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We narrowed to 200 results for: crispr cas9 grna plasmid

Showing: 121 - 140 of 200 results

  1. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing...need Cas9 plasmid Chen and Wente 42250 DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid Joung...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... CRISPR Zebrafish CRISPR Plasmids: Zebrafish...following CRISPR plasmids have been designed for use in zebrafish. Cut Fully functional CRISPR/Cas enzymes...CRISPR plasmids that have been designed for use in zebrafish. CRISPR...

  2. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Validated In Resistance Co-expressed Cas9 Depositing...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... CRISPR Yeast CRISPR Plasmids: Yeast Browse ... following CRISPR plasmids have been designed for use in yeast. Cut Fully functional CRISPR/Cas enzymes...If the plasmid that you choose does not also express a gRNA, you will need to use a separate gRNA expression...If the plasmid you’re using does not also express a gRNA, you will need to use a separate gRNA expression...

  3. Viral Vectors 101: Preparing Pooled Libraries

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    Blog Post
    ...single plasmid transformation, though, a pooled library has hundreds to millions of distinct plasmids, all...anyone who has worked with individual plasmids knows, some plasmids amplify well and others don’t. The size...Resources and references References Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...screening, and validation of CRISPR libraries. The Genome Engineering using CRISPR/Cas Systems Forum- Moderated...Lentiviral CRISPR Libraries- A nice overview of what to consider when using pooled lentiviral CRISPR libraries...Brigham MD, Sanjana NE, Zhang F. Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...Addgene, including cDNA, barcoding, screening, and gRNA libraries. Most of the pooled libraries at Addgene...

  4. CRISPR Plasmids - Mammalian Expression

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...CRISPR Plasmids: Mammalian Expression The following CRISPR plasmids have been designed for use in mammalian...NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Browse CRISPR Plasmids By Function...If the plasmid that you choose does not also express a gRNA, you will need to use a separate gRNA expression...If the plasmid you’re using does not also express a gRNA, you will need to use a separate gRNA expression...expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter ...

  5. CRISPR Plasmids - Tagging

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    Collection
    ...and Myers Tagging Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...Tagging Plasmids: pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...proteins. This CRISPR/Cas plasmid-system consists of two components: A vector containing Cas9 and a validated...validated gRNA, based on the Zhang lab's PX458 . Multiple gRNA plasmids may be used. The HDR (homology directed...donor plasmid with homology arms and selection marker. The first deposited plasmids in this CRISPR-Cas ...

  6. CRISPR Plasmids - Base Edit

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Two...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...CRISPR Plasmids: Base Edit Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit... or search the tables below for CRISPR base editing plasmids. Plasmids are available for expression in...are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase...CRISPR plasmids for precisely editing DNA bases without inducing a DNA break. CRISPR...editors are available with multiple Cas9 variants including high fidelity Cas9’s. Further advancements have ...

  7. CRISPR Plasmids - Prime Edit

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Prime...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...nicking sgRNA), cloning enzyme, selectable marker, and whether the plasmid contains Cas9. ID Plasmid Expression... CRISPR Prime Edit CRISPR Plasmids: Prime Edit...or search the tables below for CRISPR prime editing plasmids. Plasmids are available for expression in...find a gRNA vector based on expression system, promoter, the type of gRNA (e.g., pegRNA, epegRNA, nicking...Edit Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation...

  8. Plasmids 101: Screens vs. Selections

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    Blog Post
    ... Addgene Blog CRISPR libraries Genome-wide screens with CRISPR Lentiviral CRISPR libraries ... With the advent of CRISPR, genome-wide knockout screens are possible with gRNA libraries targeting all....S. Genetic screens in human cells using the CRISPR/Cas9 system. Science, 343(6166): 80-84 (2014). doi...While the entire genome can be knocked out with CRISPR, the entirety of the small molecule realm is typically...downstream manipulations as a forward or reverse screen (CRISPR, drug, etc.), just with an added baseline component...component. Alternative screening techniques While CRISPR and drugs dominate the screening scene, there are... worth considering. RNAi screens are similar to CRISPR, but instead of knocking out the genes of interest...

