We narrowed to 306 results for: construct

... Addgene’s collection of empty backbones for constructing fluorescent fusions. Transcription Reporter:...at Addgene. Cell marking/selection: Expression constructs like plasmids often include GFP as a marker to...

...documentation allow one to efficiently assemble TALEN constructs with custom repeat arrays. TALEN Kits TALEN Kit...Pawel Pelczar pCAG-T7-TALEN(Sangamo)-Destination constructs were designed for optimal mammalian expression...Gate TALEN 2.0 1000000030 Yamamoto Lab TALEN Construction and Evaluation Accessory Pack Takashi Yamamoto...

...experiments. You may have to design several different constructs to find one that could be used for the purpose...monomeric Cerulean mTurquoise2 Cyan Mammalian Constructs to target mTurquoise2 to various subcellular ...

... locus. Doyon Tagging Plasmid: 3xFLAG-2xSTREP Construct for integrating at the AAVS1 "safe harbor" locus...pathway, instead of HDR, which makes it easy to construct a donor vector, because MMEJ utilizes very short...method to tag endogenous proteins by using donor constructs that harbor synthetic short homology arms. Notably...

...complex (Rssc) gene knockout and complementation constructs - Lahaye Lab Vaccinia virus ORF library : Expression...MoClo-YTK : Assembly of single and multi-gene constructs for S. cerevisiae - Dueber Lab Yeast Prototrophy...

...al., 2018). The ACE reporter is a bicistronic construct that consists of a mutated mCherry and a downstream...fluorescence. To do this they generated a silent GFP construct, that contains an ACG start codon mutation, and...

...genome editing, and two of our top-performing constructs are now available through Addgene (pKb-TnpB1 ...as the core nuclease could help keep the total construct size smaller than Cas9 alone. In our recent study...

...labs how they can benefit from depositing their constructs to the repository, and getting feedback from ...

...Molecular Cell Find the Nme2Cas9 All-in-One AAV constructs CRISPR interference in Candida albicans A new...

... reducing the time required to generate multi-construct transgenic plants. In the most practical terms...

...Finger Arrays for Zebrafish Genes - Browse pre-constructed zinc fingers targeting specific genes Oligomerized...

... of different studies. Elly Tanaka’s lab has constructed several vectors for use in the axolotl including...GreenGate Toolkit which allows for easy cloning of constructs specifically designed for use in Arabidopsis....

...two-photon illumination than previous GEVIs. Some constructs are also available as viral vectors (here and...and voltage sensor Ace2N and additional GCaMP6 constructs: pAAV-CAG-dLight1.2 (AAV1) pGP-AAV-syn-FLEX-...

...cell types, while shRNA and siRNA are synthetic constructs introduced through external methods. shRNA processing...C. (2006). Design and cloning strategies for constructing shRNA expression vectors. BMC Biotechnology,...

...Institute, they expect to add another 200 or 250 constructs to the kit in the next two years. References...

...plasmids you'll note that those are lentiviral constructs. In 2007 we continued to see requests grow for...

...Find fluorescent protein fusion constructs and cloning backbones from the Michael Davidson Lab....

... a quick way to generate many mutated plasmid constructs. The second post was contributed by Addgene Scientist...

...conformational change in the sensing region of the construct leads to a change in signal of a circularly permuted...different binding affinities or negative-control constructs used to characterize the sensor's behavior in...biosensor in a novel context or modifying an existing construct, as fluorescence properties can be affected by...fluorescent protein biosensor for maltose Rapid construction of metabolite biosensors using domain-insertion...fluorescent protein biosensor for trehalose Rapid construction of metabolite biosensors using domain-insertion...for popular biosensors in different expression constructs? Browse the table below or search for a biosensor...Yeast GPCR-sensor Toolkit includes plasmids for constructing highly-tunable GPCR-based biosensors in S. cerevisiae...

...mice pre-CRISPR was very arduous. First, you’d construct a plasmid with your desired changes, long homology...template, you'd use ivTRT to create the ssDNA construct as described in Figure 2.  You can also use Addgene...