We narrowed to 181 results for: SPEN

1. Making CRISPR Plasmids Using Fragmid

   Type

   Blog Post

   ...but risking the potential of them not working, or spend a few weeks designing and cloning the vectors you...

2. Science communication: 9 Strategies to Get Your Foot in the Door

   Type

   Blog Post

   ...from the British Science Association allow you to spend two to six weeks working at media outlets such as...

3. Gaining Leadership Skills Volunteering at a Professional Organization

   Type

   Blog Post

   ...Committee Chair and Vice Chairs were invited to spend a weekend together, funded by supporters such as...

4. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

   Type

   Blog Post

   ...usually antagonistic and impolite, Complainers, who spend most of their time moaning about their troubles,...

5. Tips for a 1st time AAV user (by a Rookie AAV user)

   Type

   Blog Post

   ...solutions are carefully layered and then the viral suspension generated during AAV production is overlaid. ...

6. 10 Basic tips for mammalian cell culture

   Type

   Blog Post

   ...achieve a consistent growth rate. For every minute spent organizing, an hour is earned (Benjamin Franklin...

7. Transferable Skills: Negotiation

   Type

   Blog Post

   ...student, I was negotiating with my PI on how to spend my grant money (Can I attend this conference?) and...

8. Transferable Skills Guide: Managing a Team

   Type

   Blog Post

   ... did not lack motivation, but he didn’t want to spend hours reviewing papers to piece together the best...

9. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

   Type

   Blog Post

   ... we started, too much time for this project was spent selecting guides, designing primers, and running...

10. Academic vs. Industry Postdocs

    Type

    Blog Post

    ...explore scientifically since you can’t afford to spend too much time working on something extremely risky...

11. Common Injection Routes in Mice

    Type

    Blog Post

    ...injections are most often used when injecting a cell suspension to create xenograft models. Restraint: back scruffing...

12. PITChing MMEJ as an Alternative Route for Gene Editing

    Type

    Blog Post

    ..., but with longer homology arms comes more time spent cloning. In contrast, the short homology required...

13. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type

    Blog Post

    ...and how they work to overcome them, and why she spends some of her time engaging with others on Twitter...

14. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type

    Blog Post

    ...study inflammation and neurodegeneration. He now spends his time learning and writing about science whenever...

15. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type

    Blog Post

    ...game changing nature. Full disclosure: Zimmer also spends some time on Addgene’s role in the rapid dissemination...

16. Negative Can Be Positive: Open AAV Data with Addgene

    Type

    Blog Post

    ...effort to test and optimize our vector - overall, I spent two years testing and reformulating our AAV construct...

17. Transferable Skills Guide: Identifying Your Transferable Skills

    Type

    Blog Post

    ...have already held. Imagine this situation: You’ve spent hours scouring a company’s LinkedIn pages, given...

18. DNA Purification Without a Kit

    Type

    Blog Post

    ...Finally, DNA is precipitated with alcohol and resuspended in TE buffer. After this, the DNA is ready for...

19. A Guide to Starting Your Own Journal Club

    Type

    Blog Post

    ...publications in a specific field(s). Most anyone who has spent any time in academia has likely had the opportunity...

20. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type

    Blog Post

    ...small molecule dyes seems daunting—no one wants to spend their days microinjecting fluorescent conjugates...