...into therapeutically relevant, non-transformed mammalian cells. These viral vector systems also work equally... well in dividing and quiescent, post-mitotic mammalian cells. Applications of CRISPR delivery by adenoviral... WM, Curiel DT (2019) Long-term correction of hemophilia B using adenoviral delivery of CRISPR/Cas9. Journal...

...meet may all be scientists but not all will be familiar with the specifics of your research. Try to avoid...introduce yourself to a scientist whose work you are familiar with. You can tweak your elevator pitch slightly...website or the program booklet. Make sure you’re familiar with the policies before posting something on ...

... your poster session, or it may be held at an unfamiliar venue. Finding your poster board well ahead of...find him/her on social media or through their affiliations’ website. Time permitting, you can go back to...Exchange business cards or jot down their name and affiliation from the poster heading to get in touch at a ...

...near the beginning or end of a reaction is often unreliable, we suggest using the “Somewhat similar sequences... that you can extract practically every single reliable base pair from your results. This option is not...searches Once you have used blastn to determine the reliable portion of a Sanger sequencing result and noted...

...parts have been carefully engineered to support reliable functional composition. In other words, these ...and to the design community: If you can begin to reliably put together 10-gene systems, what do you want...3 May 2013). Vivek Mutalik et al. "Precise and reliable gene expression via standard transcription and...

...cell wall synthesis as its mode of action (sound familiar?). Carb is degraded by beta-lactamase, just like...other plasmids must carry both a bacterial and a mammalian antibiotic selection gene. However, it does require...Pros: Widely available, cost-effective, confers mammalian resistance to G418. Con: Slow transformation ...

...dominant homology-directed repair (HDR) pathway in mammalian cells, but a less frequent DNA repair mechanism...suggestions. Disable the other repair mechanisms Mammalian cells have 2 primary repair mechanisms besides...advances in CRISPR/Cas9-mediated knock-ins in mammalian cells. J Biotechnol., 16(4): 387-390 (2020). 10.1016...

... and biolistic bombardment of immature embryos (Liang et al. 2017).  PEG-mediated methods, while technically...technique often comes down to the researcher’s familiarity with different systems.  Using Cas9-gRNA RNPs... 29668265. PubMed Central PMCID: PMC6002863. 4. Liang, Zhen, et al. "Efficient DNA-free genome editing...

...Cpf1 orthologs that display cleavage activity in mammalian cells. Like Cas9 nucleases, Cpf1 family members...Cpf1 nucleases would exhibit in vivo activity in mammalian cells. From 16 diverse Cpf1 candidates, Zetsche...efficiency of two Cpf1 orthologs known to be active in mammalian cells, LbCpf1 and AsCpf1. In both reports, on ...

...your training? These could serve two purposes – familiarize you with the techniques you will be learning ...you get started!) Are there protocols you can familiarize yourself with? If your bench mentor hasn’t sent...the protocol we’ll be using next week so I can familiarize myself with it.” It won’t be a bother to them...

...simple, portable, and inexpensive platforms to reliably detect nucleic acids at the atomolar level. The... easy to transport and work rapidly, providing reliable results in as little as an hour. Find the SHERLOCK...The Zhang lab demonstrated that SHERLOCK could reliably distinguish between Zika and a closely-related...

...COVID-19 (Joung, Ladha et al., 2020). Cas13a in mammalian cells The Zhang lab made the exciting discovery...discovery that some Cas13a orthologs can function in mammalian and plant cells (Abudayyeh et al., 2017). They...superfolder GFP fusion to stabilize the protein in mammalian cells. LwaCas13a-msfGFP can mediate cleavage with...

...leave them to their own devices. Chytrid fungi (Lillian Fritz-Laylin) Chytrid fungi are useful for the ...so can be used to study aspects of cognition. Spiralians - Crepidula atrasolea (Jonathan Henry) This black...they have only 1,700 genes total). Hydra (Celina Juliano) This is a known stem cell and regeneration model...

...proper controls are invariably inconclusive and unreliable. Proper controls provide the constant variables...backbone as Plasmid A—that does not target any mammalian gene. This non-targeting control is critical to.... Replicate controls: technical and biological Reliable results are reproducible from properly controlled...

... There are a couple different approaches to be familiar with when talking about IF, direct and indirect...antibody that you consider in order to determine how reliable the antibody seems and what sort of protocol you..., will help make antibodies more effective and reliable in the future.  We hope that this post has shed...

...have been using AAV vectors for gene editing in mammalian cells since 1998. In that year, Russell and Hirata...zygotes and thereby correct a mutation that causes Hemophilia B. Unfortunately, work combining CRISPR with ...postnatal administration of AAV vector cures haemophilia B mice." Scientific Reports 7.1 (2017): 4159....

...expressing proteins in bacteria, yeast, insect and mammalian cells. Check out Kay et al., 2009 for methods ...purification of tagged transcription factors in mammalian cells and transgenic mice.” PNAS 100.13 (2003)..., M., & Ting, A. Y. “Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin...

...bloggers Dominik Paquet and Dylan Kwart from Ludwig-Maximilians-University in Munich and Marc Tessier-Lavigne...to be incorporated into the edited genome. In mammalian cells such as stem cells, HDR is relatively rare...Institute for Stroke and Dementia Research, Ludwig-Maximilians-University Munich. His lab develops iPSC-based...

...care about. Don’t try to present topics that are unfamiliar to you because the company you are visiting works...unless you are certain that the audience will be familiar with the terms. If you feel you need to define...lecture.This takes practice (see above) and a deep familiarity with your content. If at all possible, practice...

...45), there are also initiatives to create more reliable reagents for future research. I’ve talked to many...many researchers who have complaints about the unreliability of antibodies. IPI is working to address this...repeat previously published results. This over-reliance on the perfect story for a “high impact” publication...