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We narrowed to 494 results for: control

Showing: 121 - 140 of 494 results

  1. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ...access to transcriptional regulatory elements is controlled during development using the Drosophila wing ...the target protein. This targeted digestion is controlled by the addition of calcium, which MNase requires...only for a short period of time, thus precisely controlling the amount of cutting and mitigating noise generated...

  2. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    .... Layers of control have also been added to the CRISPR technology to allow time-controlled edits to occur...generation of knockout cell lines which has made this control step extremely accessible. For example, let’s say...

  3. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...target sequence lower the repression level. By controlling the number of mismatches we can obtain a range...reporters, mCherry and sfgfp in this case, can be controlled using a plasmid‐borne CRISPR array coding for...

  4. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...-phosphorylated or ‘wild type’ control protein. The ‘wild type’ control can be made one of two ways. First...-containing GFP that can be used as a positive control during optimization experiments. This version of...expression plasmid. This is a particularly important control when creating a phosphoserine-incorporation mutant...mutating a threonine residue to a phosphoserine) to control for effects of the amino acid substitution. Alternatively...

  5. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...on the left, your digested DNA (and undigested controls) are loaded at the top of the gel in wells positioned...to insert. It is critical to set up a negative control ligation reaction with no insert DNA added. This...minimum, two transformations after a ligation: 1. Control Transformation containing the ligation mixture ...see any colonies, you should conduct a positive control to ensure that your transformation worked. You ...depending on the number of background colonies on your control plate (the more background, the more colonies you...

  6. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...qPCR assays, samples, standard curves, and normal controls must be run in triplicate.  The nice thing about... of DNA in your sample relative to your normal control - you can set up your calculations so that your...so forth. This also serves as a light technical control - an RQ value of 1.5 means you have either made... does not require a standard curve or a normal control, it can take a few hours longer than qPCR to run...

  7. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ... Transcription Reporter: Placing GFP under the control of a promoter of interest can be used to effectively...Scientists can use light to detect, measure, and control molecular signals, cells, and groups of cells in...Plasmids of this type may have the GFP under the control of an additional promoter from that of the gene...fate studies. It can also be used, when put under control of promoters of interest, to visualize the developmental...

  8. Deep Dive: qPCR

    Type
    Blog Post
    ...tips on probe chemistries, primer optimization, controls, and analysis.  As a rule of thumb, you can assume...the fold change of each sample compared to your control. But as we said previously, just because it’s used...assumes the amplification kinetics of the target and control genes are fairly equal, an assumption that is not... Pfaffl et al No Standard Curve sample=control Gentle, et. al No Raw Data researcher defines...between 0 and 1. Flipping your experimental and control values during analysis can allow you to see this...differences between your experimental values and your controls. ANOVA, t-test, multiple regression analysis, ...-018-26707-8Suzuki T; Higgins PJ; Crawford DR. Control selection for RNA quantitation. Biotechniques. ...

  9. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...the origin of replication (ori) and all of its control elements. The ori is the place where DNA replication...depending on how they are regulated. Generally, control of replication is referred to as "relaxed" or "...Vectors Copy Number+ ori Incompatibility Group Control pUC ~500-700 pMB1 (derivative) A Relaxed pBR322...

  10. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ...One of the main tenets of our quality control (QC) mantra is to do our utmost to ensure that scientists...Gene Therapy. Existing methods for AAV quality control Historically, AAV vector QC has been sparse including...number of AAV preparations this level of quality control is sufficient but for viral vector core facilities...

  11. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...placing FP timer DsRed-E5 under the control of Neurog3 (a gene controlling pancreatic differentiation) in ...synthesized or long-lived transcription factors control gross changes in gene expression as new body sections...Pratik, et al. "A programmable synthetic lineage-control network that differentiates human IPSCs into glucose-sensitive...

  12. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...target genes. The sequence of the promoter region controls the binding of the RNA polymerase and transcription... all the time while others are more carefully controlled. Regulated promoters might act only in certain... light. In the cell, promoters themselves are controlled by still other regulatory factors: enhancers,...induced. Requires T7 RNA polymerase, which is also controlled by lac operator. Can be induced by IPTG. Commonly...

  13. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region ...promoters can be regulated by positive or negative control. Positive inducible In the OFF state, the promoter...system, the activator rtTA (reverse tetracycline-controlled transactivator) is normally inactive and cannot...

  14. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...streamline identification. While the Zhang lab controlled their multiplicity of infection so that a single...disease depends on both genetic and expression control systems to mimic illness on a molecular level. ...either with or without mCherry and GFP fluorescent controls. Alternatively, you can order individual plasmids...

  15. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    ...plasmids Anti-CRISPR proteins for optogenetic control of CRISPR-Cas9 Anti-CRISPR proteins that inhibit...has adapted anti-CRISPR proteins for optogenetic control of CRISPR-Cas9. By combining AcrIIA4, a Streptococcus...

  16. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...(4; brighter than control, 3; same as control (Cetn1/sgRNA1), 2; darker than control, 1; very dark). Brighter...mammalian cells was being explored and it allowed control over each individual component of the system. However...

  17. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...opposite chamber) [2]. Laser activation is usually controlled automatically using motion capture software such...targeted brain region after stimulation compared to controls without Chr2. c-Fos is a reliable and widely used...PMC4181379. 3. Kheirbek, Mazen A., et al. "Differential control of learning and anxiety along the dorsoventral ...silencer: development and application to optogenetic control of non-human primate cortex." Frontiers in systems...

  19. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...experiment. Research applications Gene expression control Researchers are always wanting to manipulate gene...depending on the virus used. All of these expression control mechanisms require a specific modification of the...that the copies of library cargo delivered are controllable. For many screening applications, it is essential...

  20. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...of using loxP sites is the inability to tightly control which loxP sites recombine if more than two are...allowing for two separate recombination events to be controlled in parallel. Have you used the Cre-lox system...PubMed Central PMCID: PMC49604. 4. Independent control of immunoglobulin switch recombination at individual...
Showing: 121 - 140 of 494 results