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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 310 results for: construct

Showing: 121 - 140 of 310 results

  1. Chemogenetics Plasmids

    Type
    Collection
    ... our inventory of chemogenetic constructs, including DREADD constructs....

  2. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...stranded break constructs. Plasmids 171098 and 175570 allow conversion to a dual guide construct. Plasmids ...

  3. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...experiments. You may have to design several different constructs to find one that could be used for the purpose...monomeric Cerulean mTurquoise2 Cyan Mammalian Constructs to target mTurquoise2 to various subcellular ...

  4. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...assay, and easy to purify using histidine-tagged constructs and routine protein and molecular biology techniques... window) Acknowledgments The MCC and plasmid construction was funded by NSF IUSE 1726932 and NSF RCN-UBE...

  7. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ...labs how they can benefit from depositing their constructs to the repository, and getting feedback from ...

  8. TALEN Plasmids and Kits

    Type
    Collection
    ...documentation allow one to efficiently assemble TALEN constructs with custom repeat arrays. TALEN Kits TALEN Kit...Pawel Pelczar pCAG-T7-TALEN(Sangamo)-Destination constructs were designed for optimal mammalian expression...Gate TALEN 2.0 1000000030 Yamamoto Lab TALEN Construction and Evaluation Accessory Pack Takashi Yamamoto...

  9. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...Molecular Cell Find the Nme2Cas9 All-in-One AAV constructs CRISPR interference in Candida albicans A new...

  11. CRISPR Plasmids - Tagging

    Type
    Collection
    ... locus. Doyon Tagging Plasmid: 3xFLAG-2xSTREP Construct for integrating at the AAVS1 "safe harbor" locus...pathway, instead of HDR, which makes it easy to construct a donor vector, because MMEJ utilizes very short...method to tag endogenous proteins by using donor constructs that harbor synthetic short homology arms. Notably...

  12. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...cell types, while shRNA and siRNA are synthetic constructs introduced through external methods. shRNA processing...C. (2006). Design and cloning strategies for constructing shRNA expression vectors. BMC Biotechnology,...

  13. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...two-photon illumination than previous GEVIs. Some constructs are also available as viral vectors (here and...and voltage sensor Ace2N and additional GCaMP6 constructs: pAAV-CAG-dLight1.2 (AAV1) pGP-AAV-syn-FLEX-...

  14. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ... of different studies. Elly Tanaka’s lab has constructed several vectors for use in the axolotl including...GreenGate Toolkit which allows for easy cloning of constructs specifically designed for use in Arabidopsis....

  17. Microbiology Resources

    Type
    Collection
    ...complex (Rssc) gene knockout and complementation constructs - Lahaye Lab Vaccinia virus ORF library : Expression...MoClo-YTK : Assembly of single and multi-gene constructs for S. cerevisiae - Dueber Lab Yeast Prototrophy...

  18. Editor's Choice, August 2016

    Type
    Blog Post
    ... a quick way to generate many mutated plasmid constructs. The second post was contributed by Addgene Scientist...

  19. COVID-19 Resources

    Type
    Collection
    ...expression Spike protein constructs for making VSV pseudo virus Mammalian constructs Due to the size and complexity...reverse transcriptase, protease, and Bst polymerase constructs now available from Andrea Pauli's lab. Neutralizing...

  20. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...mice pre-CRISPR was very arduous. First, you’d construct a plasmid with your desired changes, long homology...template, you'd use ivTRT to create the ssDNA construct as described in Figure 2.  You can also use Addgene...
Showing: 121 - 140 of 310 results