Technique: Probe Phage Genomes for Host Binding Proteins
Type
Blog Post
...nitrocellulose membranes and exposed to IPTG in order to induce protein expression. Cells on the membranes will ...orientation of each membrane on its plate before moving on. Then peel each membrane off the plate (saving...high salt.
To probe membranes with target bacterial cells, wash your membranes with PBST to remove salt... on the membrane to bind their target cells, pulling them out of solution. Wash your membranes well with... 20 Petri dish-sized circles of nitrocellulose membrane and press each gently down onto a plate of colonies...regrow the lifted colonies overnight), flip the membrane over and place it colony-side up onto a fresh ...plasmid selection. Remove air bubbles between the membrane and the agar using forceps. Several protein induction...