  9. CRISPR Plasmids - dCas9-FokI

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A...effects of CRISPR. Browse, sort, or search the tables below for CRISPR-FokI plasmids. Plasmids are available...FokI CRISPR: dCas9-FokI Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR...Drosophila. Mammalian ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter...Yeast ID Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids that should...

  10. CRISPR History and Development for Genome Engineering

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    Collection
    ...like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution shows no...out Addgene’s CRISPR Guide and CRISPR blog posts , or find CRISPR plasmids for your research. Addgene has... CRISPR CRISPR History CRISPR History and Development for Genome Engineering You may also like...specificity. Figure 2: An overview of CRISPR and NHEJ/HDR. The Cas9/gRNA complex produces a DSB at the target...sequencing, CRISPR is a robust system for genome-wide screening. CRISPR Technological Advances CRISPR technology...with paired gRNAs to lower off-target cleavage frequency. Truncated gRNAs: Truncated gRNAs display less...High fidelity Cas9s: Three high fidelity Cas9 variants , SpCas9-HF, eSpCas9, and HypaCas9 display very ...

  11. CRISPR Plasmids - gRNAs

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    Collection
    ... CRISPR gRNAs CRISPR Plasmids: gRNAs Browse CRISPR Plasmids...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...using any of these gRNA plasmids and review the publication associated with each plasmid for more information...PAM sequence. Which CRISPR application is this gRNA sequence compatible with? CRISPR knockout experiments...and unpublished plasmids containing gRNA sequences designed to be used with any CRISPR nuclease and function... The table below lists gRNA plasmids designed to target various genes or genomic regions. It is highly...

  12. CRISPR Plasmids - Plants

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...of Cas-containing plasmids. ID Plasmid Promoter Cloning Enzyme Co-expressed Cas9 Cas9 System Selection ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... CRISPR Plants CRISPR Plasmids: ...following CRISPR plasmids have been designed for use in plants. Cut Fully functional CRISPR/Cas enzymes...If the plasmid that you choose does not also express a gRNA, you will need to use a separate gRNA expression...If the plasmid you’re using does not also express a gRNA, you will need to use a separate gRNA expression...

  13. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    Blog Post
    ... to make linearized vector, the Cas9 enzyme along with a specific gRNA was used to cleave the 22kb vector...A, Li K, Wang B, Jin S, Reiser M, Lockey RF. CRISPR/Cas9 nuclease cleavage combined with Gibson assembly...mixing and matching of parts. Gibson assembly meets CRISPR Gibson can be adapted to more complicated cloning...use of Gibson assembly combined with the popular CRISPR technology and is described in a recent publication...second example of using Gibson Assembly along with CRISPR was also described recently (Jiang et al. 2015)...chromosome (up to 100 kb) were specifically cut out via CRISPR and then assembled into a vector using Gibson assembly...homology with the bacterial chromosome fragments. CRISPR cutting was used to circumvent the need to PCR ...

  14. Plant Plasmids and Resources

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    Collection
    ... Plasmid Collections Plant Plasmids Plant Plasmids and Resources...biology - deposit plant plasmids with Addgene! CRISPR Please refer to Addgene's CRISPR Guide for a general...introduction to CRISPR technology or the Plant CRISPR Resources for a full selection of plasmids expressing...functional variants of Cas9 and empty gRNA backbones. A few examples of useful plant CRISPR resources include...assembly of one or more gRNA expression cassettes - Qi-Jun Chen. Cas9 and gRNA backbone for use in rice... The MoClo CRISPR/Cas Toolkit for Plants includes CRISPR/Cas nucleases, base editors, gRNA backbones, ... an intronized Cas9 series of vectors and DNA modules for assembly of CRISPR/Cas9 constructs - Sylvestre...

