We narrowed to 532 results for: lias

... collection to perform a functional screen in mammalian cells to identify any solute channels that can...team will be able to validate their findings in mammalian cells with both human and murine targets thus ...says. In induced pluripotent stem cells derived endothelial cells from women who are missing an entire X ...

...research technician in Kaspar Podgorski’s lab at Janelia Research Campus, HHMI where he works on developing...collaborative and highly interactive environment that Janelia offers,” says Aggarwal.In addition to the biosensors...pooled library of mutant sensors expressed in a mammalian cell line engineered to be electrically excitable...

... and have a diverse range of functions within mammalian cells. Here, we will cover how these structures...putting DNA at risk. Consequences of duplexed RNA Mammalian cells have multiple receptors in place to sense...strands to re-pair. The two dominating RNases in mammalian cells (RNase H1 and H2) handle most of these intermediates...

...supplements), suggested sequencing primers, or mammalian selection markers. You don’t need a fancy database...feature you want to verify because you won’t get reliable sequence data back for the first 50-100 base pairs... Julian Taylor-Parker...

...pGFP-Rab8A Primary cilia Rab8 EGFP Maxence Nachury 35623 pEGFPN3-Sstr3 Primary cilia Sstr3 EGFP Kirk Mykytyn...pEGFPN3-5ht6 Primary cilia Htr6 EGFP Kirk Mykytyn 104358 pEGFP-N-Drd1 Primary cilia (Neurons) Drd1 EGFP... ensconsin GFP William Bement 26742 EMTB-mCherry Microtubules Ensconsin mCherry William Bement 164460 ...Mitochondrial targeting sequence (COX8) mStayGold Janelia Integrative Imaging 189774 p4xmts-mScarlet3_N1 ...79990 pKeratin-miRFP703 Intermediate filaments (epithelial) Keratin miRFP703 Vladislav Verkhusha 79991 pmiRFP703...-mGold2t-Keratin-N-17 Intermediate filaments (epithelial) Keratin mGold2t Francois St-Pierre 231767 pCMV-mGold2s-Keratin-N...-mGold2s-Keratin-N-17 Intermediate filaments (epithelial) Keratin mGold2s Francois St-Pierre 26737 GFP-UtrCH...

...neuron-specific expression (hSyn promoter) and pan-mammalian cell expression (CAG promoter). An improved version...neuron-specific expression in both zebrafish and mammalian systems. The GCaMP6, jGCaMP7, and jGCaMP8 sensors...neuron-specific, astrocyte-specific (GCaMP6 only) or mammalian cell expression, and compatible with constitutive...

...system can be used in many cell types including endothelial cell-pericyte, neuron-astrocyte, and cancer-stromal...induce DSBs within minutes. New CRISPR plasmids for Borrelia burgdorferi from Christine Jacobs-Wagner’s lab...enzymes. CRISPR-ARE is a new system from Jiazhang Lian’s lab that enables simultaneous gene activation, ...

...into therapeutically relevant, non-transformed mammalian cells. These viral vector systems also work equally... well in dividing and quiescent, post-mitotic mammalian cells. Applications of CRISPR delivery by adenoviral... WM, Curiel DT (2019) Long-term correction of hemophilia B using adenoviral delivery of CRISPR/Cas9. Journal...

... your poster session, or it may be held at an unfamiliar venue. Finding your poster board well ahead of...find him/her on social media or through their affiliations’ website. Time permitting, you can go back to...Exchange business cards or jot down their name and affiliation from the poster heading to get in touch at a ...

...meet may all be scientists but not all will be familiar with the specifics of your research. Try to avoid...introduce yourself to a scientist whose work you are familiar with. You can tweak your elevator pitch slightly...website or the program booklet. Make sure you’re familiar with the policies before posting something on ...

...novel techniques or to engineer improved tools for familiar applications. But how does one invent a tool for...Fusing EGFP to the self-labeling FlavinTag made a reliable one-component magnetoresponsive system that could...clearly visible whether expressed in E. coli, in mammalian cells, or in purified form, and didn’t need any...

...near the beginning or end of a reaction is often unreliable, we suggest using the “Somewhat similar sequences... that you can extract practically every single reliable base pair from your results. This option is not...searches Once you have used blastn to determine the reliable portion of a Sanger sequencing result and noted...

...parts have been carefully engineered to support reliable functional composition. In other words, these ...and to the design community: If you can begin to reliably put together 10-gene systems, what do you want...3 May 2013). Vivek Mutalik et al. "Precise and reliable gene expression via standard transcription and...

... and biolistic bombardment of immature embryos (Liang et al. 2017).  PEG-mediated methods, while technically...technique often comes down to the researcher’s familiarity with different systems.  Using Cas9-gRNA RNPs... 29668265. PubMed Central PMCID: PMC6002863. 4. Liang, Zhen, et al. "Efficient DNA-free genome editing...

...cell wall synthesis as its mode of action (sound familiar?). Carb is degraded by beta-lactamase, just like...other plasmids must carry both a bacterial and a mammalian antibiotic selection gene. However, it does require...Pros: Widely available, cost-effective, confers mammalian resistance to G418. Con: Slow transformation ...

...dominant homology-directed repair (HDR) pathway in mammalian cells, but a less frequent DNA repair mechanism...suggestions. Disable the other repair mechanisms Mammalian cells have 2 primary repair mechanisms besides...advances in CRISPR/Cas9-mediated knock-ins in mammalian cells. J Biotechnol., 16(4): 387-390 (2020). 10.1016...

...Cpf1 orthologs that display cleavage activity in mammalian cells. Like Cas9 nucleases, Cpf1 family members...Cpf1 nucleases would exhibit in vivo activity in mammalian cells. From 16 diverse Cpf1 candidates, Zetsche...efficiency of two Cpf1 orthologs known to be active in mammalian cells, LbCpf1 and AsCpf1. In both reports, on ...

...COVID-19 (Joung, Ladha et al., 2020). Cas13a in mammalian cells The Zhang lab made the exciting discovery...discovery that some Cas13a orthologs can function in mammalian and plant cells (Abudayyeh et al., 2017). They...superfolder GFP fusion to stabilize the protein in mammalian cells. LwaCas13a-msfGFP can mediate cleavage with...

...simple, portable, and inexpensive platforms to reliably detect nucleic acids at the atomolar level. The... easy to transport and work rapidly, providing reliable results in as little as an hour. Find the SHERLOCK...The Zhang lab demonstrated that SHERLOCK could reliably distinguish between Zika and a closely-related...

...leave them to their own devices. Chytrid fungi (Lillian Fritz-Laylin) Chytrid fungi are useful for the ...so can be used to study aspects of cognition. Spiralians - Crepidula atrasolea (Jonathan Henry) This black...they have only 1,700 genes total). Hydra (Celina Juliano) This is a known stem cell and regeneration model...