...stranded break constructs. Plasmids 171098 and 175570 allow conversion to a dual guide construct. Plasmids ...

...small, repeat-laden DNA regions into a single construct would be technically challenging. Thanks to efforts... of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian...6051):1843-6. Pubmed PMID: 21960622 . Efficient construction of sequence-specific TAL effectors for modulating...

...al., 2018). The ACE reporter is a bicistronic construct that consists of a mutated mCherry and a downstream...fluorescence. To do this they generated a silent GFP construct, that contains an ACG start codon mutation, and...

...labs how they can benefit from depositing their constructs to the repository, and getting feedback from ...

...assay, and easy to purify using histidine-tagged constructs and routine protein and molecular biology techniques... window) Acknowledgments The MCC and plasmid construction was funded by NSF IUSE 1726932 and NSF RCN-UBE...

...Molecular Cell Find the Nme2Cas9 All-in-One AAV constructs CRISPR interference in Candida albicans A new...

... reducing the time required to generate multi-construct transgenic plants. In the most practical terms...

...two-photon illumination than previous GEVIs. Some constructs are also available as viral vectors (here and...and voltage sensor Ace2N and additional GCaMP6 constructs: pAAV-CAG-dLight1.2 (AAV1) pGP-AAV-syn-FLEX-...

... of different studies. Elly Tanaka’s lab has constructed several vectors for use in the axolotl including...GreenGate Toolkit which allows for easy cloning of constructs specifically designed for use in Arabidopsis....

...Institute, they expect to add another 200 or 250 constructs to the kit in the next two years. References...

...plasmids you'll note that those are lentiviral constructs. In 2007 we continued to see requests grow for...

...documentation allow one to efficiently assemble TALEN constructs with custom repeat arrays. TALEN Kits TALEN Kit...Pawel Pelczar pCAG-T7-TALEN(Sangamo)-Destination constructs were designed for optimal mammalian expression...Gate TALEN 2.0 1000000030 Yamamoto Lab TALEN Construction and Evaluation Accessory Pack Takashi Yamamoto...

... a quick way to generate many mutated plasmid constructs. The second post was contributed by Addgene Scientist...

...for additional constructs TetR On Geley Transactivators (tTA or rtTA) Find a construct that expresses ...Tetracycline-inducible Empty Backbones Find a construct that will allow you to insert and induce your ...

... locus. Doyon Tagging Plasmid: 3xFLAG-2xSTREP Construct for integrating at the AAVS1 "safe harbor" locus...pathway, instead of HDR, which makes it easy to construct a donor vector, because MMEJ utilizes very short...method to tag endogenous proteins by using donor constructs that harbor synthetic short homology arms. Notably...

...mice pre-CRISPR was very arduous. First, you’d construct a plasmid with your desired changes, long homology...template, you'd use ivTRT to create the ssDNA construct as described in Figure 2.  You can also use Addgene...

...The aim of these meetings is to give honest, constructive feedback, set tangible research and career goals...

...complex (Rssc) gene knockout and complementation constructs - Lahaye Lab Vaccinia virus ORF library : Expression...MoClo-YTK : Assembly of single and multi-gene constructs for S. cerevisiae - Dueber Lab Yeast Prototrophy...

...hypothesis that the lower expression levels of SunTag constructs would avoid negative effects on cell physiology...

...and tracking cells that express both the dCas9 construct and the fluorescently-labeled gRNA multiplex. ...