  15. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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    Blog Post
    ...Read all CRISPR blog posts Resources on Addgene.org Browse our CRISPR interference plasmids Find bacterial...bacterial CRISPR plasmids Read our CRISPR guide ...treated with a folA gRNA were more sensitive to trimethoprim when compared to control gRNA treated or untreated...Mobile-CRISPRi into bacteria. Top: A Tn7 transposon containing the CRISPRi components and a plasmid with...ready to move Mobile-CRISPRi into your bacteria of interest? Find Mobile-CRISPRi plasmids at Addgene! References...about other CRISPR methods for bacterial genome engineering   Learn about human CRISPRko, CRISPRi, and CRISPRa...them. For example, gene knockdown with CRISPR interference (CRISPRi) has been limited to lab-adapted bacteria...

  16. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    Blog Post
    ... by combining specific sgRNAs with Cas9 or Cas9 derivatives. Unmodified Cas9 causes double stranded ...genome-wide screening using CRISPR Resources on Addgene.org Find CRISPR plasmids and resources here Find...generation libraries for genome-wide CRISPRa, CRISPRi, and CRISPR-Cas9 knockout genetic screens." bioRxiv...et al. "Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation." Nature biotechnology..."Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9." Nature biotechnology...vemurafenib resistance in A375 cells. The SAM system (CRISPR/Cas9 Synergistic Activation Mediator) uses an improved... transcriptional activation by an engineered CRISPR-Cas9 complex." Nature517.7536 (2015): 583. PubMed ...

  17. CRISPR Plasmids - Cascade-Cas3

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Cas3...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... CRISPR Cas3 CRISPR Plasmids: Cascade-Cas3... or search the tables below for CRISPR Cascade-Cas3 plasmids. Plasmids are available for expression in...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions. CRISPR...Cascade-Cas3 Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...to express equally. To combat this, some CRISPR-Cas3 plasmids take an all-in-one approach and include ...

  18. Lentiviral Prep Service

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    ...information on CRISPR plasmids and function, see our CRISPR plasmids resource page . Cas9 for Editing Genomic...Root Cas9 Lentivirus Cas9 viruses can be used to interrogate the genome in a variety of ways. Cas9 proteins...guide (gRNA) that harbors targeting sequences. Browse our repository for a list of gRNA plasmids targeting... 52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein...backbone. Zhang Cas9 and Accessories for Activating Gene Expression Catalytically-dead Cas9 (dCas9) can be fused...Lentivirus Browse In-Stock Lentivirus Pooled CRISPR Libraries Cas9 Pooled Barcoding Libraries Control Addgene's...are made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive...

  19. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...sequential homology directed repairs (HDR) with CRISPR/Cas9. Donor A contains: 400bp homology arms to the...increasing the size of the AAV donor template for CRISPR-mediated homology directed repairs (HDR). Split... for integration, “part A” of the transgene, the gRNA target site that mediated the donor template’s integration...integration; and a sequence of stuffer DNA after the gRNA target site. Donor B contains: 400 bp homology ...single step and serve as donors for two consecutive CRISPR-mediated HDR events which results in the integration...if non-homologous end joining (NHEJ) disrupts the gRNA target site after the first HDR event, then the ...A in any of the reading frames downstream of the gRNA target site so that transcripts undergo nonstop ...

  20. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ...PubMed. Find plasmids from this publication at Addgene. Other strategies for CRISPR/Cas9 editing in C...of 2013, a remarkable nine papers describing CRISPR/Cas9 genome engineering methods for C. elegans were...approach Multiple features of C. elegans have made CRISPR/Cas9 so successful in this species, namely, the robust...to create a new and efficient selection-based CRISPR/Cas9 method to generate knock-ins on any genetic background...template plasmid to create fluorescent and 3xFLAG-tag fusion proteins. The repair template and Cas9-sgRNA...other species or in cell culture. In addition to CRISPR/Cas9 applications, SEC selection could replace other...system represents another leap forward for the CRISPR/Cas9 revolution!   References Streamlined Genome ...
Showing: 121 - 140 of 200 results