang entered the Sheng Yang Lab of the CAS Center for Excellence in Molecular...
  6. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...with a PhD in reproductive biology and genetics. She loves to communicate the latest and greatest in scientific...companies supply gRNA sequences, such as GenScript, ThermoFisher Scientific and OriGene, or you can order them...
  7. March for Science

    Type
    Blog Post
    ...Systems Biology department at Harvard University. She is a scientist with a passion for photosynthetic ...
  8. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...systems in the Sashital Lab, Iowa State University. She is actively interested in science communication and...in Two-Step-SHERLOCK or in a fast, high-throughput manner in one step as One-Pot-SHERLOCK. Two-step-SHERLOCK...sequences, Cas12a activity gets activated. This unleashes indiscriminate ssDNA cleavage activity which cleaves...2020). Detecting coronavirus using CRISPR-based SHERLOCK (Specific ​H​igh ​s​ensitivity ​E​nzymatic ​R​eporter...sensitivity in the zeptomolar range while One-step-SHERLOCK has a sensitivity in the femto-aptomolar range...light illuminator. Approach: Highly similar to the SHERLOCK diagnostic test, this test varies in the sequence...JG, Gootenberg JS, Abudayyeh OO, Zhang F (2019) SHERLOCK: nucleic acid detection with CRISPR nucleases....
  9. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ... writer at the Institute for Protein Innovation. She writes about all things IPI, from the scientists ...all bind outside the ligand-binding pocket, their sheer size impeding ligand attachment. The five other ...recommended for use in flow cytometry. Based on the established applications of the original hybridoma monoclonals...
  10. Brain Initiative Collection

    Type
    Collection
    ... enhancer element Gordon Fishell 83894-AAV2 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent... enhancer element Gordon Fishell 83894-AAV5 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent... enhancer element Gordon Fishell 83894-AAV9 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent... enhancer element Gordon Fishell 83894-AAVrg pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent...hDlx enhancer element Gordon Fishell 83895-AAV1 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP...hDlx enhancer element Gordon Fishell 83895-AAV2 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP...hDlx enhancer element Gordon Fishell 83895-AAV8 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP...
Showing: 121 - 140 of 594 results