...fluorescence microscopy, flow cytometry, or spectrophotometry. The range of fluorophores available for antibody... et al., 2007; Stoeckius, et al., 2017). Metal isotopes are useful for mass cytometry (Bendall, et al....Modifications in Cells and Tissues in Situ. Curr Protoc Cell Biol 89:e115. https://doi.org/10.1002/cpcb...Detecting antigens by quantitative immuno-PCR. Nat Protoc 2:1918–1930. https://doi.org/10.1038/nprot.2007.267...

...-radiative transfer of energy (i.e. no emitted photons) from the excited donor fluorophore to the acceptor...Donor fluorophore excitation by absorption of a photon 2. Energy transfer from the excited donor directly... acceptor fluorophore--think of it as a virtual photon 3. Relaxation of the acceptor fluorophore back ...back to its ground state by emission of a photon with wavelength specific to the acceptor In order for FRET...

...instance, we use a mutant known as CX51 that exhibits motor defects above 25 degrees. Computer scientists and...CRISPR/cas9 to build the mutants, analyse their motor behaviour, and ultimately submit our new strains...our final target decisions, we intend to focus on motor neuron knockouts so that we can perturb factors ...our members took an Arduino microcontroller and a motor controller and made it spin again!  We’ve also learned...

...thinking of an amazing project. You’ve written your protocols, designed your experiments, and prepared your ...purifications, or ligations. The general Gateway cloning protocol is fairly flexible and adaptable to cloning different... they will come with comprehensive and tested protocols, as well as premixed and ready-to-use reagents...101 eBook Resources on Addgene.org0 Check out protocols for plasmid cloning Find vector backbones Read...

...cell level as they are in vitro. Factors like photobleaching, lack of red-shifted indicators, and brightness... linked to a chromophore and functions as a photoreceptor in the eye. There are two flavors of rhodopsin...issue with these GEVIs? They perform poorly in two-photon conditions, which are necessary conditions for ...Addgene.org Addgene’s Viral Services Addgene’s Virus Protocols Addgene’s Chemogenetics Guide Resources on the...

...details on this procedure check out our PCR protocol page, protocol video and reference on how to design PCR...Resources on Addgene.org Protocol: Polymerase Chain Reaction (PCR) Protocol: How to Design a primer  ...

...order to study the conformational dynamics of the motor protein kinesin-1, the Twelvetrees Lab took advantage... molecules, which are much brighter and more photostable than fluorescent proteins, making them ideal ...versions. ASAP5 can be imaged using one- or two-photon microscopy to detect action potentials and subthreshold...fluctuations in awake behaving mice through two-photon microscopy at depths up to 600 µm. Figure...

... targeting vectors. Addgene depositor Takashi Yamamoto’s lab has harnessed MMEJ to create a new method...previous work, Sakuma et al. describe a detailed protocol for MMEJ-mediated knock-in of a GFP-Puro cassette...much shorter sequence homologies. Abbreviated protocol for PITCh Step 1: Generate microhomologies in ...TALENs and CRISPR-Cas9 with the PITCh systems.” Nat Protoc. 11(1): 118-33. PMID: 26678082. Find the plasmids...

...guidelines here. A detailed transformation protocol, as well as protocols for growing Arabidopsis are also available...more than one construct at a time. For detailed protocols, volumes, spin speeds, recipes, and Agrobacterium...your insertion in the absence of selection. For a protocol for antibiotic selection, check out Harrison et...

... tries to “optimize” a particular standard lab protocol. For example, someone may attempt super quick ...quick incubation times in a Western Blot protocol and then blame the antibody when no signals are obtained...so many things which can go wrong in a lengthy protocol. And customers may tell all sorts of details about...reviewing customer data sets, troubleshooting protocols, analyzing information, and solving customer problems...

...enhanced propensity for movement and activity called locomotor sensitization. This enhanced movement is thought...craving (‘seeking drugs’).  What’s involved in locomotor sensitization? Previous research has shown that... previously suggested inhibitory mechanism of locomotor sensitization and might open new ways for a novel...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

...crisis. I like how they are using protocols.io to create a protocol community! Asclepias syriaca and Asclepias...solicitation requires funded labs to rapidly disseminate protocols, methods, data, and tools that result from their...Chisholm) This cyanobacterium is the smallest photosynthetic cell in existence, but most abundant cell on...

...that the protocol is not approved for clinical use at this point, they hope that the protocol serves as...et al., 2018). Now, the Zhang lab has shared a protocol for using SHERLOCK to detect SARS-CoV-2 RNA. The... Singh J, Miao X, Streithorst JA, Granados A, Sotomayor-Gonzalez A, Zorn K, Gopez A, Hsu E, Gu W, Miller...

...GFP can exist in a protonated and a deprotonated state (Tsien, 1998). The deprotonated state has an absorbance... and it emits light that peaks at 508 nm. The protonated state, however, does not absorb light at 488 ...

...FSC/SSC), the detected photons can be represented through a graph of photocurrent vs. time. The resulting...electric potential that can be applied to the photomultiplier inside the cytometer to increase the electric...-mouse CD8b-PerCP-Cy5.5), which is present on cytotoxic T killer cells but not dendritic cells. Using ...

...intensity, caused partially by fluorophore photoinstability, as well as the tendency of fluorophores to...ideal characteristics (low aggregation, high photostability, and high stability after fixation.) From the...cells in which the construct is expressed. The photostability of Brainbow-3.2 is improved due to the addition...of neural circuits, such as connections between motor neurons and the neuromuscular junction. In zebrafish...

...fabulous photos again this year! Why #DecktheLab? Help us spread some cheer by snapping a photo of you... Festivus— you name it, we want to see it! All photos tagged with #DeckTheLab throughout the month of...

...significantly cheaper. When using a homemade plasmid prep protocol, it cost less than $10 to make 1000 lanes worth...ladder? You can find the Tan Lab’s DNA ladder prep protocol here and the pPSU1 and the pPSU2 plasmids are ...Use the Kit-Free Alkaline Lysis Plasmid Miniprep Protocol Learn How to Analyze Your Restriction Digests ...

... new ways to learn about science careers, lab protocols, and, of course, plasmids. Today we’re trying ...Domain Constructs Kit Hot Plasmid 4: The Yeast Prototrophy Kit from Markus Ralser's Lab -  Use this kit ...strains.  Listen to Our Segment on The Yeast Prototrophy Kit Special thanks to our sound engineer/producer...

...Halloween festivities for years – searching our photo archives I found pictures back through 2009, though...contests were indeed occurring earlier but that the photo evidence was destroyed to protect the innocent revelers...Facebook and checkout our FB page on Friday for more photo evidence.        ...