We narrowed to 172 results for: TOX;

1. CRISPR 101: Mammalian Expression Systems and Delivery Methods

   Type

   Blog Post

   ...Infects dividing and non-dividing cells. AAV is least toxic method for in vivo viral delivery. Cas9 mRNA and...

2. Light Sheet Fluorescence Microscopy

   Type

   Blog Post

   ...order to improve axial resolution and reduce phototoxicity for imaging live specimens, they combined light...

3. AAVs CREATed for Gene Delivery to the CNS and PNS

   Type

   Blog Post

   ...long-term expression; and 3) they have limited toxicity. However, a key challenge of using AAVs for neuroscience...

4. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

   Type

   Blog Post

   ...the Brunello knock-out library while mitigating toxicity induced by high copy number dsDNA cutting. A Dolcetto...

5. CRISPR Antimicrobials

   Type

   Blog Post

   ...pair change in DNA gyrase, CRISPR phagemids were cytotoxic only for resistant bacteria. Bikard et al. used...

6. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

   Type

   Blog Post

   ...fluorescent dyes of the time was the fact that it was non-toxic and could be expressed in living cells, enabling...

7. Rett Syndrome: A History of Research and Therapeutic Outlooks

   Type

   Blog Post

   ...the Survival of MeCP2-Null Mice without Apparent Toxicity after Intracisternal Delivery. Molecular Therapy...

8. Overcoming the AAV Size Limitation for CRISPR Delivery

   Type

   Blog Post

   ...did so without a substantial immune response or toxicity. More AAV-based CRISPR systems Smaller Cas proteins...

9. Fluorescent Proteins 101: Aptamer Fluorophores

   Type

   Blog Post

   ...visualization technique. Ultimately, MG proved to be too toxic for many in vivo applications, but this work proved...

10. Plasmids 101: Gateway Cloning

    Type

    Blog Post

    ...containing the ccdB gene. Since the ccdB product is toxic to the cell, Gateway cloning efficiency can reach...

11. Plasmids 101: Shuttle Vectors

    Type

    Blog Post

    ...to spontaneously develop resistance to selection toxins. Auxotrophic selection is useful for producing ...

12. Plasmids 101: Stringent Regulation of Replication

    Type

    Blog Post

    ... protein, which can be useful if the product is toxic in high amounts. These plasmids are also useful ...

13. 5 Reasons to Use Reddit for Science Communication

    Type

    Blog Post

    ...career topics. However, many of the posts were about toxic advisors, fruitless job searches, and even despair...

14. Plasmids 101: The Promoter Region – Let's Go!

    Type

    Blog Post

    ...even kill your cells if your gene of interest is toxic. To combat this, scientists have created synthetic...

15. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type

    Protocol

    ...macromolecules. Importantly, iodixanol is inert and non-toxic to mammalian cells. Therefore, and unlike other ...

16. Virus Protocol - Generating Stable Cell Lines

    Type

    Protocol

    ...reduced growth rates, especially if that transgene is toxic. Eventually, the rapidly growing low-level transgene...

17. Fluorescent Protein Guide: Subcellular Localization

    Type

    Collection

    ...B4GALT1(1-61) mTurquoise2 Dorus Gadella 68073 GalToxBFP Golgi apparatus GalT signal anchor transmembrane...

18. Five Popular Model Organisms

    Type

    Blog Post

    ...Yeast models of PD, like those that overexpress the toxic protein α-synuclein (the most common protein found...

19. The Fluorescent Vegetables in Aptamer Soup

    Type

    Blog Post

    ...imidazolinone (DFHBI). DFHBI is membrane permeable and non toxic to cells. Because it rapidly photobleaches, DFHBI...

20. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type

    Blog Post

    ...non-functional, they could be dominant-negative and/or toxic. A potential solution is to include an in-frame